Development, characterization, and biological assessment of biocompatible cellulosic wound dressing grafted Aloe vera bioactive polysaccharide

Fatma Salah; Yassine El Ghoul; Fahad M. Alminderej; Emna Golli; Zouhour Ouanes; Olek Maciejak; Nathalie Jarroux; Hatem Majdoub; Faouzi Sakli

doi:10.1007/s10570-019-02419-8

Development, characterization, and biological assessment of biocompatible cellulosic wound dressing grafted Aloe vera bioactive polysaccharide

Fatma Salah, Yassine El Ghoul, Fahad M. Alminderej, Emna Golli, Zouhour Ouanes, Olek Maciejak, Nathalie Jarroux, Hatem Majdoub, Faouzi Sakli

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract: In order to improve healthcare of injured people, deacetylated acemannan extracted from Aloe vera leaves, having high inhibitory properties, was used as an antimicrobial finish on traditional cotton items. Response surface methodology was employed to define quadratic relationships between the polysaccharide grafting degree and the treatment process properties. An optimized modification process, offering the highest funtionalization degree, is obtained. The cellulosic fiber morphology and roughness modifications induced by polymer grafting are revealed using Atomic Force Microscopy and Scanning Electron Microscopy. Infra Red spectroscopy was used to confirm the grafting effectiveness. Thermogravimetric Analysis and Differential Scanning Calorimeter were further employed to confirm chemical modification. Considering the potential use of this new biomaterial, original properties were also studied. Finishing treatment seems to preserve mechanical properties, and hydrophilicity of the cellulosic substrate. MTT assay were done in HepG2 cells to ensure that the obtained dressings are non-toxic. The biomaterial showed high biocompatibility and promoted cell viability. Antimicrobial studies showed that grafting treatment conserved polymer antibacterial activity. Optimized cotton dressings exhibited a significant inhibitory effect against Staphylococcus aureus and Escherichia coli bacteria, killed respectively at 70.2% and 72.4%. Graphical abstract: [Figure not available: see fulltext.].

Original language	English
Pages (from-to)	4957-4970
Number of pages	14
Journal	Cellulose
Volume	26
Issue number	8
DOIs	https://doi.org/10.1007/s10570-019-02419-8
Publication status	Published - 30 May 2019
Externally published	Yes

Funding

Acknowledgments The authors would like to acknowledge the University of Monastir, and, Tunisian Ministry of Higher Education and Scientific Research, for giving the opportunity and making easy, the achievement of this work. In addition, the authors thank the Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products in the Faculty of Pharmacy, for biological studies. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Keywords

Acemannan grafting
Antibacterial
Biocompatibility
Cotton
Dressings
Response surface methodology

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title = "Development, characterization, and biological assessment of biocompatible cellulosic wound dressing grafted Aloe vera bioactive polysaccharide",

abstract = "Abstract: In order to improve healthcare of injured people, deacetylated acemannan extracted from Aloe vera leaves, having high inhibitory properties, was used as an antimicrobial finish on traditional cotton items. Response surface methodology was employed to define quadratic relationships between the polysaccharide grafting degree and the treatment process properties. An optimized modification process, offering the highest funtionalization degree, is obtained. The cellulosic fiber morphology and roughness modifications induced by polymer grafting are revealed using Atomic Force Microscopy and Scanning Electron Microscopy. Infra Red spectroscopy was used to confirm the grafting effectiveness. Thermogravimetric Analysis and Differential Scanning Calorimeter were further employed to confirm chemical modification. Considering the potential use of this new biomaterial, original properties were also studied. Finishing treatment seems to preserve mechanical properties, and hydrophilicity of the cellulosic substrate. MTT assay were done in HepG2 cells to ensure that the obtained dressings are non-toxic. The biomaterial showed high biocompatibility and promoted cell viability. Antimicrobial studies showed that grafting treatment conserved polymer antibacterial activity. Optimized cotton dressings exhibited a significant inhibitory effect against Staphylococcus aureus and Escherichia coli bacteria, killed respectively at 70.2% and 72.4%. Graphical abstract: [Figure not available: see fulltext.].",

keywords = "Acemannan grafting, Antibacterial, Biocompatibility, Cotton, Dressings, Response surface methodology",

author = "Fatma Salah and {El Ghoul}, Yassine and Alminderej, {Fahad M.} and Emna Golli and Zouhour Ouanes and Olek Maciejak and Nathalie Jarroux and Hatem Majdoub and Faouzi Sakli",

