Bcl-2 and Caspase 3 mRNA levels in the testes of gudgeon, Gobio gobio, exposed to ethinylestradiol (EE2)

S. Nadzialek, L.-M. Pigneur, B. Wéron, P. Kestemont

Research output: Contribution to journalArticle

Abstract

Apoptosis inhibition has been reported in the male reproductive tract of teleost fish exposed to 17β-estrogen or estrogen-like compounds. In order to understand the molecular mechanisms of cell death inhibition, this study examined 2 genes involved in the apoptotic pathway, Bcl-2 and Caspase 3, an anti-apoptotic and a pro-apoptotic genes, respectively. Partial cDNA sequences of Bcl-2 and Caspase 3 were cloned from gudgeon (Gobio gobio), a common European cyprinid fish. To follow mRNA levels of Bcl-2 and Caspase 3 under xenoestrogen exposure, we first performed an in vitro experiment on fish testis exposed to the most potent xenoestrogen found in the environment, ethinylestradiol (EE2). We further studied mRNA expression of both genes in the testis of fish exposed to xenoestrogens in situ. In the in vitro experiment, fragments of gudgeon testis were exposed for 21 days to 10, 10, 10, 1 and 10μg/L of EE2, as well as to positive (10μg/L of E2) and ethanol control medium. Results showed a significant induction of Bcl-2 mRNA at 10μg/L (p
Original languageEnglish
Pages (from-to)304-310
Number of pages7
JournalAquatic Toxicology
Volume98
Issue number3
DOIs
Publication statusPublished - 1 Jul 2010

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