Dietary Linseed Oil Reduces Growth while Differentially Impacting LC-PUFA Synthesis and Accretion into Tissues in Eurasian Perch (Perca fluviatilis)

F. Geay, D. Wenon, J. Mellery, E. Tinti, Robert Mandiki, D. R. Tocher, C. Debier, Y. Larondelle, P. Kestemont

Research output: Contribution to journalArticle

Abstract

The aim of this study was to evaluate the impact of replacing dietary fish oil (FO) with linseed oil (LO) on growth, fatty acid composition and regulation of lipid metabolism in Eurasian perch (Perca fluviatilis) juveniles. Fish (17.5 g initial body weight) were fed isoproteic and isoenergetic diets containing 116 g/kg of lipid for 10 weeks. Fish fed the LO diet displayed lower growth rates and lower levels of DHA in the liver and muscle than fish fed the FO diet, while mortality was not affected by dietary treatment. However, DHA content recorded in the liver and muscle of fish fed the LO diet remained relatively high, despite a weight gain of 134 % and a reduced dietary level of long-chain polyunsaturated fatty acids (LC-PUFA), suggesting endogenous LC-PUFA biosynthesis. This was supported by the higher amounts of pathway intermediates, including 18:4n-3, 20:3n-3, 20:4n-3, 18:3n-6 and 20:3n-6, recorded in the liver of fish fed the LO diet in comparison with those fed the FO diet. However, fads2 and elovl5 gene expression and FADS2 enzyme activity were comparable between the two groups. Similarly, the expression of genes involved in eicosanoid synthesis was not modulated by dietary LO. Thus, the present study demonstrated that in fish fed LO for 10 weeks, growth was reduced but DHA levels in tissues were largely maintained compared to fish fed FO, suggesting a physiologically relevant rate of endogenous LC-PUFA biosynthesis capacity.

Original languageEnglish
Pages (from-to)1219-1232
Number of pages14
JournalLipids
Volume50
Issue number12
DOIs
Publication statusPublished - 1 Dec 2015

Fingerprint

Linseed Oil
Perches
Unsaturated Dietary Fats
Unsaturated Fatty Acids
Fish
Nutrition
Fishes
Tissue
Fish Oils
Diet
Growth
Liver
Biosynthesis
Muscle
Gene Expression
Muscles
Eicosanoids
Enzyme activity
Lipid Metabolism
Gene expression

Keywords

  • Eicosanoid
  • Eurasian perch
  • FADS2
  • Fatty acid metabolism
  • LC-PUFA

Cite this

@article{405e6639c5914c71bec1ef57512e748f,
title = "Dietary Linseed Oil Reduces Growth while Differentially Impacting LC-PUFA Synthesis and Accretion into Tissues in Eurasian Perch (Perca fluviatilis)",
abstract = "The aim of this study was to evaluate the impact of replacing dietary fish oil (FO) with linseed oil (LO) on growth, fatty acid composition and regulation of lipid metabolism in Eurasian perch (Perca fluviatilis) juveniles. Fish (17.5 g initial body weight) were fed isoproteic and isoenergetic diets containing 116 g/kg of lipid for 10 weeks. Fish fed the LO diet displayed lower growth rates and lower levels of DHA in the liver and muscle than fish fed the FO diet, while mortality was not affected by dietary treatment. However, DHA content recorded in the liver and muscle of fish fed the LO diet remained relatively high, despite a weight gain of 134 {\%} and a reduced dietary level of long-chain polyunsaturated fatty acids (LC-PUFA), suggesting endogenous LC-PUFA biosynthesis. This was supported by the higher amounts of pathway intermediates, including 18:4n-3, 20:3n-3, 20:4n-3, 18:3n-6 and 20:3n-6, recorded in the liver of fish fed the LO diet in comparison with those fed the FO diet. However, fads2 and elovl5 gene expression and FADS2 enzyme activity were comparable between the two groups. Similarly, the expression of genes involved in eicosanoid synthesis was not modulated by dietary LO. Thus, the present study demonstrated that in fish fed LO for 10 weeks, growth was reduced but DHA levels in tissues were largely maintained compared to fish fed FO, suggesting a physiologically relevant rate of endogenous LC-PUFA biosynthesis capacity.",
keywords = "Eicosanoid, Eurasian perch, FADS2, Fatty acid metabolism, LC-PUFA",
author = "F. Geay and D. Wenon and J. Mellery and E. Tinti and Robert Mandiki and Tocher, {D. R.} and C. Debier and Y. Larondelle and P. Kestemont",
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language = "English",
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Dietary Linseed Oil Reduces Growth while Differentially Impacting LC-PUFA Synthesis and Accretion into Tissues in Eurasian Perch (Perca fluviatilis). / Geay, F.; Wenon, D.; Mellery, J.; Tinti, E.; Mandiki, Robert; Tocher, D. R.; Debier, C.; Larondelle, Y.; Kestemont, P.

In: Lipids, Vol. 50, No. 12, 01.12.2015, p. 1219-1232.

Research output: Contribution to journalArticle

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T1 - Dietary Linseed Oil Reduces Growth while Differentially Impacting LC-PUFA Synthesis and Accretion into Tissues in Eurasian Perch (Perca fluviatilis)

AU - Geay, F.

AU - Wenon, D.

AU - Mellery, J.

AU - Tinti, E.

AU - Mandiki, Robert

AU - Tocher, D. R.

AU - Debier, C.

AU - Larondelle, Y.

AU - Kestemont, P.

PY - 2015/12/1

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N2 - The aim of this study was to evaluate the impact of replacing dietary fish oil (FO) with linseed oil (LO) on growth, fatty acid composition and regulation of lipid metabolism in Eurasian perch (Perca fluviatilis) juveniles. Fish (17.5 g initial body weight) were fed isoproteic and isoenergetic diets containing 116 g/kg of lipid for 10 weeks. Fish fed the LO diet displayed lower growth rates and lower levels of DHA in the liver and muscle than fish fed the FO diet, while mortality was not affected by dietary treatment. However, DHA content recorded in the liver and muscle of fish fed the LO diet remained relatively high, despite a weight gain of 134 % and a reduced dietary level of long-chain polyunsaturated fatty acids (LC-PUFA), suggesting endogenous LC-PUFA biosynthesis. This was supported by the higher amounts of pathway intermediates, including 18:4n-3, 20:3n-3, 20:4n-3, 18:3n-6 and 20:3n-6, recorded in the liver of fish fed the LO diet in comparison with those fed the FO diet. However, fads2 and elovl5 gene expression and FADS2 enzyme activity were comparable between the two groups. Similarly, the expression of genes involved in eicosanoid synthesis was not modulated by dietary LO. Thus, the present study demonstrated that in fish fed LO for 10 weeks, growth was reduced but DHA levels in tissues were largely maintained compared to fish fed FO, suggesting a physiologically relevant rate of endogenous LC-PUFA biosynthesis capacity.

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