Cytotoxicity and related effects of T-2 toxin on cultured Vero cells

C. Bouaziz; S. Abid-Essefi; A. Bouslimi; Emna Golli; H. Bacha

doi:10.1016/j.toxicon.2006.06.004

Cytotoxicity and related effects of T-2 toxin on cultured Vero cells

C. Bouaziz, S. Abid-Essefi, A. Bouslimi, Emna Golli, H. Bacha

Research output: Contribution to journal › Article › peer-review

Abstract

T-2 toxin belongs to a group of mycotoxins synthesized by Fusarium fungi that are widely encountered as natural contaminants of certain important agricultural commodities particularly, cereals. Upon exposure, T-2 toxin causes severe human and animal diseases. It is considered to be a major causative agent in fatal alimentary toxic aleukia (ATA) in humans. In this study, cytotoxicity and apotosis induction by T-2 toxin was investigated in vitro on Vero cell line using the MTT and the neutral red viability assays, the induction of lipid peroxidation, the decrease of macromolecule levels (protein, DNA and RNA), DNA fragmentation and caspase-3-dependent apoptosis induction. Our results showed that T-2 toxin reduced cell viability correlated to an impairment of macromolecule levels. It also increased MDA formation, induced DNA fragmentation showed by DNA laddering patterns on agarose gel electrophoresis. This fragmentation is in relation with apoptosis induction which was confirmed by activation of caspase-3, and depletion of the mitochondrial membrane potential reflecting a mitochondrial dysfunction.

Original language	English
Pages (from-to)	343-352
Number of pages	10
Journal	Toxicon
Volume	48
Issue number	3
DOIs	https://doi.org/10.1016/j.toxicon.2006.06.004
Publication status	Published - 1 Sept 2006
Externally published	Yes

Funding

This research was supported by the (Ministère Tunisien de la Recherche Scientifique de la Technologie et du Développement des Compétences through the Laboratoire de Recherche sur les Substances Biologiquement Compatibles: LRSBC). A special thank for Dr. C. Lemaire and Dr. C. Brenner from LGBC, University of Versailles en Yvelines, France for their help. The authors are thankful to Mrs. Roxann Swesey for the revision of language.

Keywords

Apoptosis
Caspase-3
Cytotoxicity
DNA fragmentation
Lipid peroxidation
T-2 toxin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.toxicon.2006.06.004

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title = "Cytotoxicity and related effects of T-2 toxin on cultured Vero cells",

abstract = "T-2 toxin belongs to a group of mycotoxins synthesized by Fusarium fungi that are widely encountered as natural contaminants of certain important agricultural commodities particularly, cereals. Upon exposure, T-2 toxin causes severe human and animal diseases. It is considered to be a major causative agent in fatal alimentary toxic aleukia (ATA) in humans. In this study, cytotoxicity and apotosis induction by T-2 toxin was investigated in vitro on Vero cell line using the MTT and the neutral red viability assays, the induction of lipid peroxidation, the decrease of macromolecule levels (protein, DNA and RNA), DNA fragmentation and caspase-3-dependent apoptosis induction. Our results showed that T-2 toxin reduced cell viability correlated to an impairment of macromolecule levels. It also increased MDA formation, induced DNA fragmentation showed by DNA laddering patterns on agarose gel electrophoresis. This fragmentation is in relation with apoptosis induction which was confirmed by activation of caspase-3, and depletion of the mitochondrial membrane potential reflecting a mitochondrial dysfunction.",

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author = "C. Bouaziz and S. Abid-Essefi and A. Bouslimi and Emna Golli and H. Bacha",

note = "Funding Information: This research was supported by the (Minist{\`e}re Tunisien de la Recherche Scientifique de la Technologie et du D{\'e}veloppement des Comp{\'e}tences through the Laboratoire de Recherche sur les Substances Biologiquement Compatibles: LRSBC). A special thank for Dr. C. Lemaire and Dr. C. Brenner from LGBC, University of Versailles en Yvelines, France for their help. The authors are thankful to Mrs. Roxann Swesey for the revision of language.",

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AU - Bouaziz, C.

AU - Abid-Essefi, S.

AU - Bouslimi, A.

AU - Golli, Emna

AU - Bacha, H.

N1 - Funding Information: This research was supported by the (Ministère Tunisien de la Recherche Scientifique de la Technologie et du Développement des Compétences through the Laboratoire de Recherche sur les Substances Biologiquement Compatibles: LRSBC). A special thank for Dr. C. Lemaire and Dr. C. Brenner from LGBC, University of Versailles en Yvelines, France for their help. The authors are thankful to Mrs. Roxann Swesey for the revision of language.

PY - 2006/9/1

Y1 - 2006/9/1

N2 - T-2 toxin belongs to a group of mycotoxins synthesized by Fusarium fungi that are widely encountered as natural contaminants of certain important agricultural commodities particularly, cereals. Upon exposure, T-2 toxin causes severe human and animal diseases. It is considered to be a major causative agent in fatal alimentary toxic aleukia (ATA) in humans. In this study, cytotoxicity and apotosis induction by T-2 toxin was investigated in vitro on Vero cell line using the MTT and the neutral red viability assays, the induction of lipid peroxidation, the decrease of macromolecule levels (protein, DNA and RNA), DNA fragmentation and caspase-3-dependent apoptosis induction. Our results showed that T-2 toxin reduced cell viability correlated to an impairment of macromolecule levels. It also increased MDA formation, induced DNA fragmentation showed by DNA laddering patterns on agarose gel electrophoresis. This fragmentation is in relation with apoptosis induction which was confirmed by activation of caspase-3, and depletion of the mitochondrial membrane potential reflecting a mitochondrial dysfunction.

AB - T-2 toxin belongs to a group of mycotoxins synthesized by Fusarium fungi that are widely encountered as natural contaminants of certain important agricultural commodities particularly, cereals. Upon exposure, T-2 toxin causes severe human and animal diseases. It is considered to be a major causative agent in fatal alimentary toxic aleukia (ATA) in humans. In this study, cytotoxicity and apotosis induction by T-2 toxin was investigated in vitro on Vero cell line using the MTT and the neutral red viability assays, the induction of lipid peroxidation, the decrease of macromolecule levels (protein, DNA and RNA), DNA fragmentation and caspase-3-dependent apoptosis induction. Our results showed that T-2 toxin reduced cell viability correlated to an impairment of macromolecule levels. It also increased MDA formation, induced DNA fragmentation showed by DNA laddering patterns on agarose gel electrophoresis. This fragmentation is in relation with apoptosis induction which was confirmed by activation of caspase-3, and depletion of the mitochondrial membrane potential reflecting a mitochondrial dysfunction.

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KW - Caspase-3

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KW - Lipid peroxidation

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Cytotoxicity and related effects of T-2 toxin on cultured Vero cells

Abstract

Funding

Keywords

UN SDGs

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