A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli

Nan Jiang, Guo Liang Ying, Ali K. Yetisen, Yunuen Montelongo, Ling Shen, Yu Xuan Xiao, Henk J. Busscher, Xiao Yu Yang, Bao Lian Su

Research output: Contribution to journalArticle

Abstract

Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by their exposure to a suspension of gold nanoparticles and l-cysteine to form a protecting functional layer to which porous silica layers were bound yielding pores with a diameter of 3.9 nm. The living cells within the bilayered nanoshells maintained high viability (96 ± 2%) as demonstrated by agar plating, even after five cycles of simultaneous exposure to high temperature (40 °C), lyticase and UV light. Moreover, yeast cells encapsulated in bilayered nanoshells were more recyclable than native cells due to nutrient storage in the shell.

LanguageEnglish
Pages4730-4735
Number of pages6
JournalChemical Science
Volume9
Issue number21
DOIs
Publication statusPublished - 1 Jan 2018

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Nanoshells
Yeast
Cells
Plating
Ultraviolet radiation
Silicon Dioxide
Gold
Nutrients
Agar
Cysteine
Suspensions
Nanoparticles
Temperature
lyticase

Cite this

Jiang, Nan ; Ying, Guo Liang ; Yetisen, Ali K. ; Montelongo, Yunuen ; Shen, Ling ; Xiao, Yu Xuan ; Busscher, Henk J. ; Yang, Xiao Yu ; Su, Bao Lian. / A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli. In: Chemical Science. 2018 ; Vol. 9, No. 21. pp. 4730-4735.
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abstract = "Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by their exposure to a suspension of gold nanoparticles and l-cysteine to form a protecting functional layer to which porous silica layers were bound yielding pores with a diameter of 3.9 nm. The living cells within the bilayered nanoshells maintained high viability (96 ± 2{\%}) as demonstrated by agar plating, even after five cycles of simultaneous exposure to high temperature (40 °C), lyticase and UV light. Moreover, yeast cells encapsulated in bilayered nanoshells were more recyclable than native cells due to nutrient storage in the shell.",
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Jiang, N, Ying, GL, Yetisen, AK, Montelongo, Y, Shen, L, Xiao, YX, Busscher, HJ, Yang, XY & Su, BL 2018, 'A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli' Chemical Science, vol. 9, no. 21, pp. 4730-4735. https://doi.org/10.1039/c8sc01130c

A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli. / Jiang, Nan; Ying, Guo Liang; Yetisen, Ali K.; Montelongo, Yunuen; Shen, Ling; Xiao, Yu Xuan; Busscher, Henk J.; Yang, Xiao Yu; Su, Bao Lian.

In: Chemical Science, Vol. 9, No. 21, 01.01.2018, p. 4730-4735.

Research output: Contribution to journalArticle

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