Projects per year
Abstract
Brucellae are facultative intracellular pathogens. The recent development of methods and genetically engineered strains allowed the description of cell-cycle progression of. Brucella abortus, including unipolar growth and the ordered initiation of chromosomal replication. B. abortus cell-cycle progression is coordinated with intracellular trafficking in the endosomal compartments. Bacteria are first blocked at the G1 stage, growth and chromosome replication being resumed shortly before reaching the intracellular proliferation compartment. The control mechanisms of cell cycle are similar to those reported for the bacterium. Caulobacter crescentus, and they are crucial for survival in the host cell. The development of single-cell analyses could also be applied to other bacterial pathogens to investigate their cell-cycle progression during infection. Brucella abortus is a facultative intracellular pathogen that grows unipolarly and initiates the replication of its two chromosomes in a specific order.Bacteria at the G1 stage of the cell cycle, that is, before the initiation of their chromosomal replication, are preferentially internalized in host cells.Cell-cycle progression is coordinated with trafficking in the host cell, the endosomal stage being divided into two parts: a first, long, nongrowing part, and a second part in which growth and chromosomal replication are resumed.A cell-cycle control network, conserved with the model bacterium. Caulobacter crescentus, is essential for. B. abortus virulence.
Original language | English |
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Journal | Trends in Microbiology |
DOIs | |
Publication status | Published - 2015 |
Keywords
- Brucella
- Cell cycle
- Growth
- Intracellular infection
- Replication
Fingerprint
Dive into the research topics of 'Brucella abortus Cell Cycle and Infection Are Coordinated'. Together they form a unique fingerprint.-
PDR (FRSM) BRUCELL-CYCLE: BRUCELL-CYCLE
De Bolle, X., Willemart, K. & ONG, T. A. P.
31/12/14 → …
Project: Research
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Mécanismes de contrôle du cycle cellulaire de Brucella abortus en cours d'infection
1/07/13 → 30/06/15
Project: Research
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PAI n°P7/28 - MICRODEV: PAI n°P7/28 - MICRODEV: Role of developmental processes in the virulence of human pathogens : from molecular mechanisms to novel therapeutic targets
LETESSON, J., Coenye, T., Van Dijck, P., Van Melderen, L., HOLS, P. & De Bolle, X.
1/10/12 → 30/09/17
Project: Research
Equipment
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Biological Security Laboratory Level 3 (BL3)
Xavier De Bolle (Manager)
Technological Platform: Biological Security Laboratory Level 3Facility/equipment: Technological Platform
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Flow Cytometry
Jean-Yves Matroule (Manager) & Kevin Willemart (Other)
Technological Platform Morphology - ImagingFacility/equipment: Equipment
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Light Microscopy
Henri-Francois Renard (Manager), Alison Forrester (Manager), Catherine Demazy (Other) & Benjamin Ledoux (Manager)
Technological Platform Morphology - ImagingFacility/equipment: Equipment
Student theses
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Connecting the cell cycle and the pathogenicity of Brucella abortus. When a specific bacterial cell cycle arrest in G1 phase takes part in the colonization of mammalian cells
Author: Deghelt, M., 16 Dec 2013Supervisor: De Bolle, X. (Supervisor), Letesson, J. (Supervisor), Muylkens, B. (Jury), Van Doninck, K. (President), THANBICHLER, M. (External person) (Jury) & ROOP II, R. M. (External person) (Jury)
Student thesis: Doc types › Doctor of Sciences
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CtrA-dependant cell cycle regulation of Brucella abortus in culture and during infection
Author: Francis, N., 1 Dec 2015Supervisor: De Bolle, X. (Supervisor), Renard, P. (President), Crosson, S. (External person) (Jury), Biondi, E. (External person) (Jury) & Collet, J. (External person) (Jury)
Student thesis: Doc types › Doctor of Sciences
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On the link between Brucella abortus cell cycle and adaptation to its intracellular lifestyle
Author: Mullier, C., 24 Jan 2014Supervisor: De Bolle, X. (Supervisor), Renard, P. (President), Jadot, M. (Jury), Matroule, J. (Jury), Salcedo, S. P. (External person) (Jury) & Collet, J. (External person) (Jury)
Student thesis: Doc types › Doctor of Sciences