Abstract
The potential toxic effects of two types of copper(ii) oxide (CuO) nanoparticles (NPs) with different specific surface areas, different shapes (rod or spheric), different sizes as raw materials and similar hydrodynamic diameter in suspension were studied on human hepatocarcinoma HepG2 cells. Both CuO NPs were shown to be able to enter into HepG2 cells and induce cellular toxicity by generating reactive oxygen species. CuO NPs increased the abundance of several transcripts coding for pro-inflammatory interleukins and chemokines. Transcriptomic data, siRNA knockdown and DNA binding activities suggested that Nrf2, NF-κB and AP-1 were implicated in the response of HepG2 cells to CuO NPs. CuO NP incubation also induced activation of MAPK pathways, ERKs and JNK/SAPK, playing a major role in the activation of AP-1. In addition, cytotoxicity, inflammatory and antioxidative responses and activation of intracellular transduction pathways induced by rod-shaped CuO NPs were more important than spherical CuO NPs. Measurement of Cu released in cell culture medium suggested that Cu cations released from CuO NPs were involved only to a small extent in the toxicity induced by these NPs on HepG2 cells.
Original language | English |
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Pages (from-to) | 7168-7184 |
Number of pages | 17 |
Journal | Nanoscale |
Volume | 4 |
Issue number | 22 |
DOIs | |
Publication status | Published - 21 Nov 2012 |
Keywords
- Chemokines
- Copper
- Heme Oxygenase-1
- Hep G2 Cells
- Humans
- Interleukin-8
- Interleukins
- MAP Kinase Signaling System
- Metal Nanoparticles
- Mitogen-Activated Protein Kinase Kinases
- NF-E2-Related Factor 2
- NF-kappa B
- Oxidative Stress
- RNA Interference
- RNA, Small Interfering
- Transcription Factor AP-1
Equipment
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Confocal microscope
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Equipment
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Scanning electron microscope
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Equipment
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Synthesis, Irradiation and Analysis of Materials (SIAM)
Pierre Louette (Manager), Julien Colaux (Manager), Alexandre Felten (Manager), Jorge Humberto Mejia Mendoza (Manager) & Paul-Louis Debarsy (Manager)
Technological Platform Synthesis, Irradiation and Analysis of MaterialsFacility/equipment: Technological Platform