Distribution of pectic epitopes in cell walls of the sugar beet root

Florence Guillemin, Fabienne Guillon, Estelle Bonnin, Marie-Françoise Devaux, Thérèse Chevalier, J Paul Knox, Françoise Liners, Jean-François Thibault

Research output: Contribution to journalArticle

Abstract

Immunolabelling techniques with antibodies specific to partially methyl-esterified homogalacturonan (JIM5: unesterified residues flanked by methylesterified residues. JIM7: methyl-esterified residues flanked by unesterified residues), a blockwise de-esterified homogalacturonan (2F4), 1,4-galactan (LM5) and 1,5-arabinan (LM6) were used to map the distribution of pectin motifs in cell walls of sugar beet root (Beta vulgaris). PME and alkali treatments of sections were used in conjunction with JIM5-7 and 2F4. The JIM7 epitope was abundant and equally distributed in all cells. In storage parenchyma, the JIM5 epitope was restricted to some cell junctions and the lining of intercellular spaces while in vascular tissues it occurred at cell junctions in some phloem walls and in xylem derivatives. After secondary wall formation, the JIM5 epitope was restricted to inner cell wall regions between secondary thickenings. The 2F4 epitope was not detected without de-esterification treatment. PME treatments prior to the use of 2F4 indicated that HG at cell corners was not acetylated. The LM5 epitope was mainly present in the cambial zone and when present in storage parenchyma, it was restricted to the wall region closest to the plasma membrane. The LM6 epitope was widely distributed throughout primary walls but was more abundant in bundles than in medullar ray tissue and storage parenchyma. These data show that the occurrence of oligosaccharide motifs of pectic polysaccharides are spatially regulated in sugar beet root cell walls and that the spatial patterns vary between cell types suggesting that structural variants of pectic polymers are involved in the modulation of cell wall properties.
Original languageEnglish
Pages (from-to)355-71
Number of pages17
JournalPlanta
Volume222
Issue number2
DOIs
Publication statusPublished - Oct 2005

Fingerprint

Beta vulgaris
sugar beet
Cell Wall
epitopes
Epitopes
cell walls
intercellular junctions
parenchyma (plant tissue)
Intercellular Junctions
Galactans
Phloem
alkali treatment
Xylem
galactans
intercellular spaces
Esterification
Extracellular Space
Alkalies
vascular tissues
esterification

Keywords

  • Antibodies, Monoclonal
  • Plant Roots
  • Pectins
  • Cell Wall
  • Beta vulgaris
  • Polysaccharides
  • Galactans
  • Epitopes

Cite this

Guillemin, F., Guillon, F., Bonnin, E., Devaux, M-F., Chevalier, T., Knox, J. P., ... Thibault, J-F. (2005). Distribution of pectic epitopes in cell walls of the sugar beet root. Planta, 222(2), 355-71. https://doi.org/10.1007/s00425-005-1535-3
Guillemin, Florence ; Guillon, Fabienne ; Bonnin, Estelle ; Devaux, Marie-Françoise ; Chevalier, Thérèse ; Knox, J Paul ; Liners, Françoise ; Thibault, Jean-François. / Distribution of pectic epitopes in cell walls of the sugar beet root. In: Planta. 2005 ; Vol. 222, No. 2. pp. 355-71.
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Guillemin, F, Guillon, F, Bonnin, E, Devaux, M-F, Chevalier, T, Knox, JP, Liners, F & Thibault, J-F 2005, 'Distribution of pectic epitopes in cell walls of the sugar beet root', Planta, vol. 222, no. 2, pp. 355-71. https://doi.org/10.1007/s00425-005-1535-3

Distribution of pectic epitopes in cell walls of the sugar beet root. / Guillemin, Florence; Guillon, Fabienne; Bonnin, Estelle; Devaux, Marie-Françoise; Chevalier, Thérèse; Knox, J Paul; Liners, Françoise; Thibault, Jean-François.

In: Planta, Vol. 222, No. 2, 10.2005, p. 355-71.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Distribution of pectic epitopes in cell walls of the sugar beet root

AU - Guillemin, Florence

AU - Guillon, Fabienne

AU - Bonnin, Estelle

AU - Devaux, Marie-Françoise

AU - Chevalier, Thérèse

AU - Knox, J Paul

AU - Liners, Françoise

AU - Thibault, Jean-François

PY - 2005/10

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N2 - Immunolabelling techniques with antibodies specific to partially methyl-esterified homogalacturonan (JIM5: unesterified residues flanked by methylesterified residues. JIM7: methyl-esterified residues flanked by unesterified residues), a blockwise de-esterified homogalacturonan (2F4), 1,4-galactan (LM5) and 1,5-arabinan (LM6) were used to map the distribution of pectin motifs in cell walls of sugar beet root (Beta vulgaris). PME and alkali treatments of sections were used in conjunction with JIM5-7 and 2F4. The JIM7 epitope was abundant and equally distributed in all cells. In storage parenchyma, the JIM5 epitope was restricted to some cell junctions and the lining of intercellular spaces while in vascular tissues it occurred at cell junctions in some phloem walls and in xylem derivatives. After secondary wall formation, the JIM5 epitope was restricted to inner cell wall regions between secondary thickenings. The 2F4 epitope was not detected without de-esterification treatment. PME treatments prior to the use of 2F4 indicated that HG at cell corners was not acetylated. The LM5 epitope was mainly present in the cambial zone and when present in storage parenchyma, it was restricted to the wall region closest to the plasma membrane. The LM6 epitope was widely distributed throughout primary walls but was more abundant in bundles than in medullar ray tissue and storage parenchyma. These data show that the occurrence of oligosaccharide motifs of pectic polysaccharides are spatially regulated in sugar beet root cell walls and that the spatial patterns vary between cell types suggesting that structural variants of pectic polymers are involved in the modulation of cell wall properties.

AB - Immunolabelling techniques with antibodies specific to partially methyl-esterified homogalacturonan (JIM5: unesterified residues flanked by methylesterified residues. JIM7: methyl-esterified residues flanked by unesterified residues), a blockwise de-esterified homogalacturonan (2F4), 1,4-galactan (LM5) and 1,5-arabinan (LM6) were used to map the distribution of pectin motifs in cell walls of sugar beet root (Beta vulgaris). PME and alkali treatments of sections were used in conjunction with JIM5-7 and 2F4. The JIM7 epitope was abundant and equally distributed in all cells. In storage parenchyma, the JIM5 epitope was restricted to some cell junctions and the lining of intercellular spaces while in vascular tissues it occurred at cell junctions in some phloem walls and in xylem derivatives. After secondary wall formation, the JIM5 epitope was restricted to inner cell wall regions between secondary thickenings. The 2F4 epitope was not detected without de-esterification treatment. PME treatments prior to the use of 2F4 indicated that HG at cell corners was not acetylated. The LM5 epitope was mainly present in the cambial zone and when present in storage parenchyma, it was restricted to the wall region closest to the plasma membrane. The LM6 epitope was widely distributed throughout primary walls but was more abundant in bundles than in medullar ray tissue and storage parenchyma. These data show that the occurrence of oligosaccharide motifs of pectic polysaccharides are spatially regulated in sugar beet root cell walls and that the spatial patterns vary between cell types suggesting that structural variants of pectic polymers are involved in the modulation of cell wall properties.

KW - Antibodies, Monoclonal

KW - Plant Roots

KW - Pectins

KW - Cell Wall

KW - Beta vulgaris

KW - Polysaccharides

KW - Galactans

KW - Epitopes

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Guillemin F, Guillon F, Bonnin E, Devaux M-F, Chevalier T, Knox JP et al. Distribution of pectic epitopes in cell walls of the sugar beet root. Planta. 2005 Oct;222(2):355-71. https://doi.org/10.1007/s00425-005-1535-3