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T1 - Development, characterization, and biological assessment of biocompatible cellulosic wound dressing grafted Aloe vera bioactive polysaccharide

AU - Salah, Fatma

AU - El Ghoul, Yassine

AU - Alminderej, Fahad M.

AU - Golli, Emna

AU - Ouanes, Zouhour

AU - Maciejak, Olek

AU - Jarroux, Nathalie

AU - Majdoub, Hatem

AU - Sakli, Faouzi

PY - 2019/5/30

Y1 - 2019/5/30

N2 - Abstract: In order to improve healthcare of injured people, deacetylated acemannan extracted from Aloe vera leaves, having high inhibitory properties, was used as an antimicrobial finish on traditional cotton items. Response surface methodology was employed to define quadratic relationships between the polysaccharide grafting degree and the treatment process properties. An optimized modification process, offering the highest funtionalization degree, is obtained. The cellulosic fiber morphology and roughness modifications induced by polymer grafting are revealed using Atomic Force Microscopy and Scanning Electron Microscopy. Infra Red spectroscopy was used to confirm the grafting effectiveness. Thermogravimetric Analysis and Differential Scanning Calorimeter were further employed to confirm chemical modification. Considering the potential use of this new biomaterial, original properties were also studied. Finishing treatment seems to preserve mechanical properties, and hydrophilicity of the cellulosic substrate. MTT assay were done in HepG2 cells to ensure that the obtained dressings are non-toxic. The biomaterial showed high biocompatibility and promoted cell viability. Antimicrobial studies showed that grafting treatment conserved polymer antibacterial activity. Optimized cotton dressings exhibited a significant inhibitory effect against Staphylococcus aureus and Escherichia coli bacteria, killed respectively at 70.2% and 72.4%. Graphical abstract: [Figure not available: see fulltext.].

AB - Abstract: In order to improve healthcare of injured people, deacetylated acemannan extracted from Aloe vera leaves, having high inhibitory properties, was used as an antimicrobial finish on traditional cotton items. Response surface methodology was employed to define quadratic relationships between the polysaccharide grafting degree and the treatment process properties. An optimized modification process, offering the highest funtionalization degree, is obtained. The cellulosic fiber morphology and roughness modifications induced by polymer grafting are revealed using Atomic Force Microscopy and Scanning Electron Microscopy. Infra Red spectroscopy was used to confirm the grafting effectiveness. Thermogravimetric Analysis and Differential Scanning Calorimeter were further employed to confirm chemical modification. Considering the potential use of this new biomaterial, original properties were also studied. Finishing treatment seems to preserve mechanical properties, and hydrophilicity of the cellulosic substrate. MTT assay were done in HepG2 cells to ensure that the obtained dressings are non-toxic. The biomaterial showed high biocompatibility and promoted cell viability. Antimicrobial studies showed that grafting treatment conserved polymer antibacterial activity. Optimized cotton dressings exhibited a significant inhibitory effect against Staphylococcus aureus and Escherichia coli bacteria, killed respectively at 70.2% and 72.4%. Graphical abstract: [Figure not available: see fulltext.].

KW - Acemannan grafting

KW - Antibacterial

KW - Biocompatibility

KW - Cotton

KW - Dressings

KW - Response surface methodology

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Development, characterization, and biological assessment of biocompatible cellulosic wound dressing grafted Aloe vera bioactive polysaccharide

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