TY - JOUR
T1 - Artefactual effects of oxygen on cell culture models of cellular senescence and stem cell biology
AU - Toussaint, Olivier
AU - Weemaels, Geoffroy
AU - Debacq-Chainiaux, Florence
AU - Scharffetter-Kochanek, Karin
AU - Wlaschek, Meinhard
PY - 2011/2/1
Y1 - 2011/2/1
N2 - In life sciences, modelling of the in vivo conditions using in vitro models is an important tool to generate knowledge. Although aerobic organisms including mammals depend on accurate oxygen tension, mimicking physiological conditions in cell culture experiments is not very common. Due to the need for simple technical and experimental design, the requirement for simulating the in vivo oxygen tension parameters has been neglected over long time. Fortunately, due to increasing knowledge in recent years the attention has shifted towards this scientific demand. In this short review, we summarize data substantiating the necessity to adequately mimic physiological oxygen tension using cell culture models in life science research. © 2010 Wiley-Liss, Inc.
AB - In life sciences, modelling of the in vivo conditions using in vitro models is an important tool to generate knowledge. Although aerobic organisms including mammals depend on accurate oxygen tension, mimicking physiological conditions in cell culture experiments is not very common. Due to the need for simple technical and experimental design, the requirement for simulating the in vivo oxygen tension parameters has been neglected over long time. Fortunately, due to increasing knowledge in recent years the attention has shifted towards this scientific demand. In this short review, we summarize data substantiating the necessity to adequately mimic physiological oxygen tension using cell culture models in life science research. © 2010 Wiley-Liss, Inc.
UR - http://www.scopus.com/inward/record.url?scp=78649653362&partnerID=8YFLogxK
U2 - 10.1002/jcp.22416
DO - 10.1002/jcp.22416
M3 - Article
C2 - 20857403
SN - 1097-4652
VL - 226
SP - 315
EP - 321
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 2
ER -