The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melitensis HindIII-digested genomic DNA identified a 3.8 kb fragment. This fragment was shown to contain a bacterioferritin gene (bfr) encoding a 161-amino acid protein. The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli, and many of the ferroxidase centre and haem-ligation residues are conserved.
|Number of pages||5|
|Publication status||Published - 1995|
Denoel, P. A., Zygmunt, M. S., Weynants, V., Tibor, A., Lichtfouse, B., Briffeuil, P., ... Letesson, J-J. (1995). Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain. FEBS Letters, 361(2-3), 238-42.