Cathepsin B and N-acetyl-β-d-glucosaminidase in human synovial cells in culture: Effects of interleukin-l

Véronique Lecomte, Isabelle Knott, Maggi Burton, José Remacle, Martine Raes

Research output: Contribution to journalArticle

Abstract

Human synovial cells were cultured in vitro and tested for the activities of two lysosomal enzymes, cathepsin B and Nacetyl-β-d-glucosaminidase (NAGA) under various conditions. Unstimulated synovial cells display intracellular and extracellular activities of both enzymes. However, cathepsin B was secreted in a latent pepsin-activatable form, whereas NAGA was secreted in an active form. Most of the cell strains analysed secreted rather limited amounts of the enzymes (less than 25% of total activity); some strains, however were highly secretory, the secreted activity reaching up to 50% of total activity. Cells were then stimulated with human recombinant interleukin-l α (rhIL-lα) orβ. Only the levels of secreted NAGA were clearly increased. Results are to be interpreted in view of the role played by synovial cells and by the lysosomal enzymes they release in inflammatory joint diseases and it would be worthwhile in the future to check for secreted NAGA in various body fluids, such as the synovial fluid of the inflamed joint.

Original languageEnglish
Pages (from-to)143-159
Number of pages17
JournalClinica Chimica Acta
Volume228
Issue number2
DOIs
Publication statusPublished - 1994

Fingerprint

Hexosaminidases
Cathepsin B
Interleukins
Cell Culture Techniques
Enzymes
Joint Diseases
Pepsin A
Synovial Fluid
Body fluids
Body Fluids
Cultured Cells
Joints
Fluids
cathepsin N

Keywords

  • Cathepsin B
  • Human synovial cells
  • Interleukin-l
  • N-Acetyl-β-d-glucosaminidase

Cite this

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abstract = "Human synovial cells were cultured in vitro and tested for the activities of two lysosomal enzymes, cathepsin B and Nacetyl-β-d-glucosaminidase (NAGA) under various conditions. Unstimulated synovial cells display intracellular and extracellular activities of both enzymes. However, cathepsin B was secreted in a latent pepsin-activatable form, whereas NAGA was secreted in an active form. Most of the cell strains analysed secreted rather limited amounts of the enzymes (less than 25{\%} of total activity); some strains, however were highly secretory, the secreted activity reaching up to 50{\%} of total activity. Cells were then stimulated with human recombinant interleukin-l α (rhIL-lα) orβ. Only the levels of secreted NAGA were clearly increased. Results are to be interpreted in view of the role played by synovial cells and by the lysosomal enzymes they release in inflammatory joint diseases and it would be worthwhile in the future to check for secreted NAGA in various body fluids, such as the synovial fluid of the inflamed joint.",
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Cathepsin B and N-acetyl-β-d-glucosaminidase in human synovial cells in culture: Effects of interleukin-l. / Lecomte, Véronique; Knott, Isabelle; Burton, Maggi; Remacle, José; Raes, Martine.

In: Clinica Chimica Acta, Vol. 228, No. 2, 1994, p. 143-159.

Research output: Contribution to journalArticle

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