Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain

P A Denoel; M S Zygmunt; V Weynants; A Tibor; B Lichtfouse; Pascal Briffeuil; J N Limet; Jean-Jacques Letesson

Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain

P A Denoel, M S Zygmunt, V Weynants, A Tibor, B Lichtfouse, Pascal Briffeuil, J N Limet, Jean-Jacques Letesson

Namur Research Institute for Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melitensis HindIII-digested genomic DNA identified a 3.8 kb fragment. This fragment was shown to contain a bacterioferritin gene (bfr) encoding a 161-amino acid protein. The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli, and many of the ferroxidase centre and haem-ligation residues are conserved.

Original language	English
Pages (from-to)	238-42
Number of pages	5
Journal	FEBS Letters
Volume	361
Issue number	2-3
Publication status	Published - 1995

Biological Security Laboratory Level 3 (BL3)
De Bolle, X. (Manager)
Technological Platform: Biological Security Laboratory Level 3
Facility/equipment: Technological Platform

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title = "Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain",

abstract = "The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melitensis HindIII-digested genomic DNA identified a 3.8 kb fragment. This fragment was shown to contain a bacterioferritin gene (bfr) encoding a 161-amino acid protein. The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli, and many of the ferroxidase centre and haem-ligation residues are conserved.",

author = "Denoel, {P A} and Zygmunt, {M S} and V Weynants and A Tibor and B Lichtfouse and Pascal Briffeuil and Limet, {J N} and Jean-Jacques Letesson",

year = "1995",

language = "English",

volume = "361",

pages = "238--42",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "2-3",

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TY - JOUR

T1 - Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain

AU - Denoel, P A

AU - Zygmunt, M S

AU - Weynants, V

AU - Tibor, A

AU - Lichtfouse, B

AU - Briffeuil, Pascal

AU - Limet, J N

AU - Letesson, Jean-Jacques

PY - 1995

Y1 - 1995

N2 - The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melitensis HindIII-digested genomic DNA identified a 3.8 kb fragment. This fragment was shown to contain a bacterioferritin gene (bfr) encoding a 161-amino acid protein. The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli, and many of the ferroxidase centre and haem-ligation residues are conserved.

AB - The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melitensis HindIII-digested genomic DNA identified a 3.8 kb fragment. This fragment was shown to contain a bacterioferritin gene (bfr) encoding a 161-amino acid protein. The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli, and many of the ferroxidase centre and haem-ligation residues are conserved.

M3 - Article

C2 - 7698330

SN - 0014-5793

VL - 361

SP - 238

EP - 242

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain

Abstract

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Biological Security Laboratory Level 3 (BL3)

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