TY - JOUR
T1 - Chromosome-level genome assembly reveals homologous chromosomes and recombination in asexual rotifer Adineta vaga
AU - Simion, Paul
AU - Narayan, Jitendra
AU - Houtain, Antoine
AU - Derzelle, Alessandro
AU - Baudry, Lyam
AU - Nicolas, Emilien
AU - Arora, Rohan
AU - Cariou, Marie
AU - Cruaud, Corinne
AU - Gaudray, Florence Rodriguez
AU - Gilbert, Clément
AU - Guiglielmoni, Nadège
AU - Hespeels, Boris
AU - Kozlowski, Djampa K.L.
AU - Labadie, Karine
AU - Limasset, Antoine
AU - Llirós, Marc
AU - Marbouty, Martial
AU - Terwagne, Matthieu
AU - Virgo, Julie
AU - Cordaux, Richard
AU - Danchin, Etienne G.J.
AU - Hallet, Bernard
AU - Koszul, Romain
AU - Lenormand, Thomas
AU - Flot, Jean Francois
AU - Van Doninck, Karine
N1 - Publisher Copyright:
© 2021 The Authors.
PY - 2021/10
Y1 - 2021/10
N2 - Bdelloid rotifers are notorious as a speciose ancient clade comprising only asexual lineages. Thanks to their ability to repair highly fragmented DNA, most bdelloid species also withstand complete desiccation and ionizing radiation. Producing a well-assembled reference genome is a critical step to developing an understanding of the effects of long-term asexuality and DNA breakage on genome evolution. To this end, we present the first high-quality chromosome-level genome assemblies for the bdelloid Adineta vaga, composed of six pairs of homologous (diploid) chromosomes with a footprint of paleotetraploidy. The observed large-scale losses of heterozygosity are signatures of recombination between homologous chromosomes, either during mitotic DNA double-strand break repair or when resolving programmed DNA breaks during a modified meiosis. Dynamic subtelomeric regions harbor more structural diversity (e.g., chromosome rearrangements, transposable elements, and haplotypic divergence). Our results trigger the reappraisal of potential meiotic processes in bdelloid rotifers and help unravel the factors underlying their long-term asexual evolutionary success.
AB - Bdelloid rotifers are notorious as a speciose ancient clade comprising only asexual lineages. Thanks to their ability to repair highly fragmented DNA, most bdelloid species also withstand complete desiccation and ionizing radiation. Producing a well-assembled reference genome is a critical step to developing an understanding of the effects of long-term asexuality and DNA breakage on genome evolution. To this end, we present the first high-quality chromosome-level genome assemblies for the bdelloid Adineta vaga, composed of six pairs of homologous (diploid) chromosomes with a footprint of paleotetraploidy. The observed large-scale losses of heterozygosity are signatures of recombination between homologous chromosomes, either during mitotic DNA double-strand break repair or when resolving programmed DNA breaks during a modified meiosis. Dynamic subtelomeric regions harbor more structural diversity (e.g., chromosome rearrangements, transposable elements, and haplotypic divergence). Our results trigger the reappraisal of potential meiotic processes in bdelloid rotifers and help unravel the factors underlying their long-term asexual evolutionary success.
UR - http://www.scopus.com/inward/record.url?scp=85116637904&partnerID=8YFLogxK
U2 - 10.1126/sciadv.abg4216
DO - 10.1126/sciadv.abg4216
M3 - Article
C2 - 34613768
AN - SCOPUS:85116637904
SN - 2375-2548
VL - 7
JO - Science Advances
JF - Science Advances
IS - 41
M1 - eabg4216
ER -