Design of a synthetic adhesion protein by grafting RGD tailed cyclic peptides on bovine serum albumin

Dominique Delforge, Barbara Gillon, Muriel Art, Janique Dewelle, Martine Raes, José Remacle

Research output: Contribution to journalArticle

Abstract

A synthetic adhesion protein was designed by chemical grafting of the RGD tailed cyclic peptide cyclo[-D-Val-Arg-Gly-Asp-Glu(-∈Ahx-Tyr-Cys-NH2)-] on the carrier protein bovine serum albumin (BSA). The cyclic conformation of the RGD motif grafted on the protein mimics the conformation of the motif displayed in native adhesion proteins such as fibronectin. The adhesion of the cells on polystyrene coated with the conjugate BSA-peptide was similar or even better than the one obtained when the proadhesive protein fibronectin was coated on the plates. Results also indicated that covalent coupling of the peptide on BSA is not absolutely required, since simple adsorption of the peptide on the protein coated on plates was efficient for enhancing cell adhesion. These results show that polystyrene support can be reconditioned with conformationally constrained RGD peptides to enhance cell adhesion on solid supports. The same methodology can be adapted for the development of new biomaterials based on the recognition of specific peptides.

Original languageEnglish
Pages (from-to)87-91
Number of pages5
JournalLetters in peptide science
Volume5
Issue number2-3
Publication statusPublished - 1998

Fingerprint

Cyclic Peptides
Bovine Serum Albumin
Adhesion
Cell Adhesion
Peptides
Polystyrenes
Fibronectins
Cell adhesion
Proteins
Conformations
Protein Conformation
Biocompatible Materials
Adsorption
Carrier Proteins
Cells

Keywords

  • Biomaterials
  • Cell adhesion
  • Polystyrene
  • Protein carrier
  • Sulfo-SMCC

Cite this

Delforge, Dominique ; Gillon, Barbara ; Art, Muriel ; Dewelle, Janique ; Raes, Martine ; Remacle, José. / Design of a synthetic adhesion protein by grafting RGD tailed cyclic peptides on bovine serum albumin. In: Letters in peptide science. 1998 ; Vol. 5, No. 2-3. pp. 87-91.
@article{11504de74f194acc9d87123b49c4b053,
title = "Design of a synthetic adhesion protein by grafting RGD tailed cyclic peptides on bovine serum albumin",
abstract = "A synthetic adhesion protein was designed by chemical grafting of the RGD tailed cyclic peptide cyclo[-D-Val-Arg-Gly-Asp-Glu(-∈Ahx-Tyr-Cys-NH2)-] on the carrier protein bovine serum albumin (BSA). The cyclic conformation of the RGD motif grafted on the protein mimics the conformation of the motif displayed in native adhesion proteins such as fibronectin. The adhesion of the cells on polystyrene coated with the conjugate BSA-peptide was similar or even better than the one obtained when the proadhesive protein fibronectin was coated on the plates. Results also indicated that covalent coupling of the peptide on BSA is not absolutely required, since simple adsorption of the peptide on the protein coated on plates was efficient for enhancing cell adhesion. These results show that polystyrene support can be reconditioned with conformationally constrained RGD peptides to enhance cell adhesion on solid supports. The same methodology can be adapted for the development of new biomaterials based on the recognition of specific peptides.",
keywords = "Biomaterials, Cell adhesion, Polystyrene, Protein carrier, Sulfo-SMCC",
author = "Dominique Delforge and Barbara Gillon and Muriel Art and Janique Dewelle and Martine Raes and Jos{\'e} Remacle",
note = "Publication code : **RES. ACAD.",
year = "1998",
language = "English",
volume = "5",
pages = "87--91",
journal = "Letters in peptide science",
issn = "0929-5666",
publisher = "Kluwer Academic Publishers",
number = "2-3",

}

Design of a synthetic adhesion protein by grafting RGD tailed cyclic peptides on bovine serum albumin. / Delforge, Dominique; Gillon, Barbara; Art, Muriel; Dewelle, Janique; Raes, Martine; Remacle, José.

In: Letters in peptide science, Vol. 5, No. 2-3, 1998, p. 87-91.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Design of a synthetic adhesion protein by grafting RGD tailed cyclic peptides on bovine serum albumin

AU - Delforge, Dominique

AU - Gillon, Barbara

AU - Art, Muriel

AU - Dewelle, Janique

AU - Raes, Martine

AU - Remacle, José

N1 - Publication code : **RES. ACAD.

PY - 1998

Y1 - 1998

N2 - A synthetic adhesion protein was designed by chemical grafting of the RGD tailed cyclic peptide cyclo[-D-Val-Arg-Gly-Asp-Glu(-∈Ahx-Tyr-Cys-NH2)-] on the carrier protein bovine serum albumin (BSA). The cyclic conformation of the RGD motif grafted on the protein mimics the conformation of the motif displayed in native adhesion proteins such as fibronectin. The adhesion of the cells on polystyrene coated with the conjugate BSA-peptide was similar or even better than the one obtained when the proadhesive protein fibronectin was coated on the plates. Results also indicated that covalent coupling of the peptide on BSA is not absolutely required, since simple adsorption of the peptide on the protein coated on plates was efficient for enhancing cell adhesion. These results show that polystyrene support can be reconditioned with conformationally constrained RGD peptides to enhance cell adhesion on solid supports. The same methodology can be adapted for the development of new biomaterials based on the recognition of specific peptides.

AB - A synthetic adhesion protein was designed by chemical grafting of the RGD tailed cyclic peptide cyclo[-D-Val-Arg-Gly-Asp-Glu(-∈Ahx-Tyr-Cys-NH2)-] on the carrier protein bovine serum albumin (BSA). The cyclic conformation of the RGD motif grafted on the protein mimics the conformation of the motif displayed in native adhesion proteins such as fibronectin. The adhesion of the cells on polystyrene coated with the conjugate BSA-peptide was similar or even better than the one obtained when the proadhesive protein fibronectin was coated on the plates. Results also indicated that covalent coupling of the peptide on BSA is not absolutely required, since simple adsorption of the peptide on the protein coated on plates was efficient for enhancing cell adhesion. These results show that polystyrene support can be reconditioned with conformationally constrained RGD peptides to enhance cell adhesion on solid supports. The same methodology can be adapted for the development of new biomaterials based on the recognition of specific peptides.

KW - Biomaterials

KW - Cell adhesion

KW - Polystyrene

KW - Protein carrier

KW - Sulfo-SMCC

UR - http://www.scopus.com/inward/record.url?scp=0041893923&partnerID=8YFLogxK

M3 - Article

VL - 5

SP - 87

EP - 91

JO - Letters in peptide science

JF - Letters in peptide science

SN - 0929-5666

IS - 2-3

ER -