TY - JOUR
T1 - Close-packed array of gold nanoparticles and Sum Frequency Generation spectroscopy in total internal reflection: A platform for studying biomolecules and biosensors
AU - Tourillon, G.
AU - Dreesen, L.
AU - Volcke, C.
AU - Sartenaer, Y.
AU - Thiry, P.A.
AU - Peremans, A.
PY - 2009/1/1
Y1 - 2009/1/1
N2 - An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the sum frequency generation spectroscopy performed in the total internal reflection (TIR-SFG) geometry, combined with a regular close-packed array of gold nanoparticles allows to probe with a high sensitivity the changes in conformation and orientation induced by the recognition process of avidin by biocytin. This approach represents a new platform with potential use in biosensors, diagnostics and bioactive layers. © 2009 Springer Science+Business Media, LLC.
AB - An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the sum frequency generation spectroscopy performed in the total internal reflection (TIR-SFG) geometry, combined with a regular close-packed array of gold nanoparticles allows to probe with a high sensitivity the changes in conformation and orientation induced by the recognition process of avidin by biocytin. This approach represents a new platform with potential use in biosensors, diagnostics and bioactive layers. © 2009 Springer Science+Business Media, LLC.
KW - avidin.
KW - Biosensors
KW - sum frequency generation spectroscopy
KW - nanoparticles
KW - biocytin
UR - http://www.scopus.com/inward/record.url?scp=72049109436&partnerID=8YFLogxK
U2 - 10.1007/s10853-009-3572-y
DO - 10.1007/s10853-009-3572-y
M3 - Article
VL - 44
SP - 6805
EP - 6810
JO - Journal of Materials Science
JF - Journal of Materials Science
IS - 24
ER -