Application of a clot-based assay to measure the procoagulant activity of stored allogeneic red blood cell concentrates

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Abstract

BACKGROUND: Thrombotic effects are possible complications of red blood cell transfusion. The generation and accumulation of procoagulant red blood cell extracellular vesicles during storage may play an important role in these thrombotic effects. The objective of this study was to assess the value of a simple phospholipid-dependent clot-based assay (STA(®)-Procoag-PPL) to estimate the procoagulant activity of stored red blood cells and changes in this activity during storage of the blood component.

MATERIALS AND METHODS: Extracellular vesicles from 12 red blood cell concentrates were isolated at 13 storage time-points and characterised by quantitative and functional methods: the degree of haemolysis (direct spectrophotometry), the quantification and determination of cellular origin (flow cytometry) and the procoagulant activity (thrombin generation and STA(®)-Procoag-PPL assays) were assessed.

RESULTS: The mean clotting time of extracellular vesicles isolated from red blood cell concentrates decreased from 117.2±3.6 sec on the day of collection to 33.8±1.3 sec at the end of the storage period. This illustrates the phospholipid-dependent procoagulant activity of these extracellular vesicles, as confirmed by thrombin generation. Results of the peak of thrombin and the STA(®)-Procoag-PPL were well correlated (partial r=-0.41. p<0.001). In parallel, an exponential increase of the number of red blood cell-derived extracellular vesicles from 1,779/μL to 218,451/μL was observed.

DISCUSSION: The STA(®)-Procoag-PPL is a potentially useful technique for assessing the procoagulant activity of a red blood cell concentrate.

LanguageEnglish
Pages1-10
Number of pages10
JournalBlood transfusion = Trasfusione del sangue
DOIs
StateE-pub ahead of print - 2017
Externally publishedYes

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Erythrocytes
Thrombin
Phospholipids
Erythrocyte Transfusion
Spectrophotometry
Hemolysis
Flow Cytometry
Extracellular Vesicles

Keywords

  • Journal Article

Cite this

@article{11560ce46829432590fa4059e1c711c3,
title = "Application of a clot-based assay to measure the procoagulant activity of stored allogeneic red blood cell concentrates",
abstract = "BACKGROUND: Thrombotic effects are possible complications of red blood cell transfusion. The generation and accumulation of procoagulant red blood cell extracellular vesicles during storage may play an important role in these thrombotic effects. The objective of this study was to assess the value of a simple phospholipid-dependent clot-based assay (STA({\circledR})-Procoag-PPL) to estimate the procoagulant activity of stored red blood cells and changes in this activity during storage of the blood component.MATERIALS AND METHODS: Extracellular vesicles from 12 red blood cell concentrates were isolated at 13 storage time-points and characterised by quantitative and functional methods: the degree of haemolysis (direct spectrophotometry), the quantification and determination of cellular origin (flow cytometry) and the procoagulant activity (thrombin generation and STA({\circledR})-Procoag-PPL assays) were assessed.RESULTS: The mean clotting time of extracellular vesicles isolated from red blood cell concentrates decreased from 117.2±3.6 sec on the day of collection to 33.8±1.3 sec at the end of the storage period. This illustrates the phospholipid-dependent procoagulant activity of these extracellular vesicles, as confirmed by thrombin generation. Results of the peak of thrombin and the STA({\circledR})-Procoag-PPL were well correlated (partial r=-0.41. p<0.001). In parallel, an exponential increase of the number of red blood cell-derived extracellular vesicles from 1,779/μL to 218,451/μL was observed.DISCUSSION: The STA({\circledR})-Procoag-PPL is a potentially useful technique for assessing the procoagulant activity of a red blood cell concentrate.",
keywords = "Journal Article",
author = "B{\'e}rang{\`e}re Devalet and Adeline Wannez and Nicolas Bailly and Lutfiye Alpan and Damien Gheldof and Jonathan Douxfils and V{\'e}ronique Deneys and Beno{\^i}t Bihin and Bernard Chatelain and Jean-Michel Dogn{\'e} and Christian Chatelain and Fran{\cc}ois Mullier",
year = "2017",
doi = "10.2450/2017.0230-16",
language = "English",
pages = "1--10",
journal = "Blood transfusion = Trasfusione del sangue",
issn = "1723-2007",
publisher = "SIMTI Servizi",

