TY - JOUR
T1 - Anti–inflammatory and antioxidant properties of the ethanol extract of Clerodendrum cyrtophyllum turcz in copper sulfate‐induced inflammation in zebrafish
AU - Nguyen, Thu Hang
AU - Le, Hong Diep
AU - Kim, Thanh Nguyen Thi
AU - The, Hai Pham
AU - Nguyen, Thi Mai
AU - Cornet, Valérie
AU - Lambert, Jérôme
AU - Kestemont, Patrick
N1 - Funding Information:
Acknowledgments: This study was supported by ARES (Académie de Recherche et d’Enseignement Supérieur) and DGD (Direction Générale de la Coopération au Développement) through the funding of the project “Exploring the Medical and (Eco)‐toxicological Potential of Natural Extracts in North Vietnam”.
Funding Information:
Funding: This research was funded by ARES (Académie de Recherche et d’Enseignement Supérieur) and the Ministry of Cooperation (DGD (Direction Générale de la Coopération au Développement)) in Belgium.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/3
Y1 - 2020/3
N2 - Oxidative stress and inflammation are commonly present in many chronic diseases. These responses are closely related to pathophysiological processes. The inflammatory process can induce oxidative stress and vice versa through the activation of multiple pathways. Therefore, agents with antioxidant and/or anti-inflammatory activities are very useful in the treatment of many pathologies.
Clerodendrum cyrthophyllum Turcz, a plant belonging to the Verbenaceae family, is used in Vietnamese traditional medicine for treating migraine, hypertension, inflammation of the throat, and rheumatic arthritis. Despite its usefulness, studies on its biological properties are still scarce. In this study, ethanol extract (EE) of leaves of
C. cyrtophyllum showed protective activity against CuSO
4 toxicity. The protective activity was proven to relate to antioxidant and anti-inflammatory properties. EE exhibited relatively high antioxidant activity (IC
50 of 16.45 µg/mL) as measured by DPPH assay. In an in vivo anti-antioxidant test, three days post fertilization (dpf) zebrafish larvae were treated with different concentrations of EE for 1 h and then exposed to 10 µM CuSO
4 for 20 min to induce oxidative stress. Fluorescent probes were used to detect and quantify oxidative stress by measuring the fluorescent intensity (FI) in larvae. FI significantly decreased in the presence of EE at 5 and 20µg/mL, demonstrating EE's profound antioxidant effects, reducing or preventing oxidative stress from CuSO
4. Moreover, the co-administration of EE also protected zebrafish larvae against oxidative damage from CuSO
4 through down-regulation of
hsp70 and
gadd45bb expression and upregulation of
sod. Due to copper accumulation in zebrafish tissues, the damage and oxidative stress were exacerbated overtime, resulting in the upregulation of genes related to inflammatory processes such as
cox-2,
pla2,
c3a,
mpo, and pro- and anti-inflammatory cytokines (
il-1ß,
il-8,
tnf-α, and
il-10, respectively). However, the association of CuSO
4 with EE significantly decreased the expression of
cox-2,
pla2,
c3a,
mpo,
il-8, and
il-1ß. Taken together, the results suggest that EE has potent antioxidant and anti-inflammatory activities and may be useful in the treatment of various inflammatory diseases.
AB - Oxidative stress and inflammation are commonly present in many chronic diseases. These responses are closely related to pathophysiological processes. The inflammatory process can induce oxidative stress and vice versa through the activation of multiple pathways. Therefore, agents with antioxidant and/or anti-inflammatory activities are very useful in the treatment of many pathologies.
Clerodendrum cyrthophyllum Turcz, a plant belonging to the Verbenaceae family, is used in Vietnamese traditional medicine for treating migraine, hypertension, inflammation of the throat, and rheumatic arthritis. Despite its usefulness, studies on its biological properties are still scarce. In this study, ethanol extract (EE) of leaves of
C. cyrtophyllum showed protective activity against CuSO
4 toxicity. The protective activity was proven to relate to antioxidant and anti-inflammatory properties. EE exhibited relatively high antioxidant activity (IC
50 of 16.45 µg/mL) as measured by DPPH assay. In an in vivo anti-antioxidant test, three days post fertilization (dpf) zebrafish larvae were treated with different concentrations of EE for 1 h and then exposed to 10 µM CuSO
4 for 20 min to induce oxidative stress. Fluorescent probes were used to detect and quantify oxidative stress by measuring the fluorescent intensity (FI) in larvae. FI significantly decreased in the presence of EE at 5 and 20µg/mL, demonstrating EE's profound antioxidant effects, reducing or preventing oxidative stress from CuSO
4. Moreover, the co-administration of EE also protected zebrafish larvae against oxidative damage from CuSO
4 through down-regulation of
hsp70 and
gadd45bb expression and upregulation of
sod. Due to copper accumulation in zebrafish tissues, the damage and oxidative stress were exacerbated overtime, resulting in the upregulation of genes related to inflammatory processes such as
cox-2,
pla2,
c3a,
mpo, and pro- and anti-inflammatory cytokines (
il-1ß,
il-8,
tnf-α, and
il-10, respectively). However, the association of CuSO
4 with EE significantly decreased the expression of
cox-2,
pla2,
c3a,
mpo,
il-8, and
il-1ß. Taken together, the results suggest that EE has potent antioxidant and anti-inflammatory activities and may be useful in the treatment of various inflammatory diseases.
KW - Antioxidant
KW - Anti‐inflammation
KW - Clerodendrum cyrtophyllum
KW - CuSO4
KW - Oxidative stress
KW - Zebrafish larvae
UR - http://www.scopus.com/inward/record.url?scp=85079836465&partnerID=8YFLogxK
U2 - 10.3390/antiox9030192
DO - 10.3390/antiox9030192
M3 - Article
C2 - 32106612
AN - SCOPUS:85079836465
SN - 2076-3921
VL - 9
JO - Antioxidants
JF - Antioxidants
IS - 3
M1 - 192
ER -