A restriction map of virulence plasmid pVYE439-80 from a serogroup 9 Yersinia enterocolitica strain

Y Laroche, M van Bouchaute, G Cornelis

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A restriction map of the virulence plasmid pVYE439-80, isolated from Yersinia enterocolitica 439-80 (serogroup 9) was constructed for EcoRI, BamHI, SstII, and SmaI. The mapping was done after cloning of about two-thirds of the plasmid in Escherichia coli. The restriction pattern was compared to those obtained with plasmids isolated from Y. enterocolitica strains of serogroups 1, 3, and 5b. The restriction sites are particularly conserved in a region of about 25 kb. This region contains fragments that are also conserved in serogroup 8 strains [J. Heeseman, C. Keller, R. Morawa, N. Schmidt, H. J. Siemens, and R. Lauf (1983) J. Infect. Dis. 147, 107-115] and that were shown, in strains from this serogroup, to encode calcium dependency [D. A. Portnoy, H. Wolf-Watz, I. Bolin, A. B. Beeder, and S. Falkow, (1984) Infect. Immun. 43, 108-114].
    Original languageEnglish
    Pages (from-to)67-70
    Number of pages4
    JournalPlasmid
    Volume12
    Issue number1
    Publication statusPublished - 1984

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