Abstract

Here, we show that key physicochemical parameters of commercial Silicon Carbide nanoparticles, such as the primary particles of about 53 nm in size, the agglomerates size, and the surface composition, are considerably modified with respect to the pristine conditions, during in vitro assessment. The use of sample conditioning stages, such as the pre-dispersion in aqueous media and the subsequent dispersion in a culture medium specific to the in vitro assay, produce modifications as the absorption of N, C, and O, from the culture medium, in the nanoparticles surface. Our results show that the sedimented dose, fraction of sedimented NPs during incubation and consequently in contact with cells seeded at the bottom, of Silicon Carbide nanoparticles can be measured from the particle size distribution obtained using a centrifugal liquid sedimentation technique. It is underlined that the variations observed in the physicochemical properties are related to the in vitro assay conditions. Culture medium and incubation time are found to influence the most the sedimented dose and consequently the cells dose uptake.
Original languageEnglish
Article number1875
Number of pages15
JournalJournal of Nanoparticle Research
Volume15
Issue number8
DOIs
Publication statusPublished - 1 Jan 2013

Fingerprint

culture media
Dispersions
Nanoparticles
Culture Media
Assays
Dose
Silicon carbide
silicon carbides
dosage
nanoparticles
Silicon
Sedimentation
Cell
conditioning
cells
particle size distribution
Particle Size
Conditioning
Surface structure
Particle size analysis

Keywords

  • SiC NPs
  • Size distribution
  • Dose assessment
  • In vitro
  • Sedimented dose

Cite this

@article{e531376aa9254da389a159e3b183cd93,
title = "Dose assessment of SiC nanoparticle dispersions during in vitro assays",
abstract = "Here, we show that key physicochemical parameters of commercial Silicon Carbide nanoparticles, such as the primary particles of about 53 nm in size, the agglomerates size, and the surface composition, are considerably modified with respect to the pristine conditions, during in vitro assessment. The use of sample conditioning stages, such as the pre-dispersion in aqueous media and the subsequent dispersion in a culture medium specific to the in vitro assay, produce modifications as the absorption of N, C, and O, from the culture medium, in the nanoparticles surface. Our results show that the sedimented dose, fraction of sedimented NPs during incubation and consequently in contact with cells seeded at the bottom, of Silicon Carbide nanoparticles can be measured from the particle size distribution obtained using a centrifugal liquid sedimentation technique. It is underlined that the variations observed in the physicochemical properties are related to the in vitro assay conditions. Culture medium and incubation time are found to influence the most the sedimented dose and consequently the cells dose uptake.",
keywords = "SiC NPs, Size distribution, Dose assessment, In vitro, Sedimented dose",
author = "{Mejia Mendoza}, {Jorge Humberto} and J.-P. Piret and F. No{\"e}l and B. Masereel and O. Toussaint and S. Lucas",
year = "2013",
month = "1",
day = "1",
doi = "10.1007/s11051-013-1875-z",
language = "English",
volume = "15",
journal = "Journal of Nanoparticle Research",
issn = "1388-0764",
publisher = "Springer Netherlands",
number = "8",

}

TY - JOUR

T1 - Dose assessment of SiC nanoparticle dispersions during in vitro assays

AU - Mejia Mendoza, Jorge Humberto

AU - Piret, J.-P.

AU - Noël, F.

AU - Masereel, B.

AU - Toussaint, O.

AU - Lucas, S.

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Here, we show that key physicochemical parameters of commercial Silicon Carbide nanoparticles, such as the primary particles of about 53 nm in size, the agglomerates size, and the surface composition, are considerably modified with respect to the pristine conditions, during in vitro assessment. The use of sample conditioning stages, such as the pre-dispersion in aqueous media and the subsequent dispersion in a culture medium specific to the in vitro assay, produce modifications as the absorption of N, C, and O, from the culture medium, in the nanoparticles surface. Our results show that the sedimented dose, fraction of sedimented NPs during incubation and consequently in contact with cells seeded at the bottom, of Silicon Carbide nanoparticles can be measured from the particle size distribution obtained using a centrifugal liquid sedimentation technique. It is underlined that the variations observed in the physicochemical properties are related to the in vitro assay conditions. Culture medium and incubation time are found to influence the most the sedimented dose and consequently the cells dose uptake.

AB - Here, we show that key physicochemical parameters of commercial Silicon Carbide nanoparticles, such as the primary particles of about 53 nm in size, the agglomerates size, and the surface composition, are considerably modified with respect to the pristine conditions, during in vitro assessment. The use of sample conditioning stages, such as the pre-dispersion in aqueous media and the subsequent dispersion in a culture medium specific to the in vitro assay, produce modifications as the absorption of N, C, and O, from the culture medium, in the nanoparticles surface. Our results show that the sedimented dose, fraction of sedimented NPs during incubation and consequently in contact with cells seeded at the bottom, of Silicon Carbide nanoparticles can be measured from the particle size distribution obtained using a centrifugal liquid sedimentation technique. It is underlined that the variations observed in the physicochemical properties are related to the in vitro assay conditions. Culture medium and incubation time are found to influence the most the sedimented dose and consequently the cells dose uptake.

KW - SiC NPs

KW - Size distribution

KW - Dose assessment

KW - In vitro

KW - Sedimented dose

UR - http://www.scopus.com/inward/record.url?scp=84883482219&partnerID=8YFLogxK

U2 - 10.1007/s11051-013-1875-z

DO - 10.1007/s11051-013-1875-z

M3 - Article

VL - 15

JO - Journal of Nanoparticle Research

JF - Journal of Nanoparticle Research

SN - 1388-0764

IS - 8

M1 - 1875

ER -