Estudio comparativo de la detección de SARS CoV-2 por RT-PCR en muestras de hisopado nasofaringeo y saliva: un estudio piloto en Bolivia

Myrna Virreira; Shirley Herbas; Licyel Paulas; Magaly Espinoza; Jean Jacques Letesson; Nicolas Dauby; Patricia Rodriguez

doi:10.47993/gmb.v45i2.538

Estudio comparativo de la detección de SARS CoV-2 por RT-PCR en muestras de hisopado nasofaringeo y saliva: un estudio piloto en Bolivia

Translated title of the contribution: Comparative study of SARS CoV-2 detection by RT-PCR in nasopharyngeal swab and saliva samples: a pilot study in Bolivia

Myrna Virreira, Shirley Herbas, Licyel Paulas, Magaly Espinoza, Jean Jacques Letesson, Nicolas Dauby, Patricia Rodriguez

Research Unit in Biology of Microorganisms

Research output: Contribution to journal › Article › peer-review

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Abstract

Objectives: Nasopharyngeal swab sampling for the detection of SARS-CoV-2 is a standard method for the diagnosis of COVID-19, but its collection usually causes discomfort in the patient and exposes healthcare workers to a higher risk. Saliva seems to be a good alternative to nasopharyngeal swabs, as it is non-invasive, reduces the risk of contamination of healthcare workers, and allows self-collection. This study aims to compare the ability to detect SARS-CoV-2 by RT-PCR in the same patient using saliva and nasopharyngeal swab samples to analyze the concordance of the results obtained between the two samples. Methods: Thirty saliva and nasopharyngeal swab samples from patients with COVID-19 symptoms who were admitted to the emergency department of the Viedma Clinical Hospital were taken in parallel. Both samples were analyzed by RT-PCR for the detection of SARS-CoV-2. The concordance of results was calculated using the Cohen's Kappa coefficient. Results: Our results show that there is good concordance (Kappa index 0.730; 95% CI: 0.486-0.974) between the two types of samples analyzed. Conclusions: Saliva seems to be a reliable and effective sample for the detection of SARS-CoV-2.

Translated title of the contribution	Comparative study of SARS CoV-2 detection by RT-PCR in nasopharyngeal swab and saliva samples: a pilot study in Bolivia
Original language	Spanish
Pages (from-to)	99-103
Number of pages	5
Journal	Gaceta Medica Boliviana
Volume	45
Issue number	2
DOIs	https://doi.org/10.47993/gmb.v45i2.538
Publication status	Published - 2022

Keywords

COVID 19
nasopharyngeal swab
RT-PCR
saliva
SARS CoV2

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title = "Estudio comparativo de la detecci{\'o}n de SARS CoV-2 por RT-PCR en muestras de hisopado nasofaringeo y saliva: un estudio piloto en Bolivia",

abstract = "Objectives: Nasopharyngeal swab sampling for the detection of SARS-CoV-2 is a standard method for the diagnosis of COVID-19, but its collection usually causes discomfort in the patient and exposes healthcare workers to a higher risk. Saliva seems to be a good alternative to nasopharyngeal swabs, as it is non-invasive, reduces the risk of contamination of healthcare workers, and allows self-collection. This study aims to compare the ability to detect SARS-CoV-2 by RT-PCR in the same patient using saliva and nasopharyngeal swab samples to analyze the concordance of the results obtained between the two samples. Methods: Thirty saliva and nasopharyngeal swab samples from patients with COVID-19 symptoms who were admitted to the emergency department of the Viedma Clinical Hospital were taken in parallel. Both samples were analyzed by RT-PCR for the detection of SARS-CoV-2. The concordance of results was calculated using the Cohen's Kappa coefficient. Results: Our results show that there is good concordance (Kappa index 0.730; 95% CI: 0.486-0.974) between the two types of samples analyzed. Conclusions: Saliva seems to be a reliable and effective sample for the detection of SARS-CoV-2.",

keywords = "COVID 19, nasopharyngeal swab, RT-PCR, saliva, SARS CoV2",

author = "Myrna Virreira and Shirley Herbas and Licyel Paulas and Magaly Espinoza and Letesson, {Jean Jacques} and Nicolas Dauby and Patricia Rodriguez",

year = "2022",

doi = "10.47993/gmb.v45i2.538",

language = "Espagnol",

volume = "45",

pages = "99--103",

journal = "Gaceta Medica Boliviana",

issn = "1012-2966",

publisher = "Universidad Mayor de San Simon",

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T2 - un estudio piloto en Bolivia

AU - Virreira, Myrna

AU - Herbas, Shirley

AU - Paulas, Licyel

AU - Espinoza, Magaly

AU - Letesson, Jean Jacques

AU - Dauby, Nicolas

AU - Rodriguez, Patricia

PY - 2022

Y1 - 2022

N2 - Objectives: Nasopharyngeal swab sampling for the detection of SARS-CoV-2 is a standard method for the diagnosis of COVID-19, but its collection usually causes discomfort in the patient and exposes healthcare workers to a higher risk. Saliva seems to be a good alternative to nasopharyngeal swabs, as it is non-invasive, reduces the risk of contamination of healthcare workers, and allows self-collection. This study aims to compare the ability to detect SARS-CoV-2 by RT-PCR in the same patient using saliva and nasopharyngeal swab samples to analyze the concordance of the results obtained between the two samples. Methods: Thirty saliva and nasopharyngeal swab samples from patients with COVID-19 symptoms who were admitted to the emergency department of the Viedma Clinical Hospital were taken in parallel. Both samples were analyzed by RT-PCR for the detection of SARS-CoV-2. The concordance of results was calculated using the Cohen's Kappa coefficient. Results: Our results show that there is good concordance (Kappa index 0.730; 95% CI: 0.486-0.974) between the two types of samples analyzed. Conclusions: Saliva seems to be a reliable and effective sample for the detection of SARS-CoV-2.

AB - Objectives: Nasopharyngeal swab sampling for the detection of SARS-CoV-2 is a standard method for the diagnosis of COVID-19, but its collection usually causes discomfort in the patient and exposes healthcare workers to a higher risk. Saliva seems to be a good alternative to nasopharyngeal swabs, as it is non-invasive, reduces the risk of contamination of healthcare workers, and allows self-collection. This study aims to compare the ability to detect SARS-CoV-2 by RT-PCR in the same patient using saliva and nasopharyngeal swab samples to analyze the concordance of the results obtained between the two samples. Methods: Thirty saliva and nasopharyngeal swab samples from patients with COVID-19 symptoms who were admitted to the emergency department of the Viedma Clinical Hospital were taken in parallel. Both samples were analyzed by RT-PCR for the detection of SARS-CoV-2. The concordance of results was calculated using the Cohen's Kappa coefficient. Results: Our results show that there is good concordance (Kappa index 0.730; 95% CI: 0.486-0.974) between the two types of samples analyzed. Conclusions: Saliva seems to be a reliable and effective sample for the detection of SARS-CoV-2.

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Estudio comparativo de la detección de SARS CoV-2 por RT-PCR en muestras de hisopado nasofaringeo y saliva: un estudio piloto en Bolivia

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