A new method for the physical and genetic mapping of large plasmids: application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium

T Michiels, M Y Popoff, S Durviaux, C Coynault, G Cornelis

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A new method based on transposon-promoted deletions was used to generate a set of deletions in the 90 kb virulence plasmid of Salmonella typhimurium. The analysis of 16 deletion mutants allowed: (1) construction of the restriction map of the plasmid for HindIII, BamHI and BglII; (2) localisation of the plasmid region involved in virulence; (3) identification of two functional replicons on the plasmid.
    Original languageEnglish
    Pages (from-to)109-16
    Number of pages8
    JournalMicrobial pathogenesis
    Volume3
    Issue number2
    Publication statusPublished - 1987

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