TY - JOUR
T1 - Detecting envelope stress by monitoring β-barrel assembly
AU - Cho, Seung-Hyun
AU - Szewczyk, Joanna
AU - Pesavento, Christina
AU - Zietek, Matylda
AU - Banzhaf, Manuel
AU - Roszczenko, Paula
AU - Asmar, Abir
AU - Laloux, Géraldine
AU - Hov, Ann-Kristin
AU - Leverrier, Pauline
AU - Van der Henst, Charles
AU - Vertommen, Didier
AU - Typas, Athanasios
AU - Collet, Jean-François
N1 - Copyright © 2014 Elsevier Inc. All rights reserved.
PY - 2014
Y1 - 2014
N2 - The cell envelope protects bacteria from their surroundings. Defects in its integrity or assembly are sensed by signal transduction systems, allowing cells to rapidly adjust. The Rcs phosphorelay responds to outer membrane (OM)- and peptidoglycan-related stress in enterobacteria. We elucidated how the OM lipoprotein RcsF, the upstream Rcs component, senses envelope stress and activates the signaling cascade. RcsF interacts with BamA, the major component of the β-barrel assembly machinery. In growing cells, BamA continuously funnels RcsF through the β-barrel OmpA, displaying RcsF on the cell surface. This process spatially separates RcsF from the downstream Rcs component, which we show is the inner membrane protein IgaA. The Rcs system is activated when BamA fails to bind RcsF and funnel it to OmpA. Newly synthesized RcsF then remains periplasmic, interacting with IgaA to activate the cascade. Thus RcsF senses envelope damage by monitoring the activity of the Bam machinery.
AB - The cell envelope protects bacteria from their surroundings. Defects in its integrity or assembly are sensed by signal transduction systems, allowing cells to rapidly adjust. The Rcs phosphorelay responds to outer membrane (OM)- and peptidoglycan-related stress in enterobacteria. We elucidated how the OM lipoprotein RcsF, the upstream Rcs component, senses envelope stress and activates the signaling cascade. RcsF interacts with BamA, the major component of the β-barrel assembly machinery. In growing cells, BamA continuously funnels RcsF through the β-barrel OmpA, displaying RcsF on the cell surface. This process spatially separates RcsF from the downstream Rcs component, which we show is the inner membrane protein IgaA. The Rcs system is activated when BamA fails to bind RcsF and funnel it to OmpA. Newly synthesized RcsF then remains periplasmic, interacting with IgaA to activate the cascade. Thus RcsF senses envelope damage by monitoring the activity of the Bam machinery.
KW - Amino Acid Sequence
KW - Bacterial Outer Membrane Proteins
KW - Cell Membrane
KW - Cell Wall
KW - Escherichia coli
KW - Escherichia coli Proteins
KW - Membrane Proteins
KW - Molecular Sequence Data
KW - Protein Structure, Tertiary
KW - Sequence Alignment
KW - Signal Transduction
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.cell.2014.11.045
DO - 10.1016/j.cell.2014.11.045
M3 - Article
C2 - 25525882
SN - 1538-4101
VL - 159
SP - 1652
EP - 1664
JO - Cell Cycle
JF - Cell Cycle
IS - 7
ER -