}

TY - JOUR

T1 - Application of a clot-based assay to measure the procoagulant activity of stored allogeneic red blood cell concentrates

AU - Devalet,Bérangère

AU - Wannez,Adeline

AU - Bailly,Nicolas

AU - Alpan,Lutfiye

AU - Gheldof,Damien

AU - Douxfils,Jonathan

AU - Deneys,Véronique

AU - Bihin,Benoît

AU - Chatelain,Bernard

AU - Dogné,Jean-Michel

AU - Chatelain,Christian

AU - Mullier,François

PY - 2017

Y1 - 2017

N2 - BACKGROUND: Thrombotic effects are possible complications of red blood cell transfusion. The generation and accumulation of procoagulant red blood cell extracellular vesicles during storage may play an important role in these thrombotic effects. The objective of this study was to assess the value of a simple phospholipid-dependent clot-based assay (STA(®)-Procoag-PPL) to estimate the procoagulant activity of stored red blood cells and changes in this activity during storage of the blood component.MATERIALS AND METHODS: Extracellular vesicles from 12 red blood cell concentrates were isolated at 13 storage time-points and characterised by quantitative and functional methods: the degree of haemolysis (direct spectrophotometry), the quantification and determination of cellular origin (flow cytometry) and the procoagulant activity (thrombin generation and STA(®)-Procoag-PPL assays) were assessed.RESULTS: The mean clotting time of extracellular vesicles isolated from red blood cell concentrates decreased from 117.2±3.6 sec on the day of collection to 33.8±1.3 sec at the end of the storage period. This illustrates the phospholipid-dependent procoagulant activity of these extracellular vesicles, as confirmed by thrombin generation. Results of the peak of thrombin and the STA(®)-Procoag-PPL were well correlated (partial r=-0.41. p<0.001). In parallel, an exponential increase of the number of red blood cell-derived extracellular vesicles from 1,779/μL to 218,451/μL was observed.DISCUSSION: The STA(®)-Procoag-PPL is a potentially useful technique for assessing the procoagulant activity of a red blood cell concentrate.

AB - BACKGROUND: Thrombotic effects are possible complications of red blood cell transfusion. The generation and accumulation of procoagulant red blood cell extracellular vesicles during storage may play an important role in these thrombotic effects. The objective of this study was to assess the value of a simple phospholipid-dependent clot-based assay (STA(®)-Procoag-PPL) to estimate the procoagulant activity of stored red blood cells and changes in this activity during storage of the blood component.MATERIALS AND METHODS: Extracellular vesicles from 12 red blood cell concentrates were isolated at 13 storage time-points and characterised by quantitative and functional methods: the degree of haemolysis (direct spectrophotometry), the quantification and determination of cellular origin (flow cytometry) and the procoagulant activity (thrombin generation and STA(®)-Procoag-PPL assays) were assessed.RESULTS: The mean clotting time of extracellular vesicles isolated from red blood cell concentrates decreased from 117.2±3.6 sec on the day of collection to 33.8±1.3 sec at the end of the storage period. This illustrates the phospholipid-dependent procoagulant activity of these extracellular vesicles, as confirmed by thrombin generation. Results of the peak of thrombin and the STA(®)-Procoag-PPL were well correlated (partial r=-0.41. p<0.001). In parallel, an exponential increase of the number of red blood cell-derived extracellular vesicles from 1,779/μL to 218,451/μL was observed.DISCUSSION: The STA(®)-Procoag-PPL is a potentially useful technique for assessing the procoagulant activity of a red blood cell concentrate.

KW - Journal Article

U2 - 10.2450/2017.0230-16

DO - 10.2450/2017.0230-16

M3 - Article

SP - 1

EP - 10

JO - Blood transfusion = Trasfusione del sangue

T2 - Blood transfusion = Trasfusione del sangue

JF - Blood transfusion = Trasfusione del sangue

SN - 1723-2007

ER -