Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection

S. Loret, G. Nollevaux, R. Remacle, M. Klimek, I. Barakat, P. Deloyer, C. Grandfils, G. Dandrifosse

Research output: Contribution to journalArticle

Abstract

We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20±9.30% and 0.12±0.08% for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52±0.35 expressing a low diffusion of this poorly permeability marker.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume805
Issue number2
DOIs
Publication statusPublished - 15 Jun 2004

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Solid Phase Extraction
Gel permeation chromatography
Gel Chromatography
Permeability
Urine
Resins
polyethylene glycol 4000
polyethylene glycol 400
Healthy Volunteers

Keywords

  • Poly(ethylene glycol)

Cite this

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abstract = "We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20±9.30{\%} and 0.12±0.08{\%} for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52±0.35 expressing a low diffusion of this poorly permeability marker.",
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Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection. / Loret, S.; Nollevaux, G.; Remacle, R.; Klimek, M.; Barakat, I.; Deloyer, P.; Grandfils, C.; Dandrifosse, G.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 805, No. 2, 15.06.2004, p. 195-202.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection

AU - Loret, S.

AU - Nollevaux, G.

AU - Remacle, R.

AU - Klimek, M.

AU - Barakat, I.

AU - Deloyer, P.

AU - Grandfils, C.

AU - Dandrifosse, G.

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AB - We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20±9.30% and 0.12±0.08% for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52±0.35 expressing a low diffusion of this poorly permeability marker.

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