Télévie: Study of the roles of extracellular vesicles as miRNA transporters in diffuse large B-cell lymphoma

Extracellular vesicles (EVs) are potentially released by all cell types. Their roles in carcinogenic process are currently debated in the scientific community. Some studies describe EVs as carriers transporting cellular materials. By interacting with target cells, these vesicles would modify target cells’ phenotype and would be implicated in cancer processes such as angiogenesis, metastasis, transfer of chemotherapeutics resistances, hypercoagulable state and inflammation. Because of their location in biological fluids, a lot of studies tried to use these EVs as biomarker. This property might be of particular interest in diffuse large B cell lymphoma (DLBCL). Indeed, because of the heterogeneity in terms of phenotype and treatment response in this pathology, it is necessary to define new biomarkers enabling a better definition of DLBCL subtypes, a prognostic evaluation, a chemoresistance overview and an early detection of this cancer. The study of EVs could also enable a better understanding of DLBCL physiopathology and could lead to new treatments discovery. The objectives of this project are (i) to study the roles of EVs in chemotherapeutics resistance transfer, in procoagulation process and in
angiogenesis in DLBCL, (ii) to characterize nucleic acids (miRNA) in EVs linked with these cancer processes, (iii) to study circulating miRNA as biomarkers in plasma of DLBCL patients.