TY - JOUR
T1 - Bcl-2 and Caspase 3 mRNA levels in the testes of gudgeon, Gobio gobio, exposed to ethinylestradiol (EE2)
AU - Nadzialek, S.
AU - Pigneur, L.-M.
AU - Wéron, B.
AU - Kestemont, P.
N1 - MEDLINE® is the source for the MeSH terms of this document.
PY - 2010/7/1
Y1 - 2010/7/1
N2 - Apoptosis inhibition has been reported in the male reproductive tract of teleost fish exposed to 17β-estrogen or estrogen-like compounds. In order to understand the molecular mechanisms of cell death inhibition, this study examined 2 genes involved in the apoptotic pathway, Bcl-2 and Caspase 3, an anti-apoptotic and a pro-apoptotic genes, respectively. Partial cDNA sequences of Bcl-2 and Caspase 3 were cloned from gudgeon (Gobio gobio), a common European cyprinid fish. To follow mRNA levels of Bcl-2 and Caspase 3 under xenoestrogen exposure, we first performed an in vitro experiment on fish testis exposed to the most potent xenoestrogen found in the environment, ethinylestradiol (EE2). We further studied mRNA expression of both genes in the testis of fish exposed to xenoestrogens in situ. In the in vitro experiment, fragments of gudgeon testis were exposed for 21 days to 10, 10, 10, 1 and 10μg/L of EE2, as well as to positive (10μg/L of E2) and ethanol control medium. Results showed a significant induction of Bcl-2 mRNA at 10μg/L (p
AB - Apoptosis inhibition has been reported in the male reproductive tract of teleost fish exposed to 17β-estrogen or estrogen-like compounds. In order to understand the molecular mechanisms of cell death inhibition, this study examined 2 genes involved in the apoptotic pathway, Bcl-2 and Caspase 3, an anti-apoptotic and a pro-apoptotic genes, respectively. Partial cDNA sequences of Bcl-2 and Caspase 3 were cloned from gudgeon (Gobio gobio), a common European cyprinid fish. To follow mRNA levels of Bcl-2 and Caspase 3 under xenoestrogen exposure, we first performed an in vitro experiment on fish testis exposed to the most potent xenoestrogen found in the environment, ethinylestradiol (EE2). We further studied mRNA expression of both genes in the testis of fish exposed to xenoestrogens in situ. In the in vitro experiment, fragments of gudgeon testis were exposed for 21 days to 10, 10, 10, 1 and 10μg/L of EE2, as well as to positive (10μg/L of E2) and ethanol control medium. Results showed a significant induction of Bcl-2 mRNA at 10μg/L (p
UR - http://www.scopus.com/inward/record.url?scp=77953686340&partnerID=8YFLogxK
U2 - 10.1016/j.aquatox.2010.02.016
DO - 10.1016/j.aquatox.2010.02.016
M3 - Article
AN - SCOPUS:77953686340
SN - 0166-445X
VL - 98
SP - 304
EP - 310
JO - Aquatic Toxicology
JF - Aquatic Toxicology
IS - 3
ER -