TY - JOUR
T1 - Cathepsin B and N-acetyl-β-d-glucosaminidase in human synovial cells in culture: Effects of interleukin-l
AU - Lecomte, Véronique
AU - Knott, Isabelle
AU - Burton, Maggi
AU - Remacle, José
AU - Raes, Martine
PY - 1994
Y1 - 1994
N2 - Human synovial cells were cultured in vitro and tested for the activities of two lysosomal enzymes, cathepsin B and Nacetyl-β-d-glucosaminidase (NAGA) under various conditions. Unstimulated synovial cells display intracellular and extracellular activities of both enzymes. However, cathepsin B was secreted in a latent pepsin-activatable form, whereas NAGA was secreted in an active form. Most of the cell strains analysed secreted rather limited amounts of the enzymes (less than 25% of total activity); some strains, however were highly secretory, the secreted activity reaching up to 50% of total activity. Cells were then stimulated with human recombinant interleukin-l α (rhIL-lα) orβ. Only the levels of secreted NAGA were clearly increased. Results are to be interpreted in view of the role played by synovial cells and by the lysosomal enzymes they release in inflammatory joint diseases and it would be worthwhile in the future to check for secreted NAGA in various body fluids, such as the synovial fluid of the inflamed joint.
AB - Human synovial cells were cultured in vitro and tested for the activities of two lysosomal enzymes, cathepsin B and Nacetyl-β-d-glucosaminidase (NAGA) under various conditions. Unstimulated synovial cells display intracellular and extracellular activities of both enzymes. However, cathepsin B was secreted in a latent pepsin-activatable form, whereas NAGA was secreted in an active form. Most of the cell strains analysed secreted rather limited amounts of the enzymes (less than 25% of total activity); some strains, however were highly secretory, the secreted activity reaching up to 50% of total activity. Cells were then stimulated with human recombinant interleukin-l α (rhIL-lα) orβ. Only the levels of secreted NAGA were clearly increased. Results are to be interpreted in view of the role played by synovial cells and by the lysosomal enzymes they release in inflammatory joint diseases and it would be worthwhile in the future to check for secreted NAGA in various body fluids, such as the synovial fluid of the inflamed joint.
KW - Cathepsin B
KW - Human synovial cells
KW - Interleukin-l
KW - N-Acetyl-β-d-glucosaminidase
UR - http://www.scopus.com/inward/record.url?scp=0028168907&partnerID=8YFLogxK
U2 - 10.1016/0009-8981(94)90285-2
DO - 10.1016/0009-8981(94)90285-2
M3 - Article
C2 - 7988031
AN - SCOPUS:0028168907
SN - 0009-8981
VL - 228
SP - 143
EP - 159
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 2
ER -