TY - JOUR
T1 - Chronic effects of a binary insecticide Acer 35 EC on Nile tilapia Oreochromis niloticus through a multi-biomarker approach
AU - Guedegba, Nicresse Léa
AU - Imorou Toko, Ibrahim
AU - Ben Ammar, Imen
AU - François, Loïc
AU - Oreins, Noëlle
AU - Palluel, Olivier
AU - Mandiki, Syaghalirwa N.M.
AU - Jauniaux, Thierry
AU - Porcher, Jean Marc
AU - Scippo, Marie Louise
AU - Kestemont, Patrick
N1 - Funding Information:
The present study was supported by ARES-CCD (Académie de Recherche et d’Enseignement Supérieur-Commission de la Coopérative au Développement of Belgium) as part of the AQUATOX Bénin project. The authors are grateful to the Cell and Tissue Laboratory of the University of Namur (Belgium) and particularly to Daniel VAN VLAENDER and Benoît BALAU for their invaluable help in histological analyses. The authors express their thanks to Christelle Robert, Nathalie Gillard and Mathieu Dubois from BU laboratory, CER Marloie (Belgium) for the determination of the acetamiprid and lambda-cyhalothrin content in water and tissues. They also are thankful to the master degree students from the Department of Science and Technique of Animal Production of the University of Parakou (Benin) who participated in sampling and some analysis. Thanks to Carolin Mayer for the English editing of the manuscript.
Funding Information:
The present study was supported by ARES-CCD (Acad?mie de Recherche et d'Enseignement Sup?rieur-Commission de la Coop?rative au D?veloppement of Belgium) as part of the AQUATOX B?nin project. The authors are grateful to the Cell and Tissue Laboratory of the University of Namur (Belgium) and particularly to Daniel VAN VLAENDER and Beno?t BALAU for their invaluable help in histological analyses. The authors express their thanks to Christelle Robert, Nathalie Gillard and Mathieu Dubois from BU laboratory, CER Marloie (Belgium) for the determination of the acetamiprid and lambda-cyhalothrin content in water and tissues. They also are thankful to the master degree students from the Department of Science and Technique of Animal Production of the University of Parakou (Benin) who participated in sampling and some analysis. Thanks to Carolin Mayer for the English editing of the manuscript.
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2021/6
Y1 - 2021/6
N2 - Acer 35 EC is a widely used insecticide (a binary mixture of lambda-cyhalothrin and acetamiprid) in pest control in many West African countries, particularly in the cotton culture in north Benin. The aim of this study was to investigate the chronic effects of Acer 35 EC on Nile tilapia Oreochromis niloticus juveniles using a multi-biomarker approach under laboratory conditions. For this purpose, fish were exposed to sublethal concentrations of Acer 35 EC (0, 1 and 10% of LC50- 96 h value). After 28 and 56 days of exposure, several biomarkers were measured in males and females including enzymatic activities related to detoxification and oxidative stress, neurotoxicity and immune responses, sex steroid hormones (testosterone, 17β-estradiol and 11-keto-testosterone) and histological alterations of liver, kidney and gonads. An Integrated Biomarker Response (IBR) was then calculated. The results showed a reduction of cholinesterase activity in muscles, and intercellular superoxide anion production in both sexes. Female steroidogenesis and gametogenesis were affected, especially testosterone levels and oocyte growth. More alterations were observed in liver after exposure to Acer 35 EC. In both sexes, IBR values were higher after 56 days than after 28 days of exposure. In conclusion, based on a large set of biomarkers and IBR values, the chronic exposure to low doses of insecticide Acer 35 EC seems to impair different physiological functions in Nile tilapia juveniles on a time-dependent manner, with a stronger impact on females than on males.
AB - Acer 35 EC is a widely used insecticide (a binary mixture of lambda-cyhalothrin and acetamiprid) in pest control in many West African countries, particularly in the cotton culture in north Benin. The aim of this study was to investigate the chronic effects of Acer 35 EC on Nile tilapia Oreochromis niloticus juveniles using a multi-biomarker approach under laboratory conditions. For this purpose, fish were exposed to sublethal concentrations of Acer 35 EC (0, 1 and 10% of LC50- 96 h value). After 28 and 56 days of exposure, several biomarkers were measured in males and females including enzymatic activities related to detoxification and oxidative stress, neurotoxicity and immune responses, sex steroid hormones (testosterone, 17β-estradiol and 11-keto-testosterone) and histological alterations of liver, kidney and gonads. An Integrated Biomarker Response (IBR) was then calculated. The results showed a reduction of cholinesterase activity in muscles, and intercellular superoxide anion production in both sexes. Female steroidogenesis and gametogenesis were affected, especially testosterone levels and oocyte growth. More alterations were observed in liver after exposure to Acer 35 EC. In both sexes, IBR values were higher after 56 days than after 28 days of exposure. In conclusion, based on a large set of biomarkers and IBR values, the chronic exposure to low doses of insecticide Acer 35 EC seems to impair different physiological functions in Nile tilapia juveniles on a time-dependent manner, with a stronger impact on females than on males.
KW - Acetamiprid
KW - Biomarkers
KW - Chronic effects
KW - Integrated biomarker response
KW - Lambda-cyhalothrin
KW - Nile tilapia
UR - http://www.scopus.com/inward/record.url?scp=85093923578&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2020.128530
DO - 10.1016/j.chemosphere.2020.128530
M3 - Article
C2 - 33268085
AN - SCOPUS:85093923578
SN - 0045-6535
VL - 273
JO - Chemosphere
JF - Chemosphere
M1 - 128530
ER -
