Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Sha Li; Sandra Bouchy; Sebastien Penninckx; Riccardo Marega; Ornella Fichera; Bernard Gallez; Olivier Feron; Philippe Martinive; Anne-Catherine Heuskin; Carine Michiels; Stéphane Lucas

doi:10.2217/nnm-2018-0161

Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Sha Li, Sandra Bouchy, Sebastien Penninckx, Riccardo Marega, Ornella Fichera, Bernard Gallez, Olivier Feron, Philippe Martinive, Anne-Catherine Heuskin, Carine Michiels, Stéphane Lucas

Research output: Contribution to journal › Article › peer-review

Abstract

AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.

MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.

RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

Original language	English
Pages (from-to)	317-333
Number of pages	17
Journal	Nanomedicine
Volume	14
Issue number	3
Early online date	2019
DOIs	https://doi.org/10.2217/nnm-2018-0161
Publication status	Published - 1 Feb 2019

Keywords

EGFR
gold nanoparticles
nanoparticle uptake
protontherapy
radiosentizing effects
Microscopy, Electron, Transmission
Humans
Proton Therapy/methods
Metal Nanoparticles/chemistry
Cetuximab/chemistry
Gold/chemistry
Radiation-Sensitizing Agents/chemistry
Antibodies/chemistry
Cell Line, Tumor

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Morphology - Imaging
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - Imaging
Facility/equipment: Technological Platform
Synthesis, Irradiation and Analysis of Materials (SIAM)
Pierre Louette (Manager), Julien Colaux (Manager), Alexandre Felten (Manager) & Jorge Humberto Mejia Mendoza (Manager)
Technological Platform Synthesis, Irradiation and Analysis of Materials
Facility/equipment: Technological Platform
Transmission electron microscope
Charles Nicaise (Manager) & Francesca Cecchet (Manager)
Technological Platform Morphology - Imaging
Facility/equipment: Equipment

Cite this

@article{42494735c1d945eabfbfcd0dbe631adb,

title = "Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy",

abstract = "AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.",

keywords = "EGFR, gold nanoparticles, nanoparticle uptake, protontherapy, radiosentizing effects, Microscopy, Electron, Transmission, Humans, Proton Therapy/methods, Metal Nanoparticles/chemistry, Cetuximab/chemistry, Gold/chemistry, Radiation-Sensitizing Agents/chemistry, Antibodies/chemistry, Cell Line, Tumor",

author = "Sha Li and Sandra Bouchy and Sebastien Penninckx and Riccardo Marega and Ornella Fichera and Bernard Gallez and Olivier Feron and Philippe Martinive and Anne-Catherine Heuskin and Carine Michiels and St{\'e}phane Lucas",

note = "Funding Information: The authors thank the {\textquoteleft}Morphology-Imaging{\textquoteright} technological platform of the University of Namur for the electron microscopy. The authors also acknowledge the support of the SIAM platform. S{\'e}bastien Penninckx is a PhD fellow funded by the Belgian Funds for Scientific Research (FRS-FNRS, Belgium). Funding Information: TheraPlus project was supported by the Walloon Region (grant 1318075). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: {\textcopyright} 2019 2019 Future Medicine Ltd. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

year = "2019",

month = feb,

day = "1",

doi = "10.2217/nnm-2018-0161",

language = "English",

volume = "14",

pages = "317--333",

journal = "Nanomedicine",

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publisher = "Future Medicine Ltd.",

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Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy. / Li, Sha; Bouchy, Sandra; Penninckx, Sebastien; Marega, Riccardo; Fichera, Ornella; Gallez, Bernard; Feron, Olivier; Martinive, Philippe; Heuskin, Anne-Catherine ; Michiels, Carine ; Lucas, Stéphane.

In: Nanomedicine, Vol. 14, No. 3, 01.02.2019, p. 317-333.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

AU - Li, Sha

AU - Bouchy, Sandra

AU - Penninckx, Sebastien

AU - Marega, Riccardo

AU - Fichera, Ornella

AU - Gallez, Bernard

AU - Feron, Olivier

AU - Martinive, Philippe

AU - Heuskin, Anne-Catherine

AU - Michiels, Carine

AU - Lucas, Stéphane

N1 - Funding Information: The authors thank the ‘Morphology-Imaging’ technological platform of the University of Namur for the electron microscopy. The authors also acknowledge the support of the SIAM platform. Sébastien Penninckx is a PhD fellow funded by the Belgian Funds for Scientific Research (FRS-FNRS, Belgium). Funding Information: TheraPlus project was supported by the Walloon Region (grant 1318075). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: © 2019 2019 Future Medicine Ltd. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2019/2/1

Y1 - 2019/2/1

N2 - AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

AB - AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

KW - EGFR

KW - gold nanoparticles

KW - nanoparticle uptake

KW - protontherapy

KW - radiosentizing effects

KW - Microscopy, Electron, Transmission

KW - Humans

KW - Proton Therapy/methods

KW - Metal Nanoparticles/chemistry

KW - Cetuximab/chemistry

KW - Gold/chemistry

KW - Radiation-Sensitizing Agents/chemistry

KW - Antibodies/chemistry

KW - Cell Line, Tumor

UR - http://www.scopus.com/inward/record.url?scp=85061259208&partnerID=8YFLogxK

U2 - 10.2217/nnm-2018-0161

DO - 10.2217/nnm-2018-0161

M3 - Article

C2 - 30675822

VL - 14

SP - 317

EP - 333

JO - Nanomedicine

JF - Nanomedicine

SN - 1743-5889

IS - 3

ER -

Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Abstract

Keywords

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Fingerprint Dive into the research topics of 'Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy'. Together they form a unique fingerprint.

Morphology - Imaging

Synthesis, Irradiation and Analysis of Materials (SIAM)

Transmission electron microscope

Cite this