Characterization of core/shell Cu/Ag nanopowders synthesized by electrochemistry and assessment of their impact on hemolysis, platelet aggregation, and coagulation on human blood for potential wound dressing use

Julie Laloy, Hélène Haguet, Lutfiye Alpan, Valérie Mancier, Jorge Mejia, Samuel Levi, Jean Michel Dogné, Stéphane Lucas, Céline Rousse, Patrick Fricoteaux

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Abstract

Copper/silver core/shell nanopowders with different metal ratio have been elaborated by electrochemistry (ultrasound-assisted electrolysis followed by a displacement reaction). Characterization was performed by several methods (X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, centrifugal liquid sedimentation, and zeta potential measurements). The mean diameter of all nanoparticles is around 10 nm. The impact of each nanopowder on hemolysis, platelet aggregation, and coagulation has been studied on whole human blood. Hemolysis assays were performed with spectrophotometric measurement and platelet aggregation, with light transmission aggregometry and was compared to Cu/Pt core/shell nanoparticles with similar size as negative control. Calibrated thrombin generation test has been used for a coagulation study. They neither impact platelet aggregation nor hemolysis and have a procoagulant effect whatever their composition (i.e., metal ratio). These results highlight that such nanopowders have a potential use in medical applications (e.g., wound dressing).

Original languageEnglish
Article number266
JournalJournal of Nanoparticle Research
Volume19
Issue number8
DOIs
Publication statusPublished - 1 Aug 2017

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Keywords

  • Biomedicine
  • Copper
  • Core-shell nanopowders
  • Procoagulant activity
  • Silver
  • Thrombin generation

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