TY - JOUR
T1 - Effects of Origanum majorana essential oil and antibiotics on the quality of frozen thawed Beni Arouss buck semen
AU - Kchikich, Amr
AU - Kirschvink, Nathalie
AU - El Kadili, Sara
AU - Raes, Marianne
AU - El Otmani, Samira
AU - Chebli, Youssef
AU - Bister, Jean Loup
AU - El Amiri, Bouchra
AU - Barrijal, Said
AU - Chentouf, Mouad
N1 - Funding Information:
This research was funded by the Academy for Research and Higher Education-Development Cooperation Committee (ARES-CCD), Brussels, Belgium, in the framework of the Research Project for Development (PRD: 2017-2022). The authors thank the staff of National Institute of Agricultural Research, Regional Center of Tangier for their support and collaboration.
Funding Information:
This research was funded by the Academy for Research and Higher Education‐Development Cooperation Committee (ARES‐CCD), Brussels, Belgium, in the framework of the Research Project for Development (PRD: 2017‐2022). The authors thank the staff of National Institute of Agricultural Research, Regional Center of Tangier for their support and collaboration.
Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2023/2
Y1 - 2023/2
N2 - This study aims to investigate the effects of Origanum majorana (OM) essential oil (EO) at different concentrations and antibiotics on post-thawed Beni Arouss buck semen quality. Semen collection was performed using artificial vagina from eight Beni Arouss bucks. Ejaculates were pooled, divided into 12 equal aliquots, washed and diluted to 400 × 106 sperm/ml (with 7% of glycerol). Skim milk–based extender was supplemented with different concentrations of OM EO (0%; 0.01%; 0.02%; 0.03%; 0.04% and 0.05%) without antibiotics, marked as (CTR−), (M1−), (M2−), (M3−), (M4−) and (M5−), and with 50 mg of streptomycin and 50,000 IU of penicillin per 100 ml, marked as (CTR+), (M1+), (M2+), (M3+), (M4+) and (M5+), respectively. Aliquots were cooled to 4°C, then frozen in 0.25 ml straws with a programmable freezer and finally stored in liquid nitrogen for 48 h. Thawing was performed at 37°C for 30 s. Motility, live sperm, sperm abnormalities, membrane integrity, lipid peroxidation and bacterial growth were evaluated after thawing. Among the tested extenders, M2+ improved all semen quality parameters. Sperm motility, live sperm and membrane integrity increased significantly, while the number of abnormal sperm and bacterial growth decreased significantly. The toxic effect of OM EO, with and without antibiotics, appeared beyond 0.03%. In conclusion, M2+ is recommended to improve the cryopreservation of Beni Arouss buck semen.
AB - This study aims to investigate the effects of Origanum majorana (OM) essential oil (EO) at different concentrations and antibiotics on post-thawed Beni Arouss buck semen quality. Semen collection was performed using artificial vagina from eight Beni Arouss bucks. Ejaculates were pooled, divided into 12 equal aliquots, washed and diluted to 400 × 106 sperm/ml (with 7% of glycerol). Skim milk–based extender was supplemented with different concentrations of OM EO (0%; 0.01%; 0.02%; 0.03%; 0.04% and 0.05%) without antibiotics, marked as (CTR−), (M1−), (M2−), (M3−), (M4−) and (M5−), and with 50 mg of streptomycin and 50,000 IU of penicillin per 100 ml, marked as (CTR+), (M1+), (M2+), (M3+), (M4+) and (M5+), respectively. Aliquots were cooled to 4°C, then frozen in 0.25 ml straws with a programmable freezer and finally stored in liquid nitrogen for 48 h. Thawing was performed at 37°C for 30 s. Motility, live sperm, sperm abnormalities, membrane integrity, lipid peroxidation and bacterial growth were evaluated after thawing. Among the tested extenders, M2+ improved all semen quality parameters. Sperm motility, live sperm and membrane integrity increased significantly, while the number of abnormal sperm and bacterial growth decreased significantly. The toxic effect of OM EO, with and without antibiotics, appeared beyond 0.03%. In conclusion, M2+ is recommended to improve the cryopreservation of Beni Arouss buck semen.
KW - Beni Arouss buck
KW - cryopreservation
KW - essential oil
KW - Origanum majorana
KW - semen quality
UR - http://www.scopus.com/inward/record.url?scp=85141355279&partnerID=8YFLogxK
U2 - 10.1111/rda.14285
DO - 10.1111/rda.14285
M3 - Article
AN - SCOPUS:85141355279
SN - 0936-6768
VL - 58
SP - 288
EP - 297
JO - Reproduction in Domestic Animals
JF - Reproduction in Domestic Animals
IS - 2
ER -