Characterization of polyethylene glycol self-assembled monolayers by means of sum-frequency generation spectroscopy for biosensor applications

Nadia Peerboom, F. Cecchet, Y. Caudano, J. Moreau, S. Wautier, J. Marchand-Brynaert, M. Henry, S. Demoustier-Champagne, D. Lis

Research output: Contribution to journalArticle

Abstract

Protein biochips are miniaturized biological sensors intended to analyze and characterize biomolecule interactions with high throughput. An important issue when developing such biochips is substrate passivation. The support is rendered inert in order to avoid non-specific adsorption. Strategic control of the non-specific protein adsorption can be achieved by creating a resistant self-assembled monolayer (SAM) based on polyethylene glycol (PEG). The degree of resistance depends on the PEG surface density, i.e. the number of PEG units the molecule contains. Infrared-visible sum-frequency generation (SFG) spectroscopy (Lambert et al., Appl Spectrosc Rev 40:103–145, 2005) is used to in-vestigate the vibrational fingerprint of a PEG self-assembled monolayer adsorbed on a flat platinum surface, in the 2,750–3,050 cm-1 frequency range. The objec-tive is to characterize the SFG baseline of the biosensor that will be further developed by mixing the PEG antifouling layer with bioactive host molecules. Nanostructures will then be implemented on the substrate in order to enhance the SFG signal through localized surface plasmon resonances (Lis et al., Adv Opt Mater 1:244–255, 2013). The ultimate goal will be to detect the SFG signature of the antigen/antibody recognition process at the interface of the above biosensing layer. The general chemical formula of the molecule investigated is C3H5S2 -(CH2)4 –C= O-NH-(CH2 -CH2 -O)n -CH3. The molecule studied here holds 7 polyethylene glycol units (n= 7). Former studies carried out by quartz crystal microbalance (QCM) and electrochemistry showed that molecules containing 7 PEG chains have the best antifouling properties. The SFG spectra present several vibrational modes (Sartenaer et al., Biosens Bioelectron 22:2179–2183, 2007; Cimatu et al., J Phys Chem C 112:14529–14537, 2008; Even et al., Macromolecules 39:9396–9401, 2006). The most significant contributions come from the CH2, O-CH2 and O-CH3 modes.

Original languageEnglish
Pages (from-to)545-546
Number of pages2
JournalNATO Science for Peace and Security Series B: Physics and Biophysics
Volume68
DOIs
Publication statusPublished - 1 Jan 2015

Fingerprint

Biosensing Techniques
Self assembled monolayers
bioinstrumentation
Biosensors
Polyethylene glycols
glycols
polyethylenes
Spectrum Analysis
Spectroscopy
spectroscopy
Molecules
antifouling
Biochips
molecules
Adsorption
Quartz Crystal Microbalance Techniques
proteins
Proteins
Electrochemistry
Protein Array Analysis

Cite this

@article{57aea29c15774f869ccb64453f65170e,
title = "Characterization of polyethylene glycol self-assembled monolayers by means of sum-frequency generation spectroscopy for biosensor applications",
abstract = "Protein biochips are miniaturized biological sensors intended to analyze and characterize biomolecule interactions with high throughput. An important issue when developing such biochips is substrate passivation. The support is rendered inert in order to avoid non-specific adsorption. Strategic control of the non-specific protein adsorption can be achieved by creating a resistant self-assembled monolayer (SAM) based on polyethylene glycol (PEG). The degree of resistance depends on the PEG surface density, i.e. the number of PEG units the molecule contains. Infrared-visible sum-frequency generation (SFG) spectroscopy (Lambert et al., Appl Spectrosc Rev 40:103–145, 2005) is used to in-vestigate the vibrational fingerprint of a PEG self-assembled monolayer adsorbed on a flat platinum surface, in the 2,750–3,050 cm-1 frequency range. The objec-tive is to characterize the SFG baseline of the biosensor that will be further developed by mixing the PEG antifouling layer with bioactive host molecules. Nanostructures will then be implemented on the substrate in order to enhance the SFG signal through localized surface plasmon resonances (Lis et al., Adv Opt Mater 1:244–255, 2013). The ultimate goal will be to detect the SFG signature of the antigen/antibody recognition process at the interface of the above biosensing layer. The general chemical formula of the molecule investigated is C3H5S2 -(CH2)4 –C= O-NH-(CH2 -CH2 -O)n -CH3. The molecule studied here holds 7 polyethylene glycol units (n= 7). Former studies carried out by quartz crystal microbalance (QCM) and electrochemistry showed that molecules containing 7 PEG chains have the best antifouling properties. The SFG spectra present several vibrational modes (Sartenaer et al., Biosens Bioelectron 22:2179–2183, 2007; Cimatu et al., J Phys Chem C 112:14529–14537, 2008; Even et al., Macromolecules 39:9396–9401, 2006). The most significant contributions come from the CH2, O-CH2 and O-CH3 modes.",
author = "Nadia Peerboom and F. Cecchet and Y. Caudano and J. Moreau and S. Wautier and J. Marchand-Brynaert and M. Henry and S. Demoustier-Champagne and D. Lis",
year = "2015",
month = "1",
day = "1",
doi = "10.1007/978-94-017-9133-5_62",
language = "English",
volume = "68",
pages = "545--546",
journal = "NATO Science for Peace and Security Series B: Physics and Biophysics",
issn = "1874-6500",
publisher = "Springer Verlag",

}

Characterization of polyethylene glycol self-assembled monolayers by means of sum-frequency generation spectroscopy for biosensor applications. / Peerboom, Nadia; Cecchet, F.; Caudano, Y.; Moreau, J.; Wautier, S.; Marchand-Brynaert, J.; Henry, M.; Demoustier-Champagne, S.; Lis, D.

