Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape

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Abstract

The potential toxic effects of copper oxide (CuO) nanoparticles (NPs) were studied on differentiated Caco-2 cell monolayers, a classical in vitro model of human small intestine epithelium. Two types of CuO NPs, with different specific surface area, different sizes as raw material but the same hydrodynamic diameter in suspension, differentially disturbed the monolayer integrity, were cytotoxic and triggered an increase of the abundance of several transcripts coding for pro-inflammatory cytokines and chemokines. Specific surface area was not a major variable explaining the increased toxicity when intestinal epithelium is exposed to rod-shaped CuO NPs, compared with spherical CuO NPs. The results suggest that release of Cu(II) cations and shape of these CuO NPs are likely to be implicated in the toxicity of these CuO NPs.
Original languageEnglish
Pages (from-to)789-803
Number of pages15
JournalNanotoxicology
Volume6
Issue number7
DOIs
Publication statusPublished - 1 Nov 2012

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Caco-2 Cells
Copper oxides
Hydrodynamics
Nanoparticles
Oxides
Toxicity
Copper
Monolayers
Specific surface area
Poisons
Intestinal Mucosa
Chemokines
Small Intestine
Cations
Suspensions
Raw materials
Epithelium
Positive ions
Cytokines

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@article{332b5e809a9e4442bb23a00c5be3bdcc,
title = "Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape",
abstract = "The potential toxic effects of copper oxide (CuO) nanoparticles (NPs) were studied on differentiated Caco-2 cell monolayers, a classical in vitro model of human small intestine epithelium. Two types of CuO NPs, with different specific surface area, different sizes as raw material but the same hydrodynamic diameter in suspension, differentially disturbed the monolayer integrity, were cytotoxic and triggered an increase of the abundance of several transcripts coding for pro-inflammatory cytokines and chemokines. Specific surface area was not a major variable explaining the increased toxicity when intestinal epithelium is exposed to rod-shaped CuO NPs, compared with spherical CuO NPs. The results suggest that release of Cu(II) cations and shape of these CuO NPs are likely to be implicated in the toxicity of these CuO NPs.",
author = "J.-P. Piret and S. Vankoningsloo and {Mejia Mendoza}, {Jorge Humberto} and F. No{\"e}l and E. Boilan and F. Lambinon and C.C. Zouboulis and B. Masereel and S. Lucas and C. Saout and O. Toussaint",
year = "2012",
month = "11",
day = "1",
doi = "10.3109/17435390.2011.625127",
language = "English",
volume = "6",
pages = "789--803",
journal = "Nanotoxicology",
issn = "1743-5390",
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TY - JOUR

T1 - Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape

AU - Piret, J.-P.

AU - Vankoningsloo, S.

AU - Mejia Mendoza, Jorge Humberto

AU - Noël, F.

AU - Boilan, E.

AU - Lambinon, F.

AU - Zouboulis, C.C.

AU - Masereel, B.

AU - Lucas, S.

AU - Saout, C.

AU - Toussaint, O.

PY - 2012/11/1

Y1 - 2012/11/1

N2 - The potential toxic effects of copper oxide (CuO) nanoparticles (NPs) were studied on differentiated Caco-2 cell monolayers, a classical in vitro model of human small intestine epithelium. Two types of CuO NPs, with different specific surface area, different sizes as raw material but the same hydrodynamic diameter in suspension, differentially disturbed the monolayer integrity, were cytotoxic and triggered an increase of the abundance of several transcripts coding for pro-inflammatory cytokines and chemokines. Specific surface area was not a major variable explaining the increased toxicity when intestinal epithelium is exposed to rod-shaped CuO NPs, compared with spherical CuO NPs. The results suggest that release of Cu(II) cations and shape of these CuO NPs are likely to be implicated in the toxicity of these CuO NPs.

AB - The potential toxic effects of copper oxide (CuO) nanoparticles (NPs) were studied on differentiated Caco-2 cell monolayers, a classical in vitro model of human small intestine epithelium. Two types of CuO NPs, with different specific surface area, different sizes as raw material but the same hydrodynamic diameter in suspension, differentially disturbed the monolayer integrity, were cytotoxic and triggered an increase of the abundance of several transcripts coding for pro-inflammatory cytokines and chemokines. Specific surface area was not a major variable explaining the increased toxicity when intestinal epithelium is exposed to rod-shaped CuO NPs, compared with spherical CuO NPs. The results suggest that release of Cu(II) cations and shape of these CuO NPs are likely to be implicated in the toxicity of these CuO NPs.

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U2 - 10.3109/17435390.2011.625127

DO - 10.3109/17435390.2011.625127

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EP - 803

JO - Nanotoxicology

JF - Nanotoxicology

SN - 1743-5390

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