Click Reaction for Reversible Encapsulation of Single Yeast Cells

Wei Geng; Nan Jiang; Guang Yan Qing; Xiaolong Liu; Li Wang; Henk J. Busscher; Ge Tian; Taolei Sun; Li Wang; Yunuen Montelongo; Christoph Janiak; Guo Zhang; Xiao Yu Yang; Bao Lian Su

doi:10.1021/acsnano.9b08108

Click Reaction for Reversible Encapsulation of Single Yeast Cells

Wei Geng, Nan Jiang, Guang Yan Qing, Xiaolong Liu, Li Wang, Henk J. Busscher, Ge Tian, Taolei Sun, Li Wang, Yunuen Montelongo, Christoph Janiak, Guo Zhang, Xiao Yu Yang, Bao Lian Su

Unit of nanomaterials chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Cell surface engineering is an emerging technology to encapsulate cells in order to enhance their functions. However, methods for reversible encapsulation of cells with abiotic functionalities are rare. Herein, we describe a phenylboronic acid based click reaction for encapsulation of single yeast cells using mesoporous silica nanoparticles (MSNs). This encapsulation does not impact natural growth of the cells and leads to a significant enhancement of cell survival in a variety of hostile environments. Owing to the glucose-responsiveness of the boronate ester bond between cell surface polysaccharides and B(OH)₂-grafted MSNs, encapsulation was reversible by addition or removal of glucose. This effort offers living cells effective protection under harsh conditions and enables reversible assembling-detaching of abiotic functions.

Original language	English
Pages (from-to)	14459-14467
Number of pages	9
Journal	ACS nano
Volume	13
Issue number	12
DOIs	https://doi.org/10.1021/acsnano.9b08108
Publication status	Published - 24 Dec 2019

Keywords

cell protection
cell stability
click reaction
directed chemistry
hierarchical assembly
mesoporous materials
reversible encapsulation

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10.1021/acsnano.9b08108

Cite this

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title = "Click Reaction for Reversible Encapsulation of Single Yeast Cells",

abstract = "Cell surface engineering is an emerging technology to encapsulate cells in order to enhance their functions. However, methods for reversible encapsulation of cells with abiotic functionalities are rare. Herein, we describe a phenylboronic acid based click reaction for encapsulation of single yeast cells using mesoporous silica nanoparticles (MSNs). This encapsulation does not impact natural growth of the cells and leads to a significant enhancement of cell survival in a variety of hostile environments. Owing to the glucose-responsiveness of the boronate ester bond between cell surface polysaccharides and B(OH)2-grafted MSNs, encapsulation was reversible by addition or removal of glucose. This effort offers living cells effective protection under harsh conditions and enables reversible assembling-detaching of abiotic functions.",

keywords = "cell protection, cell stability, click reaction, directed chemistry, hierarchical assembly, mesoporous materials, reversible encapsulation",

author = "Wei Geng and Nan Jiang and Qing, {Guang Yan} and Xiaolong Liu and Li Wang and Busscher, {Henk J.} and Ge Tian and Taolei Sun and Li Wang and Yunuen Montelongo and Christoph Janiak and Guo Zhang and Yang, {Xiao Yu} and Su, {Bao Lian}",

note = "Funding Information: This work was supported by NSFC (51861135313, U1663225, U1662134, 21711530705, 21673282, 21473246), FRFCU (19lgpy112, 19lgzd16), National Key R&D Program of China (2017YFC1103800), PCSIRT (IRT_15R52), ISTCP (2015DFE52870), and JPSTDP (20180101208JC). We thank Prof. Fan Xia for QCM support at China University of Geosciences (Wuhan). H.J.B. is also director of the consulting company SASA BV. Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",

year = "2019",

month = dec,

day = "24",

doi = "10.1021/acsnano.9b08108",

language = "English",

volume = "13",

pages = "14459--14467",

journal = "ACS nano",

issn = "1936-0851",

publisher = "American Chemical Society",

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T1 - Click Reaction for Reversible Encapsulation of Single Yeast Cells

AU - Geng, Wei

AU - Jiang, Nan

AU - Qing, Guang Yan

AU - Liu, Xiaolong

AU - Wang, Li

AU - Busscher, Henk J.

AU - Tian, Ge

AU - Sun, Taolei

AU - Wang, Li

AU - Montelongo, Yunuen

AU - Janiak, Christoph

AU - Zhang, Guo

AU - Yang, Xiao Yu

AU - Su, Bao Lian

N1 - Funding Information: This work was supported by NSFC (51861135313, U1663225, U1662134, 21711530705, 21673282, 21473246), FRFCU (19lgpy112, 19lgzd16), National Key R&D Program of China (2017YFC1103800), PCSIRT (IRT_15R52), ISTCP (2015DFE52870), and JPSTDP (20180101208JC). We thank Prof. Fan Xia for QCM support at China University of Geosciences (Wuhan). H.J.B. is also director of the consulting company SASA BV. Publisher Copyright: © 2019 American Chemical Society.

PY - 2019/12/24

Y1 - 2019/12/24

N2 - Cell surface engineering is an emerging technology to encapsulate cells in order to enhance their functions. However, methods for reversible encapsulation of cells with abiotic functionalities are rare. Herein, we describe a phenylboronic acid based click reaction for encapsulation of single yeast cells using mesoporous silica nanoparticles (MSNs). This encapsulation does not impact natural growth of the cells and leads to a significant enhancement of cell survival in a variety of hostile environments. Owing to the glucose-responsiveness of the boronate ester bond between cell surface polysaccharides and B(OH)2-grafted MSNs, encapsulation was reversible by addition or removal of glucose. This effort offers living cells effective protection under harsh conditions and enables reversible assembling-detaching of abiotic functions.

AB - Cell surface engineering is an emerging technology to encapsulate cells in order to enhance their functions. However, methods for reversible encapsulation of cells with abiotic functionalities are rare. Herein, we describe a phenylboronic acid based click reaction for encapsulation of single yeast cells using mesoporous silica nanoparticles (MSNs). This encapsulation does not impact natural growth of the cells and leads to a significant enhancement of cell survival in a variety of hostile environments. Owing to the glucose-responsiveness of the boronate ester bond between cell surface polysaccharides and B(OH)2-grafted MSNs, encapsulation was reversible by addition or removal of glucose. This effort offers living cells effective protection under harsh conditions and enables reversible assembling-detaching of abiotic functions.

KW - cell protection

KW - cell stability

KW - click reaction

KW - directed chemistry

KW - hierarchical assembly

KW - mesoporous materials

KW - reversible encapsulation

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Click Reaction for Reversible Encapsulation of Single Yeast Cells

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Keywords

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Physical Chemistry and characterization(PC2)

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Click Reaction for Reversible Encapsulation of Single Yeast Cells

Abstract

Keywords

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Physical Chemistry and characterization(PC2)

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