Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes

Bernard Masereel; Mustapha Dinguizli; Nicolas Moniotte; Caroline Bouzin; Olivier Feron; Bernard Gallez; Thierry Vander Borght; Carine Michiels; Stephane Lucas

doi:10.1007/s11051-010-9908-3

Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes

Bernard Masereel, Mustapha Dinguizli, Nicolas Moniotte, Caroline Bouzin, Olivier Feron, Bernard Gallez, Thierry Vander Borght, Carine Michiels, Stephane Lucas

Research output: Contribution to journal › Article › peer-review

Abstract

Nanoscale materials are used in the biomedical field for magnetic resonance imaging, protein detection and drug/gene delivery. Gold nanoparticles (AuNPs) are particularly investigated in cancer treatment and imaging. In this study, we described a simple and reliable liquid method to coat AuNPs (diameter: 21 nm) layer-by-layer with alternative cationic polyallylamine and anionic polystyrenesulfonate. The C-terminal amino acid of the antibody directed against anti-bovine serum albumin was activated by EDC/NHS, and then condensed with the amino functions of the external polyallylamine layer. An ELISA test confirmed that the antigen recognition of the bioconjugate antibody was conserved. This AuNP coating and the covalently coupling could be used as a generic process for binding other specific antibodies, particularly those overexpressed in cancer cells and angiogenesis. © 2010 Springer Science+Business Media B.V.

Original language	English
Pages (from-to)	1573-1580
Number of pages	8
Journal	Journal of Nanoparticle Research
Volume	13
Issue number	4
DOIs	https://doi.org/10.1007/s11051-010-9908-3
Publication status	Published - 1 Apr 2011

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10.1007/s11051-010-9908-3

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title = "Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes",

abstract = "Nanoscale materials are used in the biomedical field for magnetic resonance imaging, protein detection and drug/gene delivery. Gold nanoparticles (AuNPs) are particularly investigated in cancer treatment and imaging. In this study, we described a simple and reliable liquid method to coat AuNPs (diameter: 21 nm) layer-by-layer with alternative cationic polyallylamine and anionic polystyrenesulfonate. The C-terminal amino acid of the antibody directed against anti-bovine serum albumin was activated by EDC/NHS, and then condensed with the amino functions of the external polyallylamine layer. An ELISA test confirmed that the antigen recognition of the bioconjugate antibody was conserved. This AuNP coating and the covalently coupling could be used as a generic process for binding other specific antibodies, particularly those overexpressed in cancer cells and angiogenesis. {\textcopyright} 2010 Springer Science+Business Media B.V.",

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AU - Masereel, Bernard

AU - Dinguizli, Mustapha

AU - Moniotte, Nicolas

AU - Bouzin, Caroline

AU - Feron, Olivier

AU - Gallez, Bernard

AU - Vander Borght, Thierry

AU - Michiels, Carine

AU - Lucas, Stephane

N1 - Publication code : RES. ACAD.

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N2 - Nanoscale materials are used in the biomedical field for magnetic resonance imaging, protein detection and drug/gene delivery. Gold nanoparticles (AuNPs) are particularly investigated in cancer treatment and imaging. In this study, we described a simple and reliable liquid method to coat AuNPs (diameter: 21 nm) layer-by-layer with alternative cationic polyallylamine and anionic polystyrenesulfonate. The C-terminal amino acid of the antibody directed against anti-bovine serum albumin was activated by EDC/NHS, and then condensed with the amino functions of the external polyallylamine layer. An ELISA test confirmed that the antigen recognition of the bioconjugate antibody was conserved. This AuNP coating and the covalently coupling could be used as a generic process for binding other specific antibodies, particularly those overexpressed in cancer cells and angiogenesis. © 2010 Springer Science+Business Media B.V.

AB - Nanoscale materials are used in the biomedical field for magnetic resonance imaging, protein detection and drug/gene delivery. Gold nanoparticles (AuNPs) are particularly investigated in cancer treatment and imaging. In this study, we described a simple and reliable liquid method to coat AuNPs (diameter: 21 nm) layer-by-layer with alternative cationic polyallylamine and anionic polystyrenesulfonate. The C-terminal amino acid of the antibody directed against anti-bovine serum albumin was activated by EDC/NHS, and then condensed with the amino functions of the external polyallylamine layer. An ELISA test confirmed that the antigen recognition of the bioconjugate antibody was conserved. This AuNP coating and the covalently coupling could be used as a generic process for binding other specific antibodies, particularly those overexpressed in cancer cells and angiogenesis. © 2010 Springer Science+Business Media B.V.

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Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes

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Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes

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UN SDGs

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Synthesis, Irradiation and Analysis of Materials (SIAM)

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