Electronic and molecular properties of an adsorbed protein monolayer probed by two-color sum-frequency generation spectroscopy

Laurent Dreesen, Christophe Humbert, Yannick Sartenaer, Yves Caudano, Cedric Volcke, Alaa Addin Mani, André Peremans, Paul Thiry, Sophie Hanique, Jean-Marie Frère

Research output: Contribution to journalArticle

Abstract

Two-color sum-frequency generation spectroscopy (2C-SFG) is used to probe the molecular and electronic properties of an adsorbed layer of the green fluorescent protein mutant 2 (GFPmut2) on a platinum (111) substrate. First, the spectroscopic measurements, performed under different polarization combinations, and atomic force microscopy (AFM) show that the GFPmut2 proteins form a fairly ordered monolayer on the platinum surface. Next, the nonlinear spectroscopic data provide evidence of particular coupling phenomena between the GFPmut2 vibrational and electronic properties. This is revealed by the occurrence of two doubly resonant sum-frequency generation processes for molecules having both their Raman and infrared transition moments in a direction perpendicular to the sample plane. Finally, our 2C-SFG analysis reveals two electronic transitions corresponding to the absorption and fluorescence energy levels which are related to two different GFPmut2 conformations: the B (anionic) and I forms, respectively. Their observation and wavelength positions attest the keeping of the GFPmut2 electronic properties upon adsorption on the metallic surface.
Original languageEnglish
Pages (from-to)7201-7207
Number of pages7
JournalLangmuir
Volume20
Issue number17
Publication statusPublished - 2004

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molecular properties
Green Fluorescent Proteins
Monolayers
Spectroscopy
Color
proteins
Proteins
color
Electronic properties
electronics
spectroscopy
Platinum
platinum
Molecular Probes
Electron transitions
molecular electronics
Electron energy levels
Conformations
Natural frequencies
Atomic force microscopy

Cite this

Dreesen, L., Humbert, C., Sartenaer, Y., Caudano, Y., Volcke, C., Mani, A. A., ... Frère, J-M. (2004). Electronic and molecular properties of an adsorbed protein monolayer probed by two-color sum-frequency generation spectroscopy. Langmuir, 20(17), 7201-7207.
Dreesen, Laurent ; Humbert, Christophe ; Sartenaer, Yannick ; Caudano, Yves ; Volcke, Cedric ; Mani, Alaa Addin ; Peremans, André ; Thiry, Paul ; Hanique, Sophie ; Frère, Jean-Marie. / Electronic and molecular properties of an adsorbed protein monolayer probed by two-color sum-frequency generation spectroscopy. In: Langmuir. 2004 ; Vol. 20, No. 17. pp. 7201-7207.
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abstract = "Two-color sum-frequency generation spectroscopy (2C-SFG) is used to probe the molecular and electronic properties of an adsorbed layer of the green fluorescent protein mutant 2 (GFPmut2) on a platinum (111) substrate. First, the spectroscopic measurements, performed under different polarization combinations, and atomic force microscopy (AFM) show that the GFPmut2 proteins form a fairly ordered monolayer on the platinum surface. Next, the nonlinear spectroscopic data provide evidence of particular coupling phenomena between the GFPmut2 vibrational and electronic properties. This is revealed by the occurrence of two doubly resonant sum-frequency generation processes for molecules having both their Raman and infrared transition moments in a direction perpendicular to the sample plane. Finally, our 2C-SFG analysis reveals two electronic transitions corresponding to the absorption and fluorescence energy levels which are related to two different GFPmut2 conformations: the B (anionic) and I forms, respectively. Their observation and wavelength positions attest the keeping of the GFPmut2 electronic properties upon adsorption on the metallic surface.",
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Dreesen, L, Humbert, C, Sartenaer, Y, Caudano, Y, Volcke, C, Mani, AA, Peremans, A, Thiry, P, Hanique, S & Frère, J-M 2004, 'Electronic and molecular properties of an adsorbed protein monolayer probed by two-color sum-frequency generation spectroscopy', Langmuir, vol. 20, no. 17, pp. 7201-7207.

Electronic and molecular properties of an adsorbed protein monolayer probed by two-color sum-frequency generation spectroscopy. / Dreesen, Laurent; Humbert, Christophe; Sartenaer, Yannick; Caudano, Yves; Volcke, Cedric; Mani, Alaa Addin; Peremans, André; Thiry, Paul; Hanique, Sophie; Frère, Jean-Marie.

In: Langmuir, Vol. 20, No. 17, 2004, p. 7201-7207.

Research output: Contribution to journalArticle

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T1 - Electronic and molecular properties of an adsorbed protein monolayer probed by two-color sum-frequency generation spectroscopy

AU - Dreesen, Laurent

AU - Humbert, Christophe

AU - Sartenaer, Yannick

AU - Caudano, Yves

AU - Volcke, Cedric

AU - Mani, Alaa Addin

AU - Peremans, André

AU - Thiry, Paul

AU - Hanique, Sophie

AU - Frère, Jean-Marie

PY - 2004

Y1 - 2004

N2 - Two-color sum-frequency generation spectroscopy (2C-SFG) is used to probe the molecular and electronic properties of an adsorbed layer of the green fluorescent protein mutant 2 (GFPmut2) on a platinum (111) substrate. First, the spectroscopic measurements, performed under different polarization combinations, and atomic force microscopy (AFM) show that the GFPmut2 proteins form a fairly ordered monolayer on the platinum surface. Next, the nonlinear spectroscopic data provide evidence of particular coupling phenomena between the GFPmut2 vibrational and electronic properties. This is revealed by the occurrence of two doubly resonant sum-frequency generation processes for molecules having both their Raman and infrared transition moments in a direction perpendicular to the sample plane. Finally, our 2C-SFG analysis reveals two electronic transitions corresponding to the absorption and fluorescence energy levels which are related to two different GFPmut2 conformations: the B (anionic) and I forms, respectively. Their observation and wavelength positions attest the keeping of the GFPmut2 electronic properties upon adsorption on the metallic surface.

AB - Two-color sum-frequency generation spectroscopy (2C-SFG) is used to probe the molecular and electronic properties of an adsorbed layer of the green fluorescent protein mutant 2 (GFPmut2) on a platinum (111) substrate. First, the spectroscopic measurements, performed under different polarization combinations, and atomic force microscopy (AFM) show that the GFPmut2 proteins form a fairly ordered monolayer on the platinum surface. Next, the nonlinear spectroscopic data provide evidence of particular coupling phenomena between the GFPmut2 vibrational and electronic properties. This is revealed by the occurrence of two doubly resonant sum-frequency generation processes for molecules having both their Raman and infrared transition moments in a direction perpendicular to the sample plane. Finally, our 2C-SFG analysis reveals two electronic transitions corresponding to the absorption and fluorescence energy levels which are related to two different GFPmut2 conformations: the B (anionic) and I forms, respectively. Their observation and wavelength positions attest the keeping of the GFPmut2 electronic properties upon adsorption on the metallic surface.

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JO - Langmuir

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