Effect of the phosphatidylcholine on the dynamical properties of the µ opioid receptor

Research output: Contribution to conferencePoster

Abstract

Opioid receptors, whose structures were revealed in
2012, are part of the G-protein coupled receptor
(GPCRs), target of 50 % of drugs on the market today
[1]. The structural and functional properties of
transmembrane proteins are affected by the lipid
environment [2]. For example, the hydrophobic
mismatch promotes dimerization by reducing the
entropy due to the adaptation of the membrane to
protein [3]. Dimerization of μ protein involves
different signaling pathways but are still poorly
understood.
To understand these processes, classical molecular dynamics (MD) simulations can provide numerous
clarifications. We modelize a coarse-grained patch of membrane with POPC (1-palmitoyl-2-oleoyl-snglycero-
3-phosphocholin) and the μ receptor by NAMD (NAnoscale Molecular Dynamic) for 1 μs.
Firstly, we examine the adaptation of the lipids to the receptor and vice versa. To explain such
adaptation, we examine precisely the interactions between the amino acids and lipids by calculating the
distance between each other and the tilt of the seven helices of the μ protein during the MD simulation.
Then, we study, via POCASA, the possible pockets on the protein to accomodate lipids and determine
differents key conformations through the clustering of the pockets. We further will use other lipids to
charaterize the different binding of the lipids on the μ receptor and show potential new conformations
of the μ receptor.
Ultimately, our goal is to perform MD simulations with the most realistic lipid composition, the
simplest and most significant in terms of protein/lipid interactions, in order to guide the docking
experiments and show the importance of the interaction of a protein with a lipid towards its structural
and functional properties.
Bibliography:
[1] Manglik A., Kruse A., Kobilka T., Thian F., Mathiesen J., Sunahara R., Pardo L., Weis W.,
Kobilka B., Granier S. (2012) Crystal structure of the mu-opioid receptor bound to a morphinan
antagonist Nature 485: 321-7
[2] Jastrzebska B., Debinski A., Filipek S., Palczewski K. (2011) Role of membrane integrity on G
protein-coupled receptors: rhodopsin stability and function Progress in Lipid Research 50: 267-77
[3] Soubias O., Niu S.-L., Mitchell D., Gawrisch K. (2008) Lipid-rhodopsin hydrophobic mismatch
alters rhodopsin helical content Journal of American Chemistry Society 130: 12465-71
Original languageEnglish
Publication statusPublished - 14 Nov 2014
Event8 th Annual meeting of the SFMBBM doctoral school - Université de Namur, Namur, Belgium
Duration: 14 Nov 201414 Nov 2014

Scientific committee

Scientific committee8 th Annual meeting of the SFMBBM doctoral school
CountryBelgium
CityNamur
Period14/11/1414/11/14

Projects

Equipment

  • Activities

    • 1 Participation in conference
    • 1 Participation in workshop, seminar, course

    8 th Annual meeting of the SFMBBM doctoral school

    Marie-Ange Angladon (Poster)

    14 Nov 2014

    Activity: Participating in or organising an event typesParticipation in conference

    Inititation à Python 3

    Marie-Ange Angladon (Participant)

    28 Jan 20141 Apr 2014

    Activity: Participating in or organising an event typesParticipation in workshop, seminar, course

    Cite this

    Angladon, M-A., Fossepre, M., Leherte, L., & Vercauteren, D. (2014). Effect of the phosphatidylcholine on the dynamical properties of the µ opioid receptor. Poster session presented at 8 th Annual meeting of the SFMBBM doctoral school, Namur, Belgium.