TY - JOUR
T1 - Comparative study of the enzymatic defense systems against oxygen-derived free radicals; the key role of glutathione peroxidase
AU - Raes, Martine
AU - Michiels, Carine
AU - Remacle, José
PY - 1987
Y1 - 1987
N2 - Human WI-38 diploid fibroblasts have been cultivated under high toxic O2 pressure, and their survival curves are reported. Superoxide dismutase, catalase, or glutathione peroxidase provided some protection when injected in the cells exposed to O2. This protective effect, recorded after 3 or 4 days of incubation, was the most pronounced when cells were injected just before oxygen exposure. Quantitative injection assays have been performed for the three enzymes. Surprisingly, glutathione peroxidase was found to be much more effective than both catalase and superoxide dismutase, the latter being particularly inefficient.
AB - Human WI-38 diploid fibroblasts have been cultivated under high toxic O2 pressure, and their survival curves are reported. Superoxide dismutase, catalase, or glutathione peroxidase provided some protection when injected in the cells exposed to O2. This protective effect, recorded after 3 or 4 days of incubation, was the most pronounced when cells were injected just before oxygen exposure. Quantitative injection assays have been performed for the three enzymes. Surprisingly, glutathione peroxidase was found to be much more effective than both catalase and superoxide dismutase, the latter being particularly inefficient.
KW - Catalase
KW - Glutathione peroxidase
KW - Microinjection
KW - Oxygen radicals
KW - Superoxide dismutase
UR - http://www.scopus.com/inward/record.url?scp=0023066133&partnerID=8YFLogxK
U2 - 10.1016/0891-5849(87)90032-3
DO - 10.1016/0891-5849(87)90032-3
M3 - Article
C2 - 3623187
AN - SCOPUS:0023066133
SN - 0891-5849
VL - 3
SP - 3
EP - 7
JO - Free Radical biology & medecine
JF - Free Radical biology & medecine
IS - 1
ER -