Transcriptional regulation in hypoxia : role of HIF-1

Several aspects of hypoxia-mediated regulation of gene transcription are investigated. We are investigating kinase pathways involved in HIF-1 phosphorylation. A new project is aimed to identify new proteins interacting with HIF-1 by a two-hybrid screen as well as by 2-D gels. We are also identifiying the amino acids involved in direct contacts with DNA through computational modeling and site-directed mutagenesis.