A primordial RNA modification enzyme: The case of tRNA (m1A) methyltransferase

Martine Roovers, Johan Wouters, Janusz M. Bujnicki, Catherine Tricot, Victor Stalon, Henri Grosjean, Louis Droogmans

Research output: Contribution to journalArticle

Abstract

The modified nucleoside 1-methyladenosine (m1A) is found in the T-loop of many tRNAs from organisms belonging to the three domains of life (Eukaryota, Bacteria, Archaea). In the T-loop of eukaryotic and bacterial tRNAs, m1A is present at position 58, whereas in archaeal tRNAs it is present at position(s) 58 and/or 57, m1A57 being the obligatory intermediate in the biosynthesis of 1-methylinosine (m1I57). In yeast, the formation of m1A58 is catalysed by the essential tRNA (m1A58) methyltransferase (MTase), a tetrameric enzyme that is composed of two types of subunits (Gcd14p and Gcd10p), whereas in the bacterium Thermus thermophilus the enzyme is a homotetramer of the Trml polypeptide. Here, we report that the Trml enzyme from the archaeon Pyrococcus abyssi is also a homotetramer. However, unlike the bacterial site-specific Trml MTase, the P.abyssi enzyme is region-specific and catalyses the formation of m1A at two adjacent positions (57 and 58) in the T-loop of certain tRNAs. The stabilisation of P.abyssi Trml at extreme temperatures involves intersubunit disulphide bridges that reinforce the tetrameric oligomerisation, as revealed by biochemical and crystallographic evidences. The origin and evolution of m1A MTases is discussed in the context of different hypotheses of the tree of life.

Original languageEnglish
Pages (from-to)465-476
Number of pages12
JournalNucleic Acids Research
Volume32
Issue number2
DOIs
Publication statusPublished - 10 Mar 2004

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    Roovers, M., Wouters, J., Bujnicki, J. M., Tricot, C., Stalon, V., Grosjean, H., & Droogmans, L. (2004). A primordial RNA modification enzyme: The case of tRNA (m1A) methyltransferase. Nucleic Acids Research, 32(2), 465-476. https://doi.org/10.1093/nar/gkh191