TY - JOUR
T1 - Dietary β-glucans differentially modulate immune and stress-related gene expression in lymphoid organs from healthy and Aeromonas hydrophila-infected rainbow trout (Oncorhynchus mykiss)
AU - Douxfils, Jessica
AU - Fierro-Castro, Camino
AU - Mandiki, Robert
AU - Emile, Wakson
AU - Tort, Lluis
AU - Kestemont, Patrick
N1 - Funding Information:
The study was carried out within the framework of the Interuniversity Attraction Poles (IAP) “AquaStress” project (P7/31) funded by the Belgian Science Policy Office. Biorigin company (Scandinavia) is warmly thanked for the free supply of Macrogard® and thanks are also due to A.Evrard for valuable technical support.
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Although β-glucans stimulating effects have already been demonstrated on the immune system of numerous animal species, available data remain relatively variable and more research should be done regarding the complexity of underlying mechanisms. In this context, the present study aimed to evaluate the stress and immune-related effects of dietary β-glucans (i.e. Macrogard®) by considering a number of influencing factors such as the dose (0, 0.1, 0.2 and 0.5% in food), feeding duration (15 versus 30 days), tissue (blood, kidney, spleen, gills) and infection status (healthy or infected). Blood parameters (lysozyme, ACH50 activities, leucocyte populations) and mRNA expression level of several immune- and stress-related genes (TFN-α1, IL-1β, IL10, COX-2, TGF-β, MC2R, HSP70) were measured. Our results suggest that spleen may be a highly responsive organ to dietary β-glucans both in healthy or infected fish, and that this organ may therefore significantly contribute to the immune reinforcement induced by such immunostimulatory diet. Our study further reveals that overdoses of β-glucans and/or prolonged medication can lead to a non-reactive physiological status and, consequently, to a poor immune response. All in all, the current data emphasizes the need for further extensive research in the field of dietary β-glucans as a preventive method for farmed fish protection.
AB - Although β-glucans stimulating effects have already been demonstrated on the immune system of numerous animal species, available data remain relatively variable and more research should be done regarding the complexity of underlying mechanisms. In this context, the present study aimed to evaluate the stress and immune-related effects of dietary β-glucans (i.e. Macrogard®) by considering a number of influencing factors such as the dose (0, 0.1, 0.2 and 0.5% in food), feeding duration (15 versus 30 days), tissue (blood, kidney, spleen, gills) and infection status (healthy or infected). Blood parameters (lysozyme, ACH50 activities, leucocyte populations) and mRNA expression level of several immune- and stress-related genes (TFN-α1, IL-1β, IL10, COX-2, TGF-β, MC2R, HSP70) were measured. Our results suggest that spleen may be a highly responsive organ to dietary β-glucans both in healthy or infected fish, and that this organ may therefore significantly contribute to the immune reinforcement induced by such immunostimulatory diet. Our study further reveals that overdoses of β-glucans and/or prolonged medication can lead to a non-reactive physiological status and, consequently, to a poor immune response. All in all, the current data emphasizes the need for further extensive research in the field of dietary β-glucans as a preventive method for farmed fish protection.
KW - Aeromonas
KW - Diet
KW - Gene expression
KW - Immunity
KW - Immunostimulant
KW - Infection
KW - Rainbow trout
KW - β-glucans
UR - http://www.scopus.com/inward/record.url?scp=85013996893&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2017.02.027
DO - 10.1016/j.fsi.2017.02.027
M3 - Article
AN - SCOPUS:85013996893
SN - 1050-4648
VL - 63
SP - 285
EP - 296
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
ER -