Abstract
A new method based on transposon-promoted deletions was used to generate a set of deletions in the 90 kb virulence plasmid of Salmonella typhimurium. The analysis of 16 deletion mutants allowed: (1) construction of the restriction map of the plasmid for HindIII, BamHI and BglII; (2) localisation of the plasmid region involved in virulence; (3) identification of two functional replicons on the plasmid.
Original language | English |
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Pages (from-to) | 109-16 |
Number of pages | 8 |
Journal | Microbial pathogenesis |
Volume | 3 |
Issue number | 2 |
Publication status | Published - 1987 |