Comparison of the linking arm effect on the biological performance of a CD31 agonist directly grafted on L605 CoCr alloy by a plasma-based multistep strategy

Sergio Diaz-Rodriguez, Caroline Loy, Pascale Chevallier, Céline Noël, Giuseppina Caligiuri, Laurent Houssiau, Diego Mantovani

Research output: Contribution to journalArticle

Abstract

Stents are cardiovascular implants deployed on atherosclerotic arteries that aid in reopening, sustaining, and avoiding their collapse. Nevertheless, postimplantation complications exist, and the risk of the renewal of the plaque subsists. Therefore, enhanced properties are mandatory requirements for clinics. For that purpose, a novel approach allowing the direct-grafting of bioactive molecules on cobalt-chromium devices (L605) has been developed. This original strategy involves the direct plasma functionalization of metallic surfaces with primary amines (-NH2). These groups act as anchor points to covalently graft biomolecules of interest, herein a peptide derived from CD31 (P23) with proendothelialization and antithrombotic properties. However, the biological activity of the grafted peptide could be impacted by its conformation. For this study, glutaric anhydride (GA), a short chain spacer, and polyethylene glycol (PEG) with antifouling properties were used as linking arms (LAs). The covalent grafting of the CD31 agonist on L605 by different LAs (GA-P23 and PEG-P23) was confirmed by XPS and ToF-SIMS analyses. The biological performance of these functionalized surfaces showed that, compared to the electropolished (EP) alloy, grafting the P23 with both LA increases adhesion and proliferation of endothelial cells (ECs) since day 1: EP = 68 ± 10%, GA-P23 = 101 ± 7%, and PEG-P23 = 106 ± 5% of cell viability. Moreover, ECs formed a complete monolayer at the surface, preventing clot formation (hemoglobin-free >80%). The potential of this plasma-based strategy for cardiovascular applications was confirmed by promoting a fast re-endothelialization, by improving the hemocompatibility of the alloy when coupled with the CD31 agonist and by its transfer onto commercial L605 stents, as confirmed by ToF-SIMS.

Original languageEnglish
Article number051009
Pages (from-to)051009
JournalBiointerphases
Volume14
Issue number5
DOIs
Publication statusPublished - 1 Sep 2019

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anhydrides
Polyethylene glycols
glycols
polyethylenes
Arm
Stents
Endothelial cells
Secondary ion mass spectrometry
activity (biology)
Plasmas
secondary ion mass spectrometry
Peptides
peptides
Endothelial Cells
antifouling
sustaining
Hemoglobin
hemoglobin
Biomolecules
Chromium

Cite this

Diaz-Rodriguez, Sergio ; Loy, Caroline ; Chevallier, Pascale ; Noël, Céline ; Caligiuri, Giuseppina ; Houssiau, Laurent ; Mantovani, Diego. / Comparison of the linking arm effect on the biological performance of a CD31 agonist directly grafted on L605 CoCr alloy by a plasma-based multistep strategy. In: Biointerphases. 2019 ; Vol. 14, No. 5. pp. 051009.
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Comparison of the linking arm effect on the biological performance of a CD31 agonist directly grafted on L605 CoCr alloy by a plasma-based multistep strategy. / Diaz-Rodriguez, Sergio; Loy, Caroline; Chevallier, Pascale; Noël, Céline; Caligiuri, Giuseppina; Houssiau, Laurent; Mantovani, Diego.

In: Biointerphases, Vol. 14, No. 5, 051009, 01.09.2019, p. 051009.

Research output: Contribution to journalArticle

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