Close-packed array of gold nanoparticles and Sum Frequency Generation spectroscopy in total internal reflection: A platform for studying biomolecules and biosensors

G. Tourillon, L. Dreesen, C. Volcke, Y. Sartenaer, P.A. Thiry, A. Peremans

Research output: Contribution to journalArticle

Abstract

An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the sum frequency generation spectroscopy performed in the total internal reflection (TIR-SFG) geometry, combined with a regular close-packed array of gold nanoparticles allows to probe with a high sensitivity the changes in conformation and orientation induced by the recognition process of avidin by biocytin. This approach represents a new platform with potential use in biosensors, diagnostics and bioactive layers. © 2009 Springer Science+Business Media, LLC.
Original languageEnglish
Pages (from-to)6805-6810
Number of pages6
JournalJournal of Materials Science
Volume44
Issue number24
DOIs
Publication statusPublished - 1 Jan 2009

Fingerprint

Avidin
Biomolecules
Biosensors
Crystal orientation
Gold
Conformations
Spectroscopy
Nanoparticles
Adsorption
Geometry
Industry
biocytin

Keywords

  • avidin.
  • Biosensors
  • sum frequency generation spectroscopy
  • nanoparticles
  • biocytin

Cite this

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Close-packed array of gold nanoparticles and Sum Frequency Generation spectroscopy in total internal reflection: A platform for studying biomolecules and biosensors. / Tourillon, G.; Dreesen, L.; Volcke, C.; Sartenaer, Y.; Thiry, P.A.; Peremans, A.

In: Journal of Materials Science, Vol. 44, No. 24, 01.01.2009, p. 6805-6810.

Research output: Contribution to journalArticle

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