TY - JOUR
T1 - Cationic lipids delay the transfer of plasmid DNA to lysosomes
AU - Wattiaux, R.
AU - Jadot, M.
AU - Laurent, N.
AU - Dubois, F.
AU - Wattiaux-De Coninck, Simone
N1 - Medline is the source for the MeSH terms of this document.
PY - 1996/10/14
Y1 - 1996/10/14
N2 - Plasmid S DNA, naked or associated with different cationic lipid preparations was injected to rats. Subcellular distribution of radioactivity in the liver one hour after injection, was established by centrifugation methods. Results show that at that time, S DNA has reached lysosomes. On the contrary, when S DNA was complexed with lipids, radioactivity remains located in organelles whose distribution after differential and isopycnic centrifugation, is clearly distinct from that of arylsulfatase, a lysosome marker enzyme. Injection of Triton WR 1339, a specific density perturbant of lysosomes, four days before S DNA injection causes a density decrease of radioactivity bearing structures, apparent one hour after naked S DNA injection but visible only after more than five hours, when S DNA associated with a cationic lipid is injected. These observations show that cationic lipids delay the transfer to lysosomes, of plasmid DNA taken up by the liver.
AB - Plasmid S DNA, naked or associated with different cationic lipid preparations was injected to rats. Subcellular distribution of radioactivity in the liver one hour after injection, was established by centrifugation methods. Results show that at that time, S DNA has reached lysosomes. On the contrary, when S DNA was complexed with lipids, radioactivity remains located in organelles whose distribution after differential and isopycnic centrifugation, is clearly distinct from that of arylsulfatase, a lysosome marker enzyme. Injection of Triton WR 1339, a specific density perturbant of lysosomes, four days before S DNA injection causes a density decrease of radioactivity bearing structures, apparent one hour after naked S DNA injection but visible only after more than five hours, when S DNA associated with a cationic lipid is injected. These observations show that cationic lipids delay the transfer to lysosomes, of plasmid DNA taken up by the liver.
UR - http://www.scopus.com/inward/record.url?scp=0030583566&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.1527
DO - 10.1006/bbrc.1996.1527
M3 - Article
AN - SCOPUS:0030583566
SN - 0006-291X
VL - 227
SP - 448
EP - 454
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -