Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape

J.-P. Piret; S. Vankoningsloo; Jorge Humberto Mejia Mendoza; F. Noël; E. Boilan; F. Lambinon; C.C. Zouboulis; B. Masereel; S. Lucas; C. Saout; O. Toussaint

doi:10.3109/17435390.2011.625127

Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape

J.-P. Piret, S. Vankoningsloo, Jorge Humberto Mejia Mendoza, F. Noël, E. Boilan, F. Lambinon, C.C. Zouboulis, B. Masereel, S. Lucas, C. Saout, O. Toussaint

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Abstract

The potential toxic effects of copper oxide (CuO) nanoparticles (NPs) were studied on differentiated Caco-2 cell monolayers, a classical in vitro model of human small intestine epithelium. Two types of CuO NPs, with different specific surface area, different sizes as raw material but the same hydrodynamic diameter in suspension, differentially disturbed the monolayer integrity, were cytotoxic and triggered an increase of the abundance of several transcripts coding for pro-inflammatory cytokines and chemokines. Specific surface area was not a major variable explaining the increased toxicity when intestinal epithelium is exposed to rod-shaped CuO NPs, compared with spherical CuO NPs. The results suggest that release of Cu(II) cations and shape of these CuO NPs are likely to be implicated in the toxicity of these CuO NPs.

Original language	English
Pages (from-to)	789-803
Number of pages	15
Journal	Nanotoxicology
Volume	6
Issue number	7
DOIs	https://doi.org/10.3109/17435390.2011.625127
Publication status	Published - 1 Nov 2012

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10.3109/17435390.2011.625127

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Piret, J.-P., Vankoningsloo, S., Mejia Mendoza, J. H., Noël, F., Boilan, E., Lambinon, F., Zouboulis, C. C., Masereel, B., Lucas, S., Saout, C., & Toussaint, O. (2012). Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape. Nanotoxicology, 6(7), 789-803. https://doi.org/10.3109/17435390.2011.625127

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title = "Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape",

abstract = "The potential toxic effects of copper oxide (CuO) nanoparticles (NPs) were studied on differentiated Caco-2 cell monolayers, a classical in vitro model of human small intestine epithelium. Two types of CuO NPs, with different specific surface area, different sizes as raw material but the same hydrodynamic diameter in suspension, differentially disturbed the monolayer integrity, were cytotoxic and triggered an increase of the abundance of several transcripts coding for pro-inflammatory cytokines and chemokines. Specific surface area was not a major variable explaining the increased toxicity when intestinal epithelium is exposed to rod-shaped CuO NPs, compared with spherical CuO NPs. The results suggest that release of Cu(II) cations and shape of these CuO NPs are likely to be implicated in the toxicity of these CuO NPs.",

author = "J.-P. Piret and S. Vankoningsloo and {Mejia Mendoza}, {Jorge Humberto} and F. No{\"e}l and E. Boilan and F. Lambinon and C.C. Zouboulis and B. Masereel and S. Lucas and C. Saout and O. Toussaint",

year = "2012",

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doi = "10.3109/17435390.2011.625127",

language = "English",

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Piret, J-P, Vankoningsloo, S, Mejia Mendoza, JH, Noël, F, Boilan, E, Lambinon, F, Zouboulis, CC, Masereel, B , Lucas, S, Saout, C & Toussaint, O 2012, 'Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape', Nanotoxicology, vol. 6, no. 7, pp. 789-803. https://doi.org/10.3109/17435390.2011.625127

Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape. / Piret, J.-P.; Vankoningsloo, S.; Mejia Mendoza, Jorge Humberto et al.
In: Nanotoxicology, Vol. 6, No. 7, 01.11.2012, p. 789-803.

Research output: Contribution to journal › Article › peer-review

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T1 - Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape

AU - Piret, J.-P.

AU - Vankoningsloo, S.

AU - Mejia Mendoza, Jorge Humberto

AU - Noël, F.

AU - Boilan, E.

AU - Lambinon, F.

AU - Zouboulis, C.C.

AU - Masereel, B.

AU - Lucas, S.

AU - Saout, C.

AU - Toussaint, O.

PY - 2012/11/1

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AB - The potential toxic effects of copper oxide (CuO) nanoparticles (NPs) were studied on differentiated Caco-2 cell monolayers, a classical in vitro model of human small intestine epithelium. Two types of CuO NPs, with different specific surface area, different sizes as raw material but the same hydrodynamic diameter in suspension, differentially disturbed the monolayer integrity, were cytotoxic and triggered an increase of the abundance of several transcripts coding for pro-inflammatory cytokines and chemokines. Specific surface area was not a major variable explaining the increased toxicity when intestinal epithelium is exposed to rod-shaped CuO NPs, compared with spherical CuO NPs. The results suggest that release of Cu(II) cations and shape of these CuO NPs are likely to be implicated in the toxicity of these CuO NPs.

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Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape

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Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape

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Electron Microscopy

Light Microscopy

Synthesis, Irradiation and Analysis of Materials (SIAM)

Cite this