ERK activation upon hypoxia: involvement in HIF-1 activation

Emmanuel Minet, Thierry Arnould, Gaetan Michel, Isabelle Roland, Denis Mottet, Martine Raes, José Remacle, Carine Michiels

Research output: Contribution to journalArticlepeer-review


Hypoxia-inducible factor-1 (HIF-1) is a transcription factor activated by hypoxia. The HIF-1 activation transduction pathway is poorly understood. In this report, we investigated the activation of extracellular regulated kinases (ERK) in hypoxia and their involvement in HIF-1 activation. We demonstrated that in human microvascular endothelial cells-1 (HMEC-1), ERK kinases are activated during hypoxia. Using dominant negative mutants, we showed that ERK1 is needed for hypoxia-induced HIF-1 transactivation activity. Moreover, using a kinase assay and Western blot experiments, we showed that HIF-1α is phosphorylated in hypoxia by an ERK-dependent pathway. These results evidence the role of mitogen-activated protein kinase in the transcriptional response to hypoxia. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalFEBS Letters
Issue number1
Publication statusPublished - 18 Feb 2000


  • Extracellular regulated kinase 1 and 2 (ERK)
  • Hypoxia
  • Hypoxia-inducible factor-1 (HIF-1)


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