Advances in the Mechanistic Understanding of Iron Oxide Nanoparticles' Radiosensitizing Properties

Indiana Ternad; Sebastien Penninckx; Valentin Lecomte; Thomas Vangijzegem; Louise Conrard; Stéphane Lucas; Anne-Catherine Heuskin; Carine Michiels; Robert N Muller; Dimitri Stanicki; Sophie Laurent

doi:10.3390/nano13010201

Advances in the Mechanistic Understanding of Iron Oxide Nanoparticles' Radiosensitizing Properties

Indiana Ternad, Sebastien Penninckx, Valentin Lecomte, Thomas Vangijzegem, Louise Conrard, Stéphane Lucas, Anne-Catherine Heuskin, Carine Michiels, Robert N Muller, Dimitri Stanicki, Sophie Laurent

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Abstract

Among the plethora of nanosystems used in the field of theranostics, iron oxide nanoparticles (IONPs) occupy a central place because of their biocompatibility and magnetic properties. In this study, we highlight the radiosensitizing effect of two IONPs formulations (namely 7 nm carboxylated IONPs and PEG 5000-IONPs) on A549 lung carcinoma cells when exposed to 225 kV X-rays after 6 h, 24 h and 48 h incubation. The hypothesis that nanoparticles exhibit their radiosensitizing effect by weakening cells through the inhibition of detoxification enzymes was evidenced by thioredoxin reductase activity monitoring. In particular, a good correlation between the amplification effect at 2 Gy and the residual activity of thioredoxin reductase was observed, which is consistent with previous observations made for gold nanoparticles (NPs). This emphasizes that NP-induced radiosensitization does not result solely from physical phenomena but also results from biological events.

Original language	English
Article number	201
Journal	Nanomaterials
Volume	13
Issue number	1
DOIs	https://doi.org/10.3390/nano13010201
Publication status	Published - 2 Jan 2023

Keywords

biological mechanism
cancer therapy
iron oxide nanoparticles
radiosensitization
thioredoxin reductase
X-ray irradiation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/nano13010201

nanomaterials-13-00201Final published version, 2.08 MB

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title = "Advances in the Mechanistic Understanding of Iron Oxide Nanoparticles' Radiosensitizing Properties",

abstract = "Among the plethora of nanosystems used in the field of theranostics, iron oxide nanoparticles (IONPs) occupy a central place because of their biocompatibility and magnetic properties. In this study, we highlight the radiosensitizing effect of two IONPs formulations (namely 7 nm carboxylated IONPs and PEG 5000-IONPs) on A549 lung carcinoma cells when exposed to 225 kV X-rays after 6 h, 24 h and 48 h incubation. The hypothesis that nanoparticles exhibit their radiosensitizing effect by weakening cells through the inhibition of detoxification enzymes was evidenced by thioredoxin reductase activity monitoring. In particular, a good correlation between the amplification effect at 2 Gy and the residual activity of thioredoxin reductase was observed, which is consistent with previous observations made for gold nanoparticles (NPs). This emphasizes that NP-induced radiosensitization does not result solely from physical phenomena but also results from biological events. ",

keywords = "biological mechanism, cancer therapy, iron oxide nanoparticles, radiosensitization, thioredoxin reductase, X-ray irradiation",

author = "Indiana Ternad and Sebastien Penninckx and Valentin Lecomte and Thomas Vangijzegem and Louise Conrard and St{\'e}phane Lucas and Anne-Catherine Heuskin and Carine Michiels and Muller, {Robert N} and Dimitri Stanicki and Sophie Laurent",

note = "Funding Information: The authors acknowledge the UNamur technological platform SIAM and Tijani Tabarrant for their assistance with the irradiation facilities. This work has been supported by the financial contributions of the Fond National de la Recherche Scientifique (FNRS), the ARC Programs of the French Community of Belgium, COST actions, the Walloon region (ProtherWal and Interreg projects) and the ERA-NET EuroNanoMed (THERAGET) of the EU Horizon 2020. The authors would like to thank the Center for Microscopy and Molecular Imaging (CMMI, supported by European Regional Development Fund Wallonia). Publisher Copyright: {\textcopyright} 2023 by the authors.",

