Effect of uridine dethiolation in the anticodon triplet of trna(Glu) on its association with trna(phe)

C. Houssier, P. Degée, K. Nicoghosian, H. Grosjean

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of U(34) dethiolation on the anticodon-anticodon association between E.coli tRNA(Glu) and yeast tRNA(Phe) has been studied by jhe temperature jump relaxation technique. An important destabilization upon replacement of the thioketo group of s2U(34) by a keto group, was revealed by a lowering of melting temperature of about 20° C. The measured kinetic parameters indicated that this destabilization effect was originated in an increase of dissociation and a decrease of association rate constants by a factor of 4 to 5. Modifications in both stacking interactions and flexibility in the anticodon loop would be responsible for this effect.

Original languageEnglish
Pages (from-to)1259-1266
Number of pages8
JournalJournal of biomolecular structure and dynamics
Volume5
Issue number6
DOIs
Publication statusPublished - 1 Jan 1988
Externally publishedYes

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