Cdc18/CDC6 activates the Rad3-dependent checkpoint in the fission yeast

N. Fersht, D. Hermand, J. Hayles, P. Nurse

Research output: Contribution to journalArticle

Abstract

A screen for genes that can ectopically activate a Rad3-dependent checkpoint block over mitosis in fission yeast has identified the DNA replication initiation factor cdc18 (known as CDC6 in other organisms). Either a stabilized form of Cdc18, the Cdc18-T6A phosphorylation mutant, or overexpression of wild type Cdc18, activate the Rad3-dependent S-M checkpoint in the apparent absence of detectable replication structures and gross DNA damage. This cell cycle block relies on the Rad checkpoint pathway and requires Chk1 phosphorylation and activation. Unexpectedly, Cdc18-T6A induces changes in the mobility of Chromosome III, affecting the size of a restriction fragment containing rDNA repeats and producing aberrant nucleolar structures. Recombination events within the rDNA appear to contribute at least in part to the cell cycle delay. We propose that an elevated level of Cdc18 activates the Rad3-dependent checkpoint either directly or indirectly, and additionally causes expansion of the rDNA repeats on Chromosome III. © 2007 The Author(s).
Original languageEnglish
Pages (from-to)5323-5337
Number of pages15
JournalNucleic Acids Res
Volume35
Issue number16
DOIs
Publication statusPublished - 1 Aug 2007

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Schizosaccharomyces
Ribosomal DNA
Cell Cycle
Chromosomes
Phosphorylation
Peptide Initiation Factors
DNA Replication
Mitosis
Genetic Recombination
DNA Damage
Genes

Cite this

Fersht, N. ; Hermand, D. ; Hayles, J. ; Nurse, P. / Cdc18/CDC6 activates the Rad3-dependent checkpoint in the fission yeast. In: Nucleic Acids Res. 2007 ; Vol. 35, No. 16. pp. 5323-5337.
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Cdc18/CDC6 activates the Rad3-dependent checkpoint in the fission yeast. / Fersht, N.; Hermand, D.; Hayles, J.; Nurse, P.

In: Nucleic Acids Res, Vol. 35, No. 16, 01.08.2007, p. 5323-5337.

Research output: Contribution to journalArticle

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