TY - JOUR
T1 - Carbohydrate-Lectin Interaction on Graphene-Coated Surface Plasmon Resonance (SPR) Interfaces
AU - Penezic, Abra
AU - Deokar, Geetanjali
AU - Vignaud, Dominique
AU - Pichonat, Emmanuelle
AU - Happy, Henri
AU - Subramanian, Palaniappan
AU - Gasparović, Blaženka
AU - Boukherroub, Rabah
AU - Szunerits, Sabine
PY - 2014
Y1 - 2014
N2 - The paper describes the detection of carbohydrate-lectin interaction on graphene-on-metal surface plasmon resonance (SPR) interfaces. Graphene-coated gold-based SPR interfaces were formed through the transfer of large-area graphene grown by chemical vapor deposition (CVD) on polycrystalline Cu foils. The method allowed successful transfer of single- and double-layered graphene sheets onto the SPR interfaces in a reproducible manner. Functionalization of the graphene interface with mannose was achieved by simple immersion into a mannose aqueous solution (100 mM), resulting in noncovalent interactions between the aromatic ring structure of graphene and mannose. The utility of the carbohydrate-modified graphene-on-gold interface for the selective and sensitive detection of carbohydrate-lectin interactions was demonstrated using model lectins from Lens culinaris (LC) and Triticum vulgaris (TV). While LC lectin binds specifically to mannopyranoside units, TV lectin has an affinity for N-acetyl glucosamine and sialic acid residues.
AB - The paper describes the detection of carbohydrate-lectin interaction on graphene-on-metal surface plasmon resonance (SPR) interfaces. Graphene-coated gold-based SPR interfaces were formed through the transfer of large-area graphene grown by chemical vapor deposition (CVD) on polycrystalline Cu foils. The method allowed successful transfer of single- and double-layered graphene sheets onto the SPR interfaces in a reproducible manner. Functionalization of the graphene interface with mannose was achieved by simple immersion into a mannose aqueous solution (100 mM), resulting in noncovalent interactions between the aromatic ring structure of graphene and mannose. The utility of the carbohydrate-modified graphene-on-gold interface for the selective and sensitive detection of carbohydrate-lectin interactions was demonstrated using model lectins from Lens culinaris (LC) and Triticum vulgaris (TV). While LC lectin binds specifically to mannopyranoside units, TV lectin has an affinity for N-acetyl glucosamine and sialic acid residues.
KW - Biosensors
KW - Graphene
KW - Lectins
KW - Mannose
KW - Surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=84900872671&partnerID=8YFLogxK
U2 - 10.1007/s11468-014-9686-3
DO - 10.1007/s11468-014-9686-3
M3 - Article
AN - SCOPUS:84900872671
SN - 1557-1955
VL - 9
SP - 677
EP - 683
JO - Plasmonics
JF - Plasmonics
IS - 3
ER -