BtpB, a novel Brucella TIR-containing effector protein with immune modulatory functions

Suzana P Salcedo; María I Marchesini; Clara Degos; Matthieu Terwagne; Kristine Von Bargen; Hubert Lepidi; Claudia K Herrmann; Thais L Santos Lacerda; Paul R C Imbert; Philippe Pierre; Lena Alexopoulou; Jean-Jacques Letesson; Diego J Comerci; Jean-Pierre Gorvel

doi:10.3389/fcimb.2013.00028

BtpB, a novel Brucella TIR-containing effector protein with immune modulatory functions

Suzana P Salcedo, María I Marchesini, Clara Degos, Matthieu Terwagne, Kristine Von Bargen, Hubert Lepidi, Claudia K Herrmann, Thais L Santos Lacerda, Paul R C Imbert, Philippe Pierre, Lena Alexopoulou, Jean-Jacques Letesson, Diego J Comerci, Jean-Pierre Gorvel

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Abstract

Several bacterial pathogens have TIR domain-containing proteins that contribute to their pathogenesis. We identified a second TIR-containing protein in Brucella spp. that we have designated BtpB. We show it is a potent inhibitor of TLR signaling, probably via MyD88. BtpB is a novel Brucella effector that is translocated into host cells and interferes with activation of dendritic cells. In vivo mouse studies revealed that BtpB is contributing to virulence and control of local inflammatory responses with relevance in the establishment of chronic brucellosis. Together, our results show that BtpB is a novel Brucella effector that plays a major role in the modulation of host innate immune response during infection.

Original language	English
Pages (from-to)	28
Journal	Frontiers in cellular and infection microbiology
Volume	3
DOIs	https://doi.org/10.3389/fcimb.2013.00028
Publication status	Published - 2013

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10.3389/fcimb.2013.00028

fcimb-03-00028Final published version, 4.09 MB

Cross-talk between Brucella and eucaryotic cells : impact of bacterial outer membrane vesicles (OMVs) on the endocytic pathway and other cellular functions
LETESSON, J., Jadot, M., Arnould, T., Raes, M. & De Bolle, X.
1/10/08 → 30/09/13
Project: Research

Biological Security Laboratory Level 3 (BL3)
Xavier De Bolle (Manager)
Technological Platform: Biological Security Laboratory Level 3
Facility/equipment: Technological Platform
Light Microscopy
Henri-Francois Renard (Manager), Alison Forrester (Manager), Catherine Demazy (Other) & Benjamin Ledoux (Manager)
Technological Platform Morphology - Imaging
Facility/equipment: Equipment

Caractérisation fonctionnelle de l'ORF BMEI1674 de Brucella Melitensis et du domaine TIR qu'elle contient. Relation entre B. Melitensis et le système immunitaire inné
Author: Terwagne, M. & Copin, R., 2006
Supervisor: Letesson, J. (Supervisor)
Student thesis: Master types › Master in Biochemistry and molecular and cellular Biology

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Salcedo, S. P., Marchesini, M. I., Degos, C., Terwagne, M., Von Bargen, K., Lepidi, H., Herrmann, C. K., Santos Lacerda, T. L., Imbert, P. R. C., Pierre, P., Alexopoulou, L., Letesson, J.-J., Comerci, D. J., & Gorvel, J.-P. (2013). BtpB, a novel Brucella TIR-containing effector protein with immune modulatory functions. Frontiers in cellular and infection microbiology, 3, 28. https://doi.org/10.3389/fcimb.2013.00028

@article{2514d6d4353143a9ae8eeccc4b488cbc,

title = "BtpB, a novel Brucella TIR-containing effector protein with immune modulatory functions",

abstract = "Several bacterial pathogens have TIR domain-containing proteins that contribute to their pathogenesis. We identified a second TIR-containing protein in Brucella spp. that we have designated BtpB. We show it is a potent inhibitor of TLR signaling, probably via MyD88. BtpB is a novel Brucella effector that is translocated into host cells and interferes with activation of dendritic cells. In vivo mouse studies revealed that BtpB is contributing to virulence and control of local inflammatory responses with relevance in the establishment of chronic brucellosis. Together, our results show that BtpB is a novel Brucella effector that plays a major role in the modulation of host innate immune response during infection.",

author = "Salcedo, {Suzana P} and Marchesini, {Mar{\'i}a I} and Clara Degos and Matthieu Terwagne and {Von Bargen}, Kristine and Hubert Lepidi and Herrmann, {Claudia K} and {Santos Lacerda}, {Thais L} and Imbert, {Paul R C} and Philippe Pierre and Lena Alexopoulou and Jean-Jacques Letesson and Comerci, {Diego J} and Jean-Pierre Gorvel",

year = "2013",

doi = "10.3389/fcimb.2013.00028",

language = "English",

volume = "3",

pages = "28",

journal = "Frontiers in cellular and infection microbiology",

issn = "2235-2988",

publisher = "Frontiers Media S. A.",

}

Salcedo, SP, Marchesini, MI, Degos, C, Terwagne, M, Von Bargen, K, Lepidi, H, Herrmann, CK, Santos Lacerda, TL, Imbert, PRC, Pierre, P, Alexopoulou, L, Letesson, J-J, Comerci, DJ & Gorvel, J-P 2013, 'BtpB, a novel Brucella TIR-containing effector protein with immune modulatory functions', Frontiers in cellular and infection microbiology, vol. 3, pp. 28. https://doi.org/10.3389/fcimb.2013.00028

TY - JOUR

T1 - BtpB, a novel Brucella TIR-containing effector protein with immune modulatory functions

AU - Salcedo, Suzana P

AU - Marchesini, María I

AU - Degos, Clara

AU - Terwagne, Matthieu

AU - Von Bargen, Kristine

AU - Lepidi, Hubert

AU - Herrmann, Claudia K

AU - Santos Lacerda, Thais L

AU - Imbert, Paul R C

AU - Pierre, Philippe

AU - Alexopoulou, Lena

AU - Letesson, Jean-Jacques

AU - Comerci, Diego J

AU - Gorvel, Jean-Pierre

PY - 2013

Y1 - 2013

N2 - Several bacterial pathogens have TIR domain-containing proteins that contribute to their pathogenesis. We identified a second TIR-containing protein in Brucella spp. that we have designated BtpB. We show it is a potent inhibitor of TLR signaling, probably via MyD88. BtpB is a novel Brucella effector that is translocated into host cells and interferes with activation of dendritic cells. In vivo mouse studies revealed that BtpB is contributing to virulence and control of local inflammatory responses with relevance in the establishment of chronic brucellosis. Together, our results show that BtpB is a novel Brucella effector that plays a major role in the modulation of host innate immune response during infection.

AB - Several bacterial pathogens have TIR domain-containing proteins that contribute to their pathogenesis. We identified a second TIR-containing protein in Brucella spp. that we have designated BtpB. We show it is a potent inhibitor of TLR signaling, probably via MyD88. BtpB is a novel Brucella effector that is translocated into host cells and interferes with activation of dendritic cells. In vivo mouse studies revealed that BtpB is contributing to virulence and control of local inflammatory responses with relevance in the establishment of chronic brucellosis. Together, our results show that BtpB is a novel Brucella effector that plays a major role in the modulation of host innate immune response during infection.

U2 - 10.3389/fcimb.2013.00028

DO - 10.3389/fcimb.2013.00028

M3 - Article

C2 - 23847770

SN - 2235-2988

VL - 3

SP - 28

JO - Frontiers in cellular and infection microbiology

JF - Frontiers in cellular and infection microbiology

ER -

BtpB, a novel Brucella TIR-containing effector protein with immune modulatory functions

Abstract

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Projects

Cross-talk between Brucella and eucaryotic cells : impact of bacterial outer membrane vesicles (OMVs) on the endocytic pathway and other cellular functions

Equipment

Biological Security Laboratory Level 3 (BL3)

Light Microscopy

Student theses

Caractérisation fonctionnelle de l'ORF BMEI1674 de Brucella Melitensis et du domaine TIR qu'elle contient. Relation entre B. Melitensis et le système immunitaire inné

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