Comparison of replicative senescence and stress-induced premature senescence combining differential display and low-density DNA arrays

Thierry Pascal; Florence Debacq-Chainiaux; Aline Chrétien; Coralie Bastin; Anne France Dabée; Vincent Bertholet; José Remacle; Olivier Toussaint

doi:10.1016/j.febslet.2005.05.056

Comparison of replicative senescence and stress-induced premature senescence combining differential display and low-density DNA arrays

Thierry Pascal, Florence Debacq-Chainiaux, Aline Chrétien, Coralie Bastin, Anne France Dabée, Vincent Bertholet, José Remacle, Olivier Toussaint

Research output: Contribution to journal › Article › peer-review

Abstract

Human diploid fibroblasts (HDFs) exposed to subcytotoxic stress display many features of senescence. Using differential display RT-PCR, gene expression of HDFs in premature senescence induced by tert-butylhydroperoxide or ethanol and in replicative senescence was compared to gene expression of HDFs at early cumulative population doublings. Thirty genes of known function were identified from the 265 differentially displayed cDNA fragments. A customized low-density array allowed to confirm the relative level of the corresponding 30 transcripts. We found differential expression of genes coding for proteins implicated namely in growth arrest (PTEN, IGFBP-3, LRP-1 and CAV1), senescent morphogenesis (TGF-β1 and LOXL2) and iron metabolism (TFR and FTL). © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	3651-3659
Number of pages	9
Journal	FEBS Letters
Volume	579
Issue number	17
DOIs	https://doi.org/10.1016/j.febslet.2005.05.056
Publication status	Published - 27 Jul 2005

Keywords

Ageing
Ethanol
Gene expression
Reactive oxygen species
Stress
TGF-β1

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10.1016/j.febslet.2005.05.056

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title = "Comparison of replicative senescence and stress-induced premature senescence combining differential display and low-density DNA arrays",

abstract = "Human diploid fibroblasts (HDFs) exposed to subcytotoxic stress display many features of senescence. Using differential display RT-PCR, gene expression of HDFs in premature senescence induced by tert-butylhydroperoxide or ethanol and in replicative senescence was compared to gene expression of HDFs at early cumulative population doublings. Thirty genes of known function were identified from the 265 differentially displayed cDNA fragments. A customized low-density array allowed to confirm the relative level of the corresponding 30 transcripts. We found differential expression of genes coding for proteins implicated namely in growth arrest (PTEN, IGFBP-3, LRP-1 and CAV1), senescent morphogenesis (TGF-β1 and LOXL2) and iron metabolism (TFR and FTL). {\textcopyright} 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",

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author = "Thierry Pascal and Florence Debacq-Chainiaux and Aline Chr{\'e}tien and Coralie Bastin and Dab{\'e}e, {Anne France} and Vincent Bertholet and Jos{\'e} Remacle and Olivier Toussaint",

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AU - Pascal, Thierry

AU - Debacq-Chainiaux, Florence

AU - Chrétien, Aline

AU - Bastin, Coralie

AU - Dabée, Anne France

AU - Bertholet, Vincent

AU - Remacle, José

AU - Toussaint, Olivier

PY - 2005/7/27

Y1 - 2005/7/27

N2 - Human diploid fibroblasts (HDFs) exposed to subcytotoxic stress display many features of senescence. Using differential display RT-PCR, gene expression of HDFs in premature senescence induced by tert-butylhydroperoxide or ethanol and in replicative senescence was compared to gene expression of HDFs at early cumulative population doublings. Thirty genes of known function were identified from the 265 differentially displayed cDNA fragments. A customized low-density array allowed to confirm the relative level of the corresponding 30 transcripts. We found differential expression of genes coding for proteins implicated namely in growth arrest (PTEN, IGFBP-3, LRP-1 and CAV1), senescent morphogenesis (TGF-β1 and LOXL2) and iron metabolism (TFR and FTL). © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

AB - Human diploid fibroblasts (HDFs) exposed to subcytotoxic stress display many features of senescence. Using differential display RT-PCR, gene expression of HDFs in premature senescence induced by tert-butylhydroperoxide or ethanol and in replicative senescence was compared to gene expression of HDFs at early cumulative population doublings. Thirty genes of known function were identified from the 265 differentially displayed cDNA fragments. A customized low-density array allowed to confirm the relative level of the corresponding 30 transcripts. We found differential expression of genes coding for proteins implicated namely in growth arrest (PTEN, IGFBP-3, LRP-1 and CAV1), senescent morphogenesis (TGF-β1 and LOXL2) and iron metabolism (TFR and FTL). © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

KW - Ageing

KW - Ethanol

KW - Gene expression

KW - Reactive oxygen species

KW - Stress

KW - TGF-β1

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DO - 10.1016/j.febslet.2005.05.056

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VL - 579

SP - 3651

EP - 3659

JO - FEBS Letters

JF - FEBS Letters

IS - 17

ER -

Comparison of replicative senescence and stress-induced premature senescence combining differential display and low-density DNA arrays

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Keywords

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