Development of novel microsatellite markers to identify the different invasive lineages in the Corbicula complex and to assess androgenesis

L-M Pigneur, A-M Risterucci, N Dauchot, X Li, K Van Doninck

Research output: Contribution to journalArticlepeer-review

Abstract

Reliable markers are needed to identify the lineages in the invasive clam genus Corbicula. Previous studies have demonstrated that mitochondrial (mt) DNA poorly resolves Corbicula phylogeny, owing to its androgenetic reproductive mode. Moreover, hybridization and mitochondrial/nuclear mismatches occur. We developed the first eleven polymorphic markers to detect these phenomena and to investigate the nuclear identity of Corbicula populations. These microsatellite loci revealed three main lineages in Western Europe. One locus allowed rapid discrimination of these three lineages on agarose gel, saving time and money. Moreover, the eleven markers were successfully cross-amplified in the invasive Corbicula lineages found in North America.
Original languageEnglish
Pages (from-to)573-7
Number of pages5
JournalMolecular Ecology Resources
Volume11
Issue number3
DOIs
Publication statusPublished - May 2011

Keywords

  • Phylogeny
  • Sexual Development
  • Polymerase Chain Reaction
  • Microsatellite Repeats
  • Animals
  • Polymorphism, Genetic
  • Molecular Sequence Data
  • Corbicula
  • Europe
  • Sequence Analysis, DNA
  • Time Factors

Fingerprint Dive into the research topics of 'Development of novel microsatellite markers to identify the different invasive lineages in the Corbicula complex and to assess androgenesis'. Together they form a unique fingerprint.

Cite this