A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium

Frédéric Silvestre; Javier Linares-Casenave; Serge I Doroshov; Dietmar Kültz

doi:10.1016/j.scitotenv.2010.04.005

A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium

Frédéric Silvestre, Javier Linares-Casenave, Serge I Doroshov, Dietmar Kültz

Namur Research Institute for Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Temperature and selenium are two environmental parameters that potentially affect reproduction and stock recruitment of sturgeon in the San Francisco Bay/Delta Estuary. To identify proteins whose expression is modified by these environmental stressors, we performed a proteomic analysis on larval green and white sturgeons exposed to 18 or 26 degrees C and micro-injected with Seleno-L-Methionine to reach 8microgg(-)(1) selenium body burden, with L-Methionine as a control. Selenium and high temperature induced mortalities and abnormal morphologies in both species, with a higher mortality in green sturgeon. Larval proteins were separated by two-dimensional gel electrophoresis and differential abundances were detected following spot quantitation and hierarchical cluster analysis. In green sturgeon, 34 of 551 protein spots detected on gels showed a variation in abundance whereas in white sturgeon only 9 of 580 protein spots were differentially expressed (P

Original language	English
Pages (from-to)	3176-88
Number of pages	13
Journal	The Science of the total environment
Volume	408
Issue number	16
DOIs	https://doi.org/10.1016/j.scitotenv.2010.04.005
Publication status	Published - 15 Jul 2010

Keywords

Selenium
Hot Temperature
Animals
Larva
Fishes
Proteomics
Electrophoresis, Gel, Two-Dimensional
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Water Pollutants, Chemical
Species Specificity
Cluster Analysis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.scitotenv.2010.04.005

Cite this

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title = "A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium",

abstract = "Temperature and selenium are two environmental parameters that potentially affect reproduction and stock recruitment of sturgeon in the San Francisco Bay/Delta Estuary. To identify proteins whose expression is modified by these environmental stressors, we performed a proteomic analysis on larval green and white sturgeons exposed to 18 or 26 degrees C and micro-injected with Seleno-L-Methionine to reach 8microgg(-)(1) selenium body burden, with L-Methionine as a control. Selenium and high temperature induced mortalities and abnormal morphologies in both species, with a higher mortality in green sturgeon. Larval proteins were separated by two-dimensional gel electrophoresis and differential abundances were detected following spot quantitation and hierarchical cluster analysis. In green sturgeon, 34 of 551 protein spots detected on gels showed a variation in abundance whereas in white sturgeon only 9 of 580 protein spots were differentially expressed (P",

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author = "Fr{\'e}d{\'e}ric Silvestre and Javier Linares-Casenave and Doroshov, {Serge I} and Dietmar K{\"u}ltz",

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T1 - A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium

AU - Silvestre, Frédéric

AU - Linares-Casenave, Javier

AU - Doroshov, Serge I

AU - Kültz, Dietmar

PY - 2010/7/15

Y1 - 2010/7/15

N2 - Temperature and selenium are two environmental parameters that potentially affect reproduction and stock recruitment of sturgeon in the San Francisco Bay/Delta Estuary. To identify proteins whose expression is modified by these environmental stressors, we performed a proteomic analysis on larval green and white sturgeons exposed to 18 or 26 degrees C and micro-injected with Seleno-L-Methionine to reach 8microgg(-)(1) selenium body burden, with L-Methionine as a control. Selenium and high temperature induced mortalities and abnormal morphologies in both species, with a higher mortality in green sturgeon. Larval proteins were separated by two-dimensional gel electrophoresis and differential abundances were detected following spot quantitation and hierarchical cluster analysis. In green sturgeon, 34 of 551 protein spots detected on gels showed a variation in abundance whereas in white sturgeon only 9 of 580 protein spots were differentially expressed (P

AB - Temperature and selenium are two environmental parameters that potentially affect reproduction and stock recruitment of sturgeon in the San Francisco Bay/Delta Estuary. To identify proteins whose expression is modified by these environmental stressors, we performed a proteomic analysis on larval green and white sturgeons exposed to 18 or 26 degrees C and micro-injected with Seleno-L-Methionine to reach 8microgg(-)(1) selenium body burden, with L-Methionine as a control. Selenium and high temperature induced mortalities and abnormal morphologies in both species, with a higher mortality in green sturgeon. Larval proteins were separated by two-dimensional gel electrophoresis and differential abundances were detected following spot quantitation and hierarchical cluster analysis. In green sturgeon, 34 of 551 protein spots detected on gels showed a variation in abundance whereas in white sturgeon only 9 of 580 protein spots were differentially expressed (P

KW - Selenium

KW - Hot Temperature

KW - Animals

KW - Larva

KW - Fishes

KW - Proteomics

KW - Electrophoresis, Gel, Two-Dimensional

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Water Pollutants, Chemical

KW - Species Specificity

KW - Cluster Analysis

U2 - 10.1016/j.scitotenv.2010.04.005

DO - 10.1016/j.scitotenv.2010.04.005

M3 - Article

C2 - 20435339

SN - 1879-1026

VL - 408

SP - 3176

EP - 3188

JO - The Science of the total environment

JF - The Science of the total environment

IS - 16

ER -

A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium

Abstract

Keywords

UN SDGs

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