Abstract
Temperature and selenium are two environmental parameters that potentially affect reproduction and stock recruitment of sturgeon in the San Francisco Bay/Delta Estuary. To identify proteins whose expression is modified by these environmental stressors, we performed a proteomic analysis on larval green and white sturgeons exposed to 18 or 26 degrees C and micro-injected with Seleno-L-Methionine to reach 8microgg(-)(1) selenium body burden, with L-Methionine as a control. Selenium and high temperature induced mortalities and abnormal morphologies in both species, with a higher mortality in green sturgeon. Larval proteins were separated by two-dimensional gel electrophoresis and differential abundances were detected following spot quantitation and hierarchical cluster analysis. In green sturgeon, 34 of 551 protein spots detected on gels showed a variation in abundance whereas in white sturgeon only 9 of 580 protein spots were differentially expressed (P
| Original language | English |
|---|---|
| Pages (from-to) | 3176-88 |
| Number of pages | 13 |
| Journal | The Science of the total environment |
| Volume | 408 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - 15 Jul 2010 |
Keywords
- Selenium
- Hot Temperature
- Animals
- Larva
- Fishes
- Proteomics
- Electrophoresis, Gel, Two-Dimensional
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Water Pollutants, Chemical
- Species Specificity
- Cluster Analysis
