Effects of atrazine on osmeoregulation in the chinese mitten crab, Eriocheir sinensis

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Abstract

This study integrates results from acute contamination with atrazine of isolated perfused gills, and from in vivo chronic contamination of euryhaline Chinese mitten crabs, Eriocheir sinensis, acclimated to freshwater. Atrazine 1 mg/l in contact with the basolateral membrane (IN) increases the transepithelial potential difference (TEP) from -20.8+/-4.9 to -29.7+/-3.8 mV in isolated perfused posterior gills (P <0.01). This effect is only partially explained by a modification of Na+ and Cl- active influxes. No TEP modification is detected when atrazine is added (OUT) indicating that molecular mechanisms located on the basolateral membrane are likely to be the only ones affected. Another explanation would be that cuticular barrier prevents atrazine penetration into the gill. Haemolymph osmolarity, Na+ and Cl- concentrations of crabs living in freshwater. contaminated with atrazine 1 mg/l during 14 days are not significantly modified. We conclude that although atrazine can disturb osmoregulatory mechanisms of isolated gills, this pollutant would be of minor importance in affecting osmoregulatory capacities of the Chinese mitten crab in natural conditions. (C) 2002 Elsevier Science Inc. All rights reserved.
Original languageEnglish
Pages (from-to)385-390
Number of pages6
JournalComparative Biochemistry and Physiology Part C
Volume132
Issue number3
Publication statusPublished - 2002

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Atrazine
Fresh Water
Contamination
Membranes
Hemolymph
Osmolar Concentration

Keywords

  • Toxicology
  • Endocrinology & Metabolism
  • Zoology

Cite this

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title = "Effects of atrazine on osmeoregulation in the chinese mitten crab, Eriocheir sinensis",
abstract = "This study integrates results from acute contamination with atrazine of isolated perfused gills, and from in vivo chronic contamination of euryhaline Chinese mitten crabs, Eriocheir sinensis, acclimated to freshwater. Atrazine 1 mg/l in contact with the basolateral membrane (IN) increases the transepithelial potential difference (TEP) from -20.8+/-4.9 to -29.7+/-3.8 mV in isolated perfused posterior gills (P <0.01). This effect is only partially explained by a modification of Na+ and Cl- active influxes. No TEP modification is detected when atrazine is added (OUT) indicating that molecular mechanisms located on the basolateral membrane are likely to be the only ones affected. Another explanation would be that cuticular barrier prevents atrazine penetration into the gill. Haemolymph osmolarity, Na+ and Cl- concentrations of crabs living in freshwater. contaminated with atrazine 1 mg/l during 14 days are not significantly modified. We conclude that although atrazine can disturb osmoregulatory mechanisms of isolated gills, this pollutant would be of minor importance in affecting osmoregulatory capacities of the Chinese mitten crab in natural conditions. (C) 2002 Elsevier Science Inc. All rights reserved.",
keywords = "Toxicology, Endocrinology & Metabolism, Zoology",
author = "Frederic Silvestre and Gerard Trausch and Laura Spano and Pierre Devos",
year = "2002",
language = "English",
volume = "132",
pages = "385--390",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
issn = "1532-0456",
publisher = "Elsevier Inc.",
number = "3",

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TY - JOUR

T1 - Effects of atrazine on osmeoregulation in the chinese mitten crab, Eriocheir sinensis

AU - Silvestre, Frederic

AU - Trausch, Gerard

AU - Spano, Laura

AU - Devos, Pierre

PY - 2002

Y1 - 2002

N2 - This study integrates results from acute contamination with atrazine of isolated perfused gills, and from in vivo chronic contamination of euryhaline Chinese mitten crabs, Eriocheir sinensis, acclimated to freshwater. Atrazine 1 mg/l in contact with the basolateral membrane (IN) increases the transepithelial potential difference (TEP) from -20.8+/-4.9 to -29.7+/-3.8 mV in isolated perfused posterior gills (P <0.01). This effect is only partially explained by a modification of Na+ and Cl- active influxes. No TEP modification is detected when atrazine is added (OUT) indicating that molecular mechanisms located on the basolateral membrane are likely to be the only ones affected. Another explanation would be that cuticular barrier prevents atrazine penetration into the gill. Haemolymph osmolarity, Na+ and Cl- concentrations of crabs living in freshwater. contaminated with atrazine 1 mg/l during 14 days are not significantly modified. We conclude that although atrazine can disturb osmoregulatory mechanisms of isolated gills, this pollutant would be of minor importance in affecting osmoregulatory capacities of the Chinese mitten crab in natural conditions. (C) 2002 Elsevier Science Inc. All rights reserved.

AB - This study integrates results from acute contamination with atrazine of isolated perfused gills, and from in vivo chronic contamination of euryhaline Chinese mitten crabs, Eriocheir sinensis, acclimated to freshwater. Atrazine 1 mg/l in contact with the basolateral membrane (IN) increases the transepithelial potential difference (TEP) from -20.8+/-4.9 to -29.7+/-3.8 mV in isolated perfused posterior gills (P <0.01). This effect is only partially explained by a modification of Na+ and Cl- active influxes. No TEP modification is detected when atrazine is added (OUT) indicating that molecular mechanisms located on the basolateral membrane are likely to be the only ones affected. Another explanation would be that cuticular barrier prevents atrazine penetration into the gill. Haemolymph osmolarity, Na+ and Cl- concentrations of crabs living in freshwater. contaminated with atrazine 1 mg/l during 14 days are not significantly modified. We conclude that although atrazine can disturb osmoregulatory mechanisms of isolated gills, this pollutant would be of minor importance in affecting osmoregulatory capacities of the Chinese mitten crab in natural conditions. (C) 2002 Elsevier Science Inc. All rights reserved.

KW - Toxicology

KW - Endocrinology & Metabolism

KW - Zoology

M3 - Article

VL - 132

SP - 385

EP - 390

JO - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

JF - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

SN - 1532-0456

IS - 3

ER -