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month = jan,

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doi = "10.3390/nano13010201",

language = "English",

volume = "13",

journal = "Nanomaterials",

issn = "2079-4991",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

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T1 - Advances in the Mechanistic Understanding of Iron Oxide Nanoparticles' Radiosensitizing Properties

AU - Ternad, Indiana

AU - Penninckx, Sebastien

AU - Lecomte, Valentin

AU - Vangijzegem, Thomas

AU - Conrard, Louise

AU - Lucas, Stéphane

AU - Heuskin, Anne-Catherine

AU - Michiels, Carine

AU - Muller, Robert N

AU - Stanicki, Dimitri

AU - Laurent, Sophie

N1 - Funding Information: The authors acknowledge the UNamur technological platform SIAM and Tijani Tabarrant for their assistance with the irradiation facilities. This work has been supported by the financial contributions of the Fond National de la Recherche Scientifique (FNRS), the ARC Programs of the French Community of Belgium, COST actions, the Walloon region (ProtherWal and Interreg projects) and the ERA-NET EuroNanoMed (THERAGET) of the EU Horizon 2020. The authors would like to thank the Center for Microscopy and Molecular Imaging (CMMI, supported by European Regional Development Fund Wallonia). Publisher Copyright: © 2023 by the authors.

PY - 2023/1/2

Y1 - 2023/1/2

N2 - Among the plethora of nanosystems used in the field of theranostics, iron oxide nanoparticles (IONPs) occupy a central place because of their biocompatibility and magnetic properties. In this study, we highlight the radiosensitizing effect of two IONPs formulations (namely 7 nm carboxylated IONPs and PEG 5000-IONPs) on A549 lung carcinoma cells when exposed to 225 kV X-rays after 6 h, 24 h and 48 h incubation. The hypothesis that nanoparticles exhibit their radiosensitizing effect by weakening cells through the inhibition of detoxification enzymes was evidenced by thioredoxin reductase activity monitoring. In particular, a good correlation between the amplification effect at 2 Gy and the residual activity of thioredoxin reductase was observed, which is consistent with previous observations made for gold nanoparticles (NPs). This emphasizes that NP-induced radiosensitization does not result solely from physical phenomena but also results from biological events.

AB - Among the plethora of nanosystems used in the field of theranostics, iron oxide nanoparticles (IONPs) occupy a central place because of their biocompatibility and magnetic properties. In this study, we highlight the radiosensitizing effect of two IONPs formulations (namely 7 nm carboxylated IONPs and PEG 5000-IONPs) on A549 lung carcinoma cells when exposed to 225 kV X-rays after 6 h, 24 h and 48 h incubation. The hypothesis that nanoparticles exhibit their radiosensitizing effect by weakening cells through the inhibition of detoxification enzymes was evidenced by thioredoxin reductase activity monitoring. In particular, a good correlation between the amplification effect at 2 Gy and the residual activity of thioredoxin reductase was observed, which is consistent with previous observations made for gold nanoparticles (NPs). This emphasizes that NP-induced radiosensitization does not result solely from physical phenomena but also results from biological events.

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KW - cancer therapy

KW - iron oxide nanoparticles

KW - radiosensitization

KW - thioredoxin reductase

KW - X-ray irradiation

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U2 - 10.3390/nano13010201

DO - 10.3390/nano13010201

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SN - 2079-4991

VL - 13

JO - Nanomaterials

JF - Nanomaterials

IS - 1

M1 - 201

ER -

Advances in the Mechanistic Understanding of Iron Oxide Nanoparticles' Radiosensitizing Properties

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Metallic Nanoparticles: A Useful Prompt Gamma Emitter for Range Monitoring in Proton Therapy?

Gold nanoparticles as a potent radiosensitizer: A transdisciplinary approach from physics to patient

Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

PROTHER-WAL: Proton Therapy Research in Wallonia

Synthesis, Irradiation and Analysis of Materials (SIAM)

Cite this

Advances in the Mechanistic Understanding of Iron Oxide Nanoparticles' Radiosensitizing Properties

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Research output

Metallic Nanoparticles: A Useful Prompt Gamma Emitter for Range Monitoring in Proton Therapy?

Gold nanoparticles as a potent radiosensitizer: A transdisciplinary approach from physics to patient

Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Projects

PROTHER-WAL: Proton Therapy Research in Wallonia

Equipment

Synthesis, Irradiation and Analysis of Materials (SIAM)

Cite this