A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium

Frédéric Silvestre, Javier Linares-Casenave, Serge I Doroshov, Dietmar Kültz

Research output: Contribution to journalArticle

Abstract

Temperature and selenium are two environmental parameters that potentially affect reproduction and stock recruitment of sturgeon in the San Francisco Bay/Delta Estuary. To identify proteins whose expression is modified by these environmental stressors, we performed a proteomic analysis on larval green and white sturgeons exposed to 18 or 26 degrees C and micro-injected with Seleno-L-Methionine to reach 8microgg(-)(1) selenium body burden, with L-Methionine as a control. Selenium and high temperature induced mortalities and abnormal morphologies in both species, with a higher mortality in green sturgeon. Larval proteins were separated by two-dimensional gel electrophoresis and differential abundances were detected following spot quantitation and hierarchical cluster analysis. In green sturgeon, 34 of 551 protein spots detected on gels showed a variation in abundance whereas in white sturgeon only 9 of 580 protein spots were differentially expressed (P
Original languageEnglish
Pages (from-to)3176-88
Number of pages13
JournalThe Science of the total environment
Volume408
Issue number16
DOIs
Publication statusPublished - 15 Jul 2010

Keywords

  • Selenium
  • Hot Temperature
  • Animals
  • Larva
  • Fishes
  • Proteomics
  • Electrophoresis, Gel, Two-Dimensional
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Water Pollutants, Chemical
  • Species Specificity
  • Cluster Analysis

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