In: NATO Science for Peace and Security Series B: Physics and Biophysics, Vol. 68, 01.01.2015, p. 545-546.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of polyethylene glycol self-assembled monolayers by means of sum-frequency generation spectroscopy for biosensor applications

AU - Peerboom, Nadia

AU - Cecchet, F.

AU - Caudano, Y.

AU - Moreau, J.

AU - Wautier, S.

AU - Marchand-Brynaert, J.

AU - Henry, M.

AU - Demoustier-Champagne, S.

AU - Lis, D.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Protein biochips are miniaturized biological sensors intended to analyze and characterize biomolecule interactions with high throughput. An important issue when developing such biochips is substrate passivation. The support is rendered inert in order to avoid non-specific adsorption. Strategic control of the non-specific protein adsorption can be achieved by creating a resistant self-assembled monolayer (SAM) based on polyethylene glycol (PEG). The degree of resistance depends on the PEG surface density, i.e. the number of PEG units the molecule contains. Infrared-visible sum-frequency generation (SFG) spectroscopy (Lambert et al., Appl Spectrosc Rev 40:103–145, 2005) is used to in-vestigate the vibrational fingerprint of a PEG self-assembled monolayer adsorbed on a flat platinum surface, in the 2,750–3,050 cm-1 frequency range. The objec-tive is to characterize the SFG baseline of the biosensor that will be further developed by mixing the PEG antifouling layer with bioactive host molecules. Nanostructures will then be implemented on the substrate in order to enhance the SFG signal through localized surface plasmon resonances (Lis et al., Adv Opt Mater 1:244–255, 2013). The ultimate goal will be to detect the SFG signature of the antigen/antibody recognition process at the interface of the above biosensing layer. The general chemical formula of the molecule investigated is C3H5S2 -(CH2)4 –C= O-NH-(CH2 -CH2 -O)n -CH3. The molecule studied here holds 7 polyethylene glycol units (n= 7). Former studies carried out by quartz crystal microbalance (QCM) and electrochemistry showed that molecules containing 7 PEG chains have the best antifouling properties. The SFG spectra present several vibrational modes (Sartenaer et al., Biosens Bioelectron 22:2179–2183, 2007; Cimatu et al., J Phys Chem C 112:14529–14537, 2008; Even et al., Macromolecules 39:9396–9401, 2006). The most significant contributions come from the CH2, O-CH2 and O-CH3 modes.

AB - Protein biochips are miniaturized biological sensors intended to analyze and characterize biomolecule interactions with high throughput. An important issue when developing such biochips is substrate passivation. The support is rendered inert in order to avoid non-specific adsorption. Strategic control of the non-specific protein adsorption can be achieved by creating a resistant self-assembled monolayer (SAM) based on polyethylene glycol (PEG). The degree of resistance depends on the PEG surface density, i.e. the number of PEG units the molecule contains. Infrared-visible sum-frequency generation (SFG) spectroscopy (Lambert et al., Appl Spectrosc Rev 40:103–145, 2005) is used to in-vestigate the vibrational fingerprint of a PEG self-assembled monolayer adsorbed on a flat platinum surface, in the 2,750–3,050 cm-1 frequency range. The objec-tive is to characterize the SFG baseline of the biosensor that will be further developed by mixing the PEG antifouling layer with bioactive host molecules. Nanostructures will then be implemented on the substrate in order to enhance the SFG signal through localized surface plasmon resonances (Lis et al., Adv Opt Mater 1:244–255, 2013). The ultimate goal will be to detect the SFG signature of the antigen/antibody recognition process at the interface of the above biosensing layer. The general chemical formula of the molecule investigated is C3H5S2 -(CH2)4 –C= O-NH-(CH2 -CH2 -O)n -CH3. The molecule studied here holds 7 polyethylene glycol units (n= 7). Former studies carried out by quartz crystal microbalance (QCM) and electrochemistry showed that molecules containing 7 PEG chains have the best antifouling properties. The SFG spectra present several vibrational modes (Sartenaer et al., Biosens Bioelectron 22:2179–2183, 2007; Cimatu et al., J Phys Chem C 112:14529–14537, 2008; Even et al., Macromolecules 39:9396–9401, 2006). The most significant contributions come from the CH2, O-CH2 and O-CH3 modes.

UR - http://www.scopus.com/inward/record.url?scp=84921483761&partnerID=8YFLogxK

U2 - 10.1007/978-94-017-9133-5_62

DO - 10.1007/978-94-017-9133-5_62

M3 - Article

VL - 68

SP - 545

EP - 546

JO - NATO Science for Peace and Security Series B: Physics and Biophysics

JF - NATO Science for Peace and Security Series B: Physics and Biophysics

SN - 1874-6500

ER -