Biotinylated indoles as probes for indole-binding proteins

Eduard Dolusic, Mariusz Kowalczyk, Volker Magnus, Göran Sandberg, Jennifer Normanly

Research output: Contribution to journalArticle

Abstract

Biotinylated indoles were prepared for application as bifunctional probes for the detection of indolebinding proteins which participate in the life processes of humans, animals, plants, and bacteria. The indole nucleus was functionalized, at ring positions 3, 5, or 6, by attachment of a 2-aminoethyl group, which was then coupled to the carboxyl moiety of biotin, via a spacer composed of 3 or 4 concatenated â-alanine residues. The constructs thus obtained were able to inhibit tryptophanase activity, similarly to indole in a concentration-dependent manner. They also bound strongly to lysozyme and weakly to bovine and human serum albumins, in accordance with the known affinities of these proteins for indole and 3-(2-aminoethyl)indole (tryptamine). The biotin end of the protein-bound bifunctional probes could then be detected by coupling to (strept)avidin conjugated to alkaline phosphatase or horseradish peroxidase, followed by incubation with substrates which are converted by these enzymes to intensely colored or chemiluminescent products.
Original languageEnglish
Pages (from-to)152-162
Number of pages11
JournalBioconjugate Chemistry
Volume12
Issue number2
DOIs
Publication statusPublished - 2001
EventXth Conference on organic chemistry and biochemistry of young scientists - Liblice Castle, Czech Republic
Duration: 15 Jun 1998 → …

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Indoles
Carrier Proteins
Biotin
Proteins
Tryptophanase
Enzymes
Avidin
Phosphatases
Horseradish Peroxidase
Muramidase
Bovine Serum Albumin
Serum Albumin
Alanine
Alkaline Phosphatase
Bacteria
Animals
Substrates
indole
tryptamine

Cite this

Dolusic, E., Kowalczyk, M., Magnus, V., Sandberg, G., & Normanly, J. (2001). Biotinylated indoles as probes for indole-binding proteins. Bioconjugate Chemistry, 12(2), 152-162. https://doi.org/10.1021/bc000035o
Dolusic, Eduard ; Kowalczyk, Mariusz ; Magnus, Volker ; Sandberg, Göran ; Normanly, Jennifer. / Biotinylated indoles as probes for indole-binding proteins. In: Bioconjugate Chemistry. 2001 ; Vol. 12, No. 2. pp. 152-162.
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Dolusic, E, Kowalczyk, M, Magnus, V, Sandberg, G & Normanly, J 2001, 'Biotinylated indoles as probes for indole-binding proteins', Bioconjugate Chemistry, vol. 12, no. 2, pp. 152-162. https://doi.org/10.1021/bc000035o

Biotinylated indoles as probes for indole-binding proteins. / Dolusic, Eduard; Kowalczyk, Mariusz; Magnus, Volker; Sandberg, Göran; Normanly, Jennifer.

In: Bioconjugate Chemistry, Vol. 12, No. 2, 2001, p. 152-162.

Research output: Contribution to journalArticle

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AU - Dolusic, Eduard

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AB - Biotinylated indoles were prepared for application as bifunctional probes for the detection of indolebinding proteins which participate in the life processes of humans, animals, plants, and bacteria. The indole nucleus was functionalized, at ring positions 3, 5, or 6, by attachment of a 2-aminoethyl group, which was then coupled to the carboxyl moiety of biotin, via a spacer composed of 3 or 4 concatenated â-alanine residues. The constructs thus obtained were able to inhibit tryptophanase activity, similarly to indole in a concentration-dependent manner. They also bound strongly to lysozyme and weakly to bovine and human serum albumins, in accordance with the known affinities of these proteins for indole and 3-(2-aminoethyl)indole (tryptamine). The biotin end of the protein-bound bifunctional probes could then be detected by coupling to (strept)avidin conjugated to alkaline phosphatase or horseradish peroxidase, followed by incubation with substrates which are converted by these enzymes to intensely colored or chemiluminescent products.

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