Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Sha Li; Sandra Bouchy; Sebastien Penninckx; Riccardo Marega; Ornella Fichera; Bernard Gallez; Olivier Feron; Philippe Martinive; Anne-Catherine Heuskin; Carine Michiels; Stéphane Lucas

doi:10.2217/nnm-2018-0161

Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Sha Li, Sandra Bouchy, Sebastien Penninckx, Riccardo Marega, Ornella Fichera, Bernard Gallez, Olivier Feron, Philippe Martinive, Anne-Catherine Heuskin, Carine Michiels, Stéphane Lucas

Research output: Contribution to journal › Article › peer-review

Abstract

AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.

MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.

RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

Original language	English
Pages (from-to)	317-333
Number of pages	17
Journal	Nanomedicine
Volume	14
Issue number	3
Early online date	2019
DOIs	https://doi.org/10.2217/nnm-2018-0161
Publication status	Published - 1 Feb 2019

Keywords

EGFR
gold nanoparticles
nanoparticle uptake
protontherapy
radiosentizing effects

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10.2217/nnm-2018-0161

Link to publication in Scopus

Embargoed Document

2019_LiBouchyPenninckxMaregaFicheraHeuskinMichielsLucas_article
Final published version, 4.05 MB

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Morphology - Imaging
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - Imaging
Facility/equipment: Technological Platform
Synthesis, Irradiation and Analysis of Materials (SIAM)
Pierre Louette (Manager), Julien Colaux (Manager), Alexandre Felten (Manager) & Jorge Humberto Mejia Mendoza (Manager)
Technological Platform Synthesis, Irradiation and Analysis of Materials
Facility/equipment: Technological Platform
Transmission electron microscope
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - Imaging
Facility/equipment: Equipment

Cite this

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title = "Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy",

abstract = "AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.",

keywords = "EGFR, gold nanoparticles, nanoparticle uptake, protontherapy, radiosentizing effects",

author = "Sha Li and Sandra Bouchy and Sebastien Penninckx and Riccardo Marega and Ornella Fichera and Bernard Gallez and Olivier Feron and Philippe Martinive and Anne-Catherine Heuskin and Carine Michiels and St{\'e}phane Lucas",

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doi = "10.2217/nnm-2018-0161",

language = "English",

volume = "14",

pages = "317--333",

journal = "Nanomedicine",

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Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy. / Li, Sha; Bouchy, Sandra; Penninckx, Sebastien; Marega, Riccardo; Fichera, Ornella; Gallez, Bernard; Feron, Olivier; Martinive, Philippe; Heuskin, Anne-Catherine ; Michiels, Carine ; Lucas, Stéphane.

In: Nanomedicine, Vol. 14, No. 3, 01.02.2019, p. 317-333.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

AU - Li, Sha

AU - Bouchy, Sandra

AU - Penninckx, Sebastien

AU - Marega, Riccardo

AU - Fichera, Ornella

AU - Gallez, Bernard

AU - Feron, Olivier

AU - Martinive, Philippe

AU - Heuskin, Anne-Catherine

AU - Michiels, Carine

AU - Lucas, Stéphane

PY - 2019/2/1

Y1 - 2019/2/1

N2 - AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

AB - AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

KW - EGFR

KW - gold nanoparticles

KW - nanoparticle uptake

KW - protontherapy

KW - radiosentizing effects

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JO - Nanomedicine

JF - Nanomedicine

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Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Abstract

Keywords

Access to Document

Embargoed Document

Morphology - Imaging

Synthesis, Irradiation and Analysis of Materials (SIAM)

Transmission electron microscope

